Hepatitis　and　arthritis　are　prevalent　inflammatory　diseases,　and　the　utilization　of　fluorogenic　probes　incorporating　hydrogen　sulfide　(H2S)　as　a　crucial　mediator　of　inflammation　presents　significant　opportunities　for　early　detection.　However,　the　poor　in　vivo　biodistribution　and　limited　targeted　efficacy　of　molecule　probes　for　inflammation　imaging　severely　impede　their　ability　to　differentiate　the　extent　of　inflammation　and　provide　real-time　monitoring　of　inflammatory　levels.　Therefore,　we　developed　a　highly　efficient　H2S-activated　near-infrared　(NIR)　fluorogenic　probe　(hCy-DNP)　for　real-time　tracking　and　capturing　fluctuations　in　H2S　levels　within　inflammatory　lesions.　hCy-DNP　demonstrates　an　exceptionally　sensitive　fluorescence　response　to　H2S　expression,　enabling　specific　differentiation　between　various　levels　of　lipopolysaccharide　(LPS)　-stimulated　early　hepatitis　models　in　situ,　while　also　facilitating　visual　monitoring　for　diagnosis　and　efficacy　evaluation　of　arthritis.　Therefore,　hCy-DNP　offers　an　innovative　tool　for　exploring　early　diagnosis　and　evaluating　treatment　effectiveness　across　diverse　inflammatory　diseases.　©　2024　The　Authors.　BMEMat　published　by　John　Wiley　&　Sons　Australia,　Ltd　on　behalf　of　Shandong　University.