In　the　conditions　of　room　temperature　and　low　DO,　the　wastewater　advanced　treatment　with　a　single　reactor　is　studied.　The　tests　show　the　synchronous　nitrification-anammox　reaction　for　municipal　wastewater　can　be　carried　out　through　inoculating　anammox　bacteria　to　the　reactor　with　nitrification　ability,　and　its　removal　rate　can　reach　92.4%.　The　advanced　treatment　of　domestic　wastewater　by　the　single　reactor　can　be　carried　out　after　inoculating　PAOs　again,　and　their　corresponding　removal　rate　can　be　93.6%,　77.1%,　99.3%,　respectively.　DO=1　mg/L　can　be　considered　as　the　indication　parameter　of　system　reaction　terminal.　There　is　good　linearity　correlativity　between　TN　and　NH4+-N　for　the　concentration　change　in　the　reaction　process.　The　correlation　coefficient　are　all　more　than　0.999.