The　computer　vision　with　the　laser　stripe　scanning　method　is　widely　used　in　the　macro　field　of　shape　reconstruction　and　measurement　for　the　objects　with　a　large-scale.　On　the　basis　of　the　macro　laser　stripe　scanning　method,　we　proposed　a　new　method　with　a　stereo　light　microscope　and　laser　stripe　scanning　to　be　used　in　the　micro　filed　of　shape　reconstruction　and　measurement　for　the　objects　with　a　micro-scale.　And　this　article　is　especially　concerned　with　the　problem　of　edge　detection　of　laser　stripes　located　on　microscope　images.　First,　we　analyzed　the　character　of　microscope　image　and　proposed　an　edge　detection　algorithm　for　these　laser　stripes　based　on　Canny　rule.　Then,　an　edge　filter　is　used　to　smooth　the　edge　burrs.　At　last,　the　Hermite　interpolation　method　is　used　to　link　the　broken　edges　and　output　continuous　and　smoothing　edge　contours　of　laser　stripes.　The　results　show　that　this　method　can　output　high-quality　edge　contours　and　is　suitable　in　the　edge　detection　of　laser　stripe　located　in　microscope　image.　©　2018　IEEE.