Inactivated　vaccines　are　widely　used　for　prevention　of　viral　disease.　Both　humoral　and　cellular　immune　responses　have　been　shown　to　play　important　roles　in　the　control　and　clearance　of　virus　infections.　CpG　motif　containing　oligodeoxynucleotides　(ODN)　have　recently　gained　considerable　interest　and　been　used　as　vaccine　adjuvant　due　to　their　potent　abilities　to　modulate　host　immune　response.　In　this　study,　CpG-ODN　adjuvant　and　inactivated　viral　particles　of　enterovirus　71　(EV71)　were　co-encapsulated　into　nanoparticles　(NP)　generated　by　using　protamine　sulfate　(PS)　and　carboxymethyl　beta-glucan　(CMG)　through　a　self-assembly　approach.　The　administration　of　EV71　nanovaccine　elicited　not　only　specific　anti-EV71　neutralizing　antibody　response,　but　also　cellular　immune　response　characterized　by　strong　productions　of　IFN-alpha　and　IFN-gamma.　The　results　suggest　that　CMG/PS-based　nanovehicles　hold　a　great　potential　to　be　a　novel　platform　for　nanovaccine　development　against　viral　disease.　(C)　2019　Elsevier　Inc.　All　rights　reserved.