Background:　Mass　spectrometry　is　a　tool　used　in　analytical　chemistry　to　identify　components　in　a　chemical　compound　and　it　is　of　tremendous　importance　in　the　field　of　biology　for　high　throughput　analysis　of　biomolecules,　among　which　protein　is　of　great　interest.　Objective:　Advancement　in　proteomics　based　on　mass　spectrometry　has　led　the　way　to　quantify　multiple　protein　complexes,　and　proteins　interactions　with　DNA/RNA　or　other　chemical　compounds　which　is　a　breakthrough　in　the　field　of　bioinformatics.　Methods:　Many　new　technologies　have　been　introduced　in　electrospray　ionization　(ESI)　and　Matrix-assisted　Laser　Desorption/Ionization　(MALDI)　techniques　which　have　enhanced　sensitivity,　resolution　and　many　other　key　features　for　the　characterization　of　proteins.　Results:　The　advent　of　ambient　mass　spectrometry　and　its　different　versions　like　Desorption　Electrospray　Ionization　(DESI),　DART　and　ELDI　has　brought　a　huge　revolution　in　proteomics　research.　Different　imaging　techniques　are　also　introduced　in　MS　to　map　proteins　and　other　significant　biomolecules.　These　drastic　developments　have　paved　the　way　to　analyze　large　proteins　of　＞200kDa　easily.　Conclusion:　Here,　we　discuss　the　recent　advancement　in　mass　spectrometry,　which　is　of　great　importance　and　it　could　lead　us　to　further　deep　analysis　of　the　molecules　from　different　perspectives　and　further　advancement　in　these　techniques　will　enable　us　to　find　better　ways　for　prediction　of　molecules　and　their　behavioral　properties.