This　paper　presents　a　new　hermetic　encapsulation　method　for　negative-pressure-driven　polydimethylsiloxane　(PDMS)　microfluidic　devices.　The　hermetic　materials　used　in　this　encapsulation　are　mainly　paraffin　wax　and　glass　which　are　not　active,　non-hazardous,　and　typically　used　as　sealing　materials　for　medicine　and　food.　Compared　with　negative-pressure-driven　devices　only　wrapped　in　air-tight　plastic　packages,　one　advantage　of　the　new　encapsulation　method　is　that　only　inlet　is　exposed　to　air　when　the　encapsulated　device　is　unpackaged,　as　a　result　of　that　microfluidic　devices　encapsulated　with　the　new　method　can　achieve　a　higher　driving　pressure,　a　slower　decline　in　pumping　flow　rate,　and　maintain　much　longer　working　times.　In　order　to　demonstrate　the　applications　of　these　encapsulated　devices,　micropumps　and　integrated　chips　were　designed,　fabricated,　and　tested　in　this　research.　The　back　pressure,　vertical　flow　rate,　the　efficiency,　the　channel　filling　time　and　the　reservoir　filling　time　were　tested　in　this　study.　The　proposed　encapsulation　technique　can　offer　advantages　in　expanding　the　applications　of　negative-pressure-driven　microfluidic　devices.