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The effect of electron acceptors on denitrifying phosphorus (P) removal and its community structures in A(2)O process treating domestic wastewater was investigated using DNA based stable-isotope probing (DNA-SIP). Stable denitrifying P removal in anoxic zone of A(2)O process was achieved with an average denitrifying P removal efficiency of 72%. Results of quantitative polymerase chain reaction indicated that clade IIF was the dominant clade for denitrifying P removal. NirS, NirK and NarG were the major functional genes for denitrification. Batch tests were conducted with unlabelled and C-13-labelled sodium acetate as carbon sources, which confirmed that oxygen, nitrate and nitrite can be used as electron acceptor. High throughput sequencing for C-13-DNA samples indicated that polyphosphate accumulating organisms (PAOs) were mainly affiliated with phylum Proteobacteria. Candidatus_Accumulibacter was the major genus using oxygen and nitrate as electron acceptors. Acinetobacter was the most dominant genus using nitrate and nitrite as electron acceptors. Unclassified_f_Rhodocyclaceae appeared in three P removal systems. In the EBPR process, not only anaerobic P-release and aerobic P-uptake, but also denitrifying P removal should be considered. Different types of PAOs corresponding to different electron acceptors interacted to ensure the stable performance of nitrogen and phosphorus removal.
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CHEMICAL ENGINEERING JOURNAL
ISSN: 1385-8947
Year: 2018
Volume: 334
Page: 2039-2049
1 5 . 1 0 0
JCR@2022
ESI Discipline: ENGINEERING;
ESI HC Threshold:156
JCR Journal Grade:1
Cited Count:
WoS CC Cited Count: 51
SCOPUS Cited Count: 53
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 7