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Author:

Guo, Yu (Guo, Yu.) | Zeng, Wei (Zeng, Wei.) (Scholars:曾薇) | Li, Ning (Li, Ning.) | Peng, Yongzhen (Peng, Yongzhen.) (Scholars:彭永臻)

Indexed by:

EI Scopus SCIE

Abstract:

The effect of electron acceptors on denitrifying phosphorus (P) removal and its community structures in A(2)O process treating domestic wastewater was investigated using DNA based stable-isotope probing (DNA-SIP). Stable denitrifying P removal in anoxic zone of A(2)O process was achieved with an average denitrifying P removal efficiency of 72%. Results of quantitative polymerase chain reaction indicated that clade IIF was the dominant clade for denitrifying P removal. NirS, NirK and NarG were the major functional genes for denitrification. Batch tests were conducted with unlabelled and C-13-labelled sodium acetate as carbon sources, which confirmed that oxygen, nitrate and nitrite can be used as electron acceptor. High throughput sequencing for C-13-DNA samples indicated that polyphosphate accumulating organisms (PAOs) were mainly affiliated with phylum Proteobacteria. Candidatus_Accumulibacter was the major genus using oxygen and nitrate as electron acceptors. Acinetobacter was the most dominant genus using nitrate and nitrite as electron acceptors. Unclassified_f_Rhodocyclaceae appeared in three P removal systems. In the EBPR process, not only anaerobic P-release and aerobic P-uptake, but also denitrifying P removal should be considered. Different types of PAOs corresponding to different electron acceptors interacted to ensure the stable performance of nitrogen and phosphorus removal.

Keyword:

DNA based stable-isotope probing (DNA-SIP) Electron acceptor High throughput sequencing Denitrifying phosphorus removal Community structures

Author Community:

  • [ 1 ] [Guo, Yu]Beijing Univ Technol, Dept Environm Engn, Natl Engn Lab Adv Municipal Wastewater Treatment, Beijing 100124, Peoples R China
  • [ 2 ] [Zeng, Wei]Beijing Univ Technol, Dept Environm Engn, Natl Engn Lab Adv Municipal Wastewater Treatment, Beijing 100124, Peoples R China
  • [ 3 ] [Li, Ning]Beijing Univ Technol, Dept Environm Engn, Natl Engn Lab Adv Municipal Wastewater Treatment, Beijing 100124, Peoples R China
  • [ 4 ] [Peng, Yongzhen]Beijing Univ Technol, Dept Environm Engn, Natl Engn Lab Adv Municipal Wastewater Treatment, Beijing 100124, Peoples R China

Reprint Author's Address:

  • 曾薇

    [Zeng, Wei]Beijing Univ Technol, Dept Environm Engn, Pingleyuan 100, Beijing 100124, Peoples R China

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Source :

CHEMICAL ENGINEERING JOURNAL

ISSN: 1385-8947

Year: 2018

Volume: 334

Page: 2039-2049

1 5 . 1 0 0

JCR@2022

ESI Discipline: ENGINEERING;

ESI HC Threshold:156

JCR Journal Grade:1

Cited Count:

WoS CC Cited Count: 51

SCOPUS Cited Count: 53

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 7

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