The　accurate　acquisition　of　road　traffic　states　is　the　most　important　basis　in　the　Intelligent　Transportation　Systems.　Though　the　technology　for　collectting　road　traffic　states　information　has　been　developed　greatly,　the　data　problems　such　as　data　invalidation　also　exist　and　have　not　been　effectively　settled.　So　the　acquisition　method　of　reference　sequences　of　road　traffic　running　characteristics　is　put　forward　in　this　paper.　Firstly,　the　road　traffic　states　information　template　is　designed.　Then　the　regularity　analysis　of　the　road　traffic　states　information　is　carried　out　based　on　the　Singular　Value　Decomposition　of　the　road　traffic　states　information　matrix　and　the　effective　classification　of　eigenflows　of　the　road　traffic　states　information.　Then　the　road　traffic　modes　are　divided　into　several　different　sub-modes　according　to　different　classification　identifications.　Finally　the　regularity　information　of　road　traffic　states　under　different　modes　is　abstracted　and　Reference　sequences　of　road　traffic　running　characteristics　are　effectively　designed　based　on　this　regularity　information.　Typical　expressways　in　Beijing　are　adopted　for　the　application　of　the　reference　sequences　of　road　traffic　running　characteristics　for　data　imputation.　The　results　prove　that　the　reference　sequences　of　road　traffic　running　characteristics　can　be　a　effective　complement　in　the　process　of　date　collection.　©　2016　IEEE.