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学者姓名：曾薇
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Abstract ：
一种生物正交氨基酸标记染色法联合荧光原位杂交检测活性污泥系统中活性聚磷菌的方法，属于污水生物处理技术领域。本发明使用L‑高炔丙基甘氨酸培养活性污泥中微生物，并通过生物正交氨基酸标记染色法对利用该氨基酸合成蛋白质的微生物进行荧光染色；采用荧光原位杂交技术使探针PAO846、PAO651、PAO462与荧光染色后的样品中聚磷菌目的基因进行杂交；利用流式细胞仪检测被荧光标记的细菌，通过圈门处理识别出活性聚磷菌区域，进而计算和分析活性聚磷菌占全菌的比例。本发明提供了一种经济、简便、可靠的污水处理系统活性污泥中活性聚磷菌的定量方法，为实际污水处理厂调控生物除磷条件提供了理论依据。
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| GB/T 7714 | 曾薇 , 孟庆安 , 刘宏军 et al. 一种生物正交氨基酸标记染色法联合荧光原位杂交技术检测活性污泥系统中活性聚磷菌的方法 : CN202310164818.6[P]. | 2023-02-26 . |
| MLA | 曾薇 et al. "一种生物正交氨基酸标记染色法联合荧光原位杂交技术检测活性污泥系统中活性聚磷菌的方法" : CN202310164818.6. | 2023-02-26 . |
| APA | 曾薇 , 孟庆安 , 刘宏军 , 彭永臻 . 一种生物正交氨基酸标记染色法联合荧光原位杂交技术检测活性污泥系统中活性聚磷菌的方法 : CN202310164818.6. | 2023-02-26 . |
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Abstract ：
This study presented a novel process for simultaneous biological nutrient removal and waste activated sludge reduction in one-stage municipal wastewater treating system without aeration on the basis of endogenous partial denitrification, anammox and Candidatus Phosphoribacter dominated biological phosphate removal with sludge reduction (EPDA/PSR). The effluent total inorganic nitrogen and phosphate concentrations were 0.1 mg/L and lower than detection limit (0.1 mg/L), respectively, without dosing external carbon sources. Compared with partial denitrification-anammox process, the daily sludge production in EPDA/PSR process reduced by 50.8 %. The percentage of intact cells measured by flow cytometry reduced from 92.4 % to 77.3 % with the increase of aromatic protein, DNA content of supernatant, soluble microbial byproduct-like substance and decline of particle size. The sludge stabilization structure was destructed, and intracellular organics were released, which resulted in the reduction of sludge production. Microbial community structure at transcriptional level indicated that really working Candidatus Phosphoribacter (phosphorus accumulating organisms with fermentation capacity), Candidatus Competibacter (endogenous partial denitrification bacteria) and Candidatus Brocadia (anammox bacteria) reached 68.4 %, 9.84 % and 8.02 %, respectively, and Candidatus Kuenenia (anammox bacteria) did not play a role in EPDA/PSR system. The novel one-stage EPDA/PSR process realized high efficient biological nutrient removal, achieved waste activated sludge reduction and saved 100 % of aeration energy consumption cost and external carbon source dosage cost, which effectively developed internal carbon source and provided a new idea for wastewater treatment.
Keyword ：
Biological phosphate removal Biological phosphate removal Nutrient removal Nutrient removal Anammox Anammox Biological wastewater treatment Biological wastewater treatment Endogenous partial denitrification Endogenous partial denitrification
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| GB/T 7714 | Fan, Zhiwei , Zeng, Wei , Jia, Yuan et al. A novel process for simultaneous biological nutrient removal and waste activated sludge reduction in one-stage system without aeration [J]. | CHEMICAL ENGINEERING JOURNAL , 2023 , 454 . |
| MLA | Fan, Zhiwei et al. "A novel process for simultaneous biological nutrient removal and waste activated sludge reduction in one-stage system without aeration" . | CHEMICAL ENGINEERING JOURNAL 454 (2023) . |
| APA | Fan, Zhiwei , Zeng, Wei , Jia, Yuan , Meng, Qingan , Liu, Hongjun , Liu, Hong et al. A novel process for simultaneous biological nutrient removal and waste activated sludge reduction in one-stage system without aeration . | CHEMICAL ENGINEERING JOURNAL , 2023 , 454 . |
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Abstract ：
The significance of inorganic carbon (IC) for anaerobic ammonium oxidation (anammox) bacteria has been verified. However, the regulation of metabolic pathways under IC stress is not clear, limiting the optimization of IC supply. In this study, the regulatory pathways at IC concentration of 5-150 mg/L were explored to achieve optimal control of IC. The results show that the changes of metabolic pathway under IC stress determined anammox characteristics. At IC concentration of 5 mg/L, the anammox activity distinctly decreased due to the guanosine tetraphosphate (ppGpp) -mediated regulation under IC limitation. With less than 15 mg/L of IC, the decrease of carbon fixation limited the biosynthesis of gluconeogenesis and amino acids, causing the decline of extracellular polymeric substance synthesis. With more than 50 mg/L of IC, the improvement of purine and pyrimidine metabolism enhanced the electron transport capacity and growth potential of anammox bacteria. This study provides metabolic insights into IC influence on anammox consortia and a novel method of IC concentration optimization using metabolomics analysis.
Keyword ：
Metabolomics Metabolomics Inorganic carbon Inorganic carbon Anaerobic ammonium oxidation Anaerobic ammonium oxidation Extracellular polymeric substance (EPS) Extracellular polymeric substance (EPS)
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| GB/T 7714 | Ma, Chenyang , Zeng, Wei , Li, Jianmin et al. Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 855 . |
| MLA | Ma, Chenyang et al. "Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process" . | SCIENCE OF THE TOTAL ENVIRONMENT 855 (2023) . |
| APA | Ma, Chenyang , Zeng, Wei , Li, Jianmin , Meng, Qingan , Peng, Yongzhen . Metabolomic pathway regulation to achieve optimal control of inorganic carbon in anammox process . | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 855 . |
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Abstract ：
Sulfate in wastewater can be reduced to sulfide and its impact on the stability of enhanced biological phosphorus removal (EBPR) is still unclear. In this study, the metabolic changes and subsequent recovery of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) were investigated at different sulfide concentrations. The results showed that the metabolic activity of PAOs and GAOs was mainly related to H2S concentration. Under anaerobic conditions, the catabolism of PAOs and GAOs was promoted at H2S concentrations below 79 mg/L S and 271 mg/L S, respectively, and inhibited above these concentrations; whereas anabolism was consistently inhibited in the presence of H2S. The phosphorus (P) release was also pH-dependent due to the intracellular free Mg2+ efflux from PAOs. H2S was more destructive to the esterase activity and membrane permeability of PAOs than those of GAOs and prompted intracellular free Mg2+ efflux of PAOs, resulting in worse aerobic metabolism and subsequent recovery of PAOs than GAOs. Additionally, sulfides facilitated the production of extracellular polymeric substances (EPS), especially tightly bound EPS. The amount of EPS in GAOs was significantly higher than that in PAOs. The above results indicated that sulfide had a stronger inhibition to PAOs than GAOs, and when sulfide was present, GAOs had a competitive advantage over PAOs in EBPR.
Keyword ：
Anaerobic metabolism Anaerobic metabolism Sulfide inhibition Sulfide inhibition IntracellularMg2+ IntracellularMg2+ Enhanced biological phosphorus removal Enhanced biological phosphorus removal Flow cytometry Flow cytometry
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| GB/T 7714 | Meng, Qingan , Zeng, Wei , Fan, Zhiwei et al. Sulfide inhibition on polyphosphate accumulating organisms and glycogen accumulating organisms: Cumulative inhibitory effect and recoverability [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 451 . |
| MLA | Meng, Qingan et al. "Sulfide inhibition on polyphosphate accumulating organisms and glycogen accumulating organisms: Cumulative inhibitory effect and recoverability" . | JOURNAL OF HAZARDOUS MATERIALS 451 (2023) . |
| APA | Meng, Qingan , Zeng, Wei , Fan, Zhiwei , Li, Shuangshuang , Peng, Yongzhen . Sulfide inhibition on polyphosphate accumulating organisms and glycogen accumulating organisms: Cumulative inhibitory effect and recoverability . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 451 . |
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Abstract ：
Natural abundance isotope fractionation properties have become the most effective way to explore nitrogen transformations of biological nitrogen removal from wastewater. The migration and transformation characteristics of N and O elements in the shortcut nitrification were analyzed using the N and O dual isotopic fractionation technique. The effects of dissolved oxygen (DO) and temperature changes on the performance of shortcut nitrification and isotopic fractionation were investigated. The fractionation characteristics of N and O elements during shortcut nitrification were explored by adjusting DO concentration (0.2-0.4, 1-1.2 and 3-4 mg/L) and temperature (33 +/- 1 degrees C, 25 +/- 1 degrees C and 18 +/- 1 degrees C). Both delta N-15(NO2) and delta O-18(NO2) showed a gradually increasing trend with the accumulation of NO2--N, and the fractionation effects induced by temperature were significantly higher than those by DO. The higher the temperature, the more significant the increase in delta N-15(NO2); the higher the DO, the more remarkable the increase in delta O-18(NO2), while delta N-15(NO2): delta O-18(NO2) was maintained at 0.77-6.45. The O-18-labeled H2O was successfully transferred to NO2--N, and the replacement of O element was as high as 100 %, indicating that DO and H2O simultaneously participated in the shortcut nitrification process. The dynamic changes in isotope fractionation effects can be successfully applied to reveal the performance and mechanism of shortcut nitrification.
Keyword ：
Domestic wastewater Domestic wastewater Nitrification performance Nitrification performance O-18-labeled H2O O-18-labeled H2O Shortcut nitrification Shortcut nitrification Dual isotopes fractionation Dual isotopes fractionation
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| GB/T 7714 | Liu, Hong , Zeng, Wei , Zhan, Mengjia et al. Revealing the ammonia oxidation process and shortcut nitrification performance using nitrogen and oxygen isotope fractionation effect [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 912 . |
| MLA | Liu, Hong et al. "Revealing the ammonia oxidation process and shortcut nitrification performance using nitrogen and oxygen isotope fractionation effect" . | SCIENCE OF THE TOTAL ENVIRONMENT 912 (2023) . |
| APA | Liu, Hong , Zeng, Wei , Zhan, Mengjia , Li, Jianmin . Revealing the ammonia oxidation process and shortcut nitrification performance using nitrogen and oxygen isotope fractionation effect . | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 912 . |
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Abstract ：
本发明公开了一种从生活污水中回收羟基磷灰石颗粒及自养脱氮的装置与方法。本发明将磷酸盐矿化磷回收工艺与半短程硝化厌氧氨氧化自养脱氮工艺相结合，通过向厌氧氨氧化耦合磷酸盐矿化反应器内投加CaCl2，形成以羟基磷灰石等磷酸盐结晶为内核的厌氧氨氧化颗粒污泥。本发明不仅能够回收宝贵的磷资源，同时有利于厌氧氨氧化菌的持留与富集，最终提高处理系统的脱氮性能和运行的稳定性。脱氮效率提升至90％以上，除磷效率达到80％以上，磷回收达到30‑40％，为主流工艺自养脱氮耦合磷回收提供了新途径。
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| GB/T 7714 | 曾薇 , 李双双 , 苗豪豪 et al. 从生活污水中回收羟基磷灰石颗粒及自养脱氮的装置与方法 : CN202211358943.2[P]. | 2022-11-01 . |
| MLA | 曾薇 et al. "从生活污水中回收羟基磷灰石颗粒及自养脱氮的装置与方法" : CN202211358943.2. | 2022-11-01 . |
| APA | 曾薇 , 李双双 , 苗豪豪 , 彭永臻 . 从生活污水中回收羟基磷灰石颗粒及自养脱氮的装置与方法 : CN202211358943.2. | 2022-11-01 . |
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Abstract ：
Anaerobic ammonia oxidation (Anammox) coupling sulfur autotrophic denitrification is an effective method for the advanced nitrogen removal from the wastewater with limited carbon source. The influence of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification was investigated by adding different concentrations of S2O32--S (0, 39, 78, 156 and 312 mg/L) to the Anammox system. The contribution of sulfur autotrophic denitrification and Anammox to nitrogen removal at S2O32--S concentrations of 156 mg/L was 75% similar to 83% and 17%similar to 25%, respectively, and the mixed system achieved completely nitrogen removal. However, Anammox bioactivity was completely inhibited at S2O32--S concentrations up to 312 mg/L, and only sulfur autotrophic denitrification occurred. The isotopic effects of NO2--N (delta N-15(NO2) and delta O-18(NO2)) and NO3--N (delta N-15(NO3) and delta O-18(NO3)) during Anammox coupling sulfur autotrophic denitrification showed a gradual decrease trend with the increase of S2O32--S addition. The ratios of delta N-15(NO2):delta O-18(NO2) and delta N-15(NO3):delta O-18(NO3) was maintained at 1.30-2.41 and 1.36-2.52, respectively, which revealed that Anammox was dominant nitrogen removal pathway at S2O32--S concentrations less than 156 mg/L. Microbial diversity gradually decreased with the increase of S2O32--S. The S2O32--S addition enhanced the S2O32--driven autotrophic denitrification and weakened the Anammox, leading to a gradually decreasing trend of the proportion of Candidatus Brocadia as Anammox bacteria from the initial 27% to 4% (S2O32--S of 156 mg/L). Yet Norank-f-Hydrogenophilaceae (more than 50%) and Thiobacillus (54%) as functional bacteria of autotrophic denitrification obviously increased. The appropriate amount of S2O32--S addition promoted the performance of Anammox coupling sulfur autotrophic denitrification achieved completely nitrogen removal.
Keyword ：
S2O32--driven autotrophic denitrification S2O32--driven autotrophic denitrification Isotope effect Isotope effect Anammox Anammox Complete nitrogen removal Complete nitrogen removal Microbial diversity Microbial diversity
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| GB/T 7714 | Liu, Hong , Zeng, Wei , Li, Jianmin et al. Effect of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects [J]. | WATER RESEARCH , 2022 , 218 . |
| MLA | Liu, Hong et al. "Effect of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects" . | WATER RESEARCH 218 (2022) . |
| APA | Liu, Hong , Zeng, Wei , Li, Jianmin , Zhan, Mengjia , Fan, Zhiwei , Peng, Yongzhen . Effect of S2O32--S addition on Anammox coupling sulfur autotrophic denitrification and mechanism analysis using N and O dual isotope effects . | WATER RESEARCH , 2022 , 218 . |
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Abstract ：
Simultaneous partial nitrification, biological phosphorous removal and sludge fermentation in continuous-flow system faced many challenges in wastewater treatment plants (WWTPs). In this study, a novel process was developed to achieve partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system dominated by Tetrasphaera treating low carbon/nitrogen ratio (COD/N) real domestic wastewater. After 80 days of operation, no external carbon was added into the influent. Nitrite accumulation ratio (NAR) reached 99.4% and removal efficiency of PO43- -P was 100%. The effluent concentration of total inorganic nitrogen (TIN) was lower than 2 mg/L. Compared with traditional biological nitrogen and phosphorus removal processes, the daily sludge discharge in continuous-flow PNPRF decreased by 61.9%. DNA concentration of the sludge supernatant after anaerobic zone was 2.26-fold of that before anaerobic zone, and percentages of intact cells decreased by 6.9% and necrotic cells increased by 8.3%, indicating that cell lysis caused the reduction of sludge discharge. The negative effect of prolonged anaerobic phase on transcriptional response of nxrB gene was more significant than that of amoA and hao gene, which resulted in stable partial nitrification. The activities of ammonium-oxidizing bacteria (AOB) were higher than that of nitrite-oxidizing bacteria (NOB), and reverse transcriptional PCR (RT-PCR) showed that RNA expression of AOB was higher than that of NOB. Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation in continuous-flow system dominated by Tetrasphaera solved the problems of carbon source deficiency and large amount of sludge discharge in treating real domestic wastewater process.
Keyword ：
Transcriptional level Transcriptional level Partial nitrification Partial nitrification Enhanced biological phosphorus removal Enhanced biological phosphorus removal Real domestic wastewater Real domestic wastewater Continuous flow Continuous flow
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| GB/T 7714 | Fan, Zhiwei , Zeng, Wei , Meng, Qingan et al. Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system and mechanism analysis at transcriptional level [J]. | CHEMICAL ENGINEERING JOURNAL , 2022 , 428 . |
| MLA | Fan, Zhiwei et al. "Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system and mechanism analysis at transcriptional level" . | CHEMICAL ENGINEERING JOURNAL 428 (2022) . |
| APA | Fan, Zhiwei , Zeng, Wei , Meng, Qingan , Liu, Hong , Ma, Chenyang , Peng, Yongzhen . Achieving partial nitrification, enhanced biological phosphorus removal and in-situ fermentation (PNPRF) in continuous-flow system and mechanism analysis at transcriptional level . | CHEMICAL ENGINEERING JOURNAL , 2022 , 428 . |
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Abstract ：
Titanium salt coagulant, as a new type of water treatment agent, has been widely studied, but most researches do not consider its effect on the biological treatment. In this study, different doses of TiCl4 were added to the biological phosphorus removal (BPR) system to investigate the impact of TiCl4 on BPR. The results showed that the addition of TiCl4 not only significantly reduced the phosphorus concentration in effluent (below 0.5 mg/L), but also kept it stable. Moreover, the sedimentation performance of activated sludge was improved, which was superior to the control group. According to the results of flow cytometry (FCM), a small amount of TiCl4 significantly improved the bioactivities, but excessive dosage caused inhibition. When the dosage of TiCl4 below 20 mg/L, polyphosphate accumulating metabolism (PAM) was strengthened. In addition, the richness of microbial community and the relative abundance of Candidatus Accumulibacter clades also increased. However, when the dosage reached 60 mg/L, the relative abundance of Candidatus Competibacter increased and the BPR system was deteriorated. This study suggests that the addition of appropriate concentration of TiCl4 can realize the synergistic enhancement of biological and chemical phosphorus removal in sewage treatment.
Keyword ：
Bacterial activity Bacterial activity Microbial community Microbial community TiCl4 TiCl4 Simultaneous chemical phosphorus removal (SCPR) Simultaneous chemical phosphorus removal (SCPR)
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| GB/T 7714 | Jia, Yuan , Zeng, Wei , Fan, Zhiwei et al. An effective titanium salt dosing strategy for phosphorus removal from wastewater: Synergistic enhancement of chemical and biological treatment [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
| MLA | Jia, Yuan et al. "An effective titanium salt dosing strategy for phosphorus removal from wastewater: Synergistic enhancement of chemical and biological treatment" . | SCIENCE OF THE TOTAL ENVIRONMENT 842 (2022) . |
| APA | Jia, Yuan , Zeng, Wei , Fan, Zhiwei , Meng, Qingan , Liu, Hongjun , Peng, Yongzhen . An effective titanium salt dosing strategy for phosphorus removal from wastewater: Synergistic enhancement of chemical and biological treatment . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 842 . |
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Abstract ：
Different from anammox, the combination of Fe (III) reduction coupled to anaerobic ammonium oxidation (Feammox) and nitrate/nitrite dependent ferrous oxidation (NDFO) do not require to control nitrite accumulation. Furthermore, sponge iron can avoid continuous iron supplementation in practice and is a good iron source for the occurrence of Feammox and NDFO in wastewater treatment. Therefore, a biofilter using sponge iron as carrier treating low nitrogen wastewater was built. In this study, the performances of nitrogen removal were explored under different hydraulic retention times (HRT) and gas-water ratios in sponge iron biofilter. And the pathways of nitrogen removal were analyzed by activity tests. The results showed ammonia removal efficiency reached 94.1% and total inorganic nitrogen removal efficiency was up to 70.6% at HRT of 19 h and gas-water ratio of 18. Compared to nitrogen removal by adsorption under non-aeration, the activity tests showed that total inorganic nitrogen loss was caused by Feammox and NDFO after aeration. The results of microbial communities showed that appearances of nitrifier-Nitrosomonadaceae, Feammox bacteria-Clostridiaceae and NDFO bacteria-Gallionellaceae resulted in deep nitrogen removal after aeration, in which Nitrosomonadaceae and Clostridiaceae contributed to ammonia removal and Gallionellaceae contributed to nitrite/nitrate reduction to nitrogen gas. Therefore, it was feasible to achieve deep autotrophic nitrogen removal and Fe (II) and Fe (III) cycle in sponge iron biofilter.
Keyword ：
NDFO NDFO Microbial communities Microbial communities Feammox Feammox Ammonia removal Ammonia removal Sponge iron biofilter Sponge iron biofilter
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| GB/T 7714 | Li, Jianmin , Zeng, Wei , Liu, Hong et al. Achieving deep autotrophic nitrogen removal in aerated biofilter driven by sponge iron: Performance and mechanism [J]. | ENVIRONMENTAL RESEARCH , 2022 , 213 . |
| MLA | Li, Jianmin et al. "Achieving deep autotrophic nitrogen removal in aerated biofilter driven by sponge iron: Performance and mechanism" . | ENVIRONMENTAL RESEARCH 213 (2022) . |
| APA | Li, Jianmin , Zeng, Wei , Liu, Hong , Zhan, Mengjia , Miao, HaoHao . Achieving deep autotrophic nitrogen removal in aerated biofilter driven by sponge iron: Performance and mechanism . | ENVIRONMENTAL RESEARCH , 2022 , 213 . |
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