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学者姓名:高景峰

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Deciphering the coupling of partial nitrification/anammox and sulfur autotrophic denitrification: Microbial metabolism and antibiotic resistance genes propagation SCIE
期刊论文 | 2023 , 452 | CHEMICAL ENGINEERING JOURNAL
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Abstract :

Partial nitrification/anaerobic ammonium oxidation (anammox) (PN/A) was considered as an economically beneficial and environmentally friendly biological nitrogen removal process. The elimination of nitrate produced by PN/A system was the key to improving the total nitrogen removal efficiency (TNRE) of the system under mainstream conditions. In this study, the feasibility of the coupling S(0)-driven autotrophic denitrification (SAD) with PN/A in a single sequencing moving bed biofilm reactor (SMBBR) at room temperature was explored. Additionally, biological treatment process is ideal environments for the emerge and spread of emerging pol-lutants antibiotic resistance genes (ARGs), therefore, the shifts of ARGs in the system were investigated. After the coupling of PN/A and SAD, SMBBR was steadily operated with high TNRE. Nitrite-oxidizing bacteria (Nitrospira and Candidatus Nitrotoga) and nitrite oxidoreductase enzyme were inhibited by SAD process. And anammox bacteria were enriched, especially oligotype GAGTTTAAT of Ca. Brocadia. Sulfurimonas became the predominant autotrophic denitrifier. Heterotrophic denitrifiers Thauera and Dechloromonas were elutriated out from the sys-tem during the coupling of PN/A and SAD process, and they might be potential hosts for ARGs, leading to the decrease of ARGs. Overall, this study proposed a new strategy that SAD could promote the TNRE of PN/A and cut the risks of ARGs propagation, and PN/A-SAD coupling system was a biological nitrogen removal technology with promising applications under mainstream conditions.

Keyword :

Single sequencing moving bed biofilm reactor Single sequencing moving bed biofilm reactor Oligotypes Oligotypes anammox anammox Antibiotic resistance genes Antibiotic resistance genes Partial nitrification Partial nitrification S(0) -driven autotrophic denitrification S(0) -driven autotrophic denitrification

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GB/T 7714 Wang, Zhiqi , Gao, Jingfeng , Zhao, Yifan et al. Deciphering the coupling of partial nitrification/anammox and sulfur autotrophic denitrification: Microbial metabolism and antibiotic resistance genes propagation [J]. | CHEMICAL ENGINEERING JOURNAL , 2023 , 452 .
MLA Wang, Zhiqi et al. "Deciphering the coupling of partial nitrification/anammox and sulfur autotrophic denitrification: Microbial metabolism and antibiotic resistance genes propagation" . | CHEMICAL ENGINEERING JOURNAL 452 (2023) .
APA Wang, Zhiqi , Gao, Jingfeng , Zhao, Yifan , Zhang, Yi , Yuan, Yukun , Dai, Huihui et al. Deciphering the coupling of partial nitrification/anammox and sulfur autotrophic denitrification: Microbial metabolism and antibiotic resistance genes propagation . | CHEMICAL ENGINEERING JOURNAL , 2023 , 452 .
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Different acesulfame potassium fate and antibiotic resistance propagation pattern in nitrifying and denitrifying sludge systems SCIE
期刊论文 | 2023 , 856 | SCIENCE OF THE TOTAL ENVIRONMENT
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Abstract :

Acesulfame potassium (ACE-K) is a widely utilized sugar substitute with increasing demand, which is frequently de-tected in various environmental matrix due to recalcitrance. However, a general consensus on the contribution of ni-trifying and denitrifying process to ACE-K removal is lacking. Therefore, ACE-K removal, its effects on antibiotic resistant genes (ARGs) propagation and microbial community in nitrifying sequencing batch reactor (N-SBR) and denitrifying sequencing batch reactor (D-SBR) inoculated with the identical activated sludge were investigated. In this study, ACE-K can be eliminated in N-SBR with satisfying removal efficiency (96.76 +/- 8.33 %) after 13 d acclima-tion, while it remained persistent (average ACE-K removal efficiency of 2.24 +/- 1.86 %) in D-SBR during 84 d expo-sure. Moreover, ACE-K hardly affected the performances of these two types of reactors and had little impact on nitrifying and denitrifying functional genes. However, initial contact with ACE-K would increase ARGs abundance, network analysis showed functional bacteria in each reactor were possible ARGs hosts. Potential ACE-K degrading gen-era Chelatococcus, Bosea and Aquamicrobium were found in both reactors. LefSe analysis showed that Phyllobacteriaceae containing Aquamicrobium genus was a differentially enriched family in N-SBR. This research might provide a perspec-tive for better understanding factor affecting ACE-K fate in wastewater treatment process and its ecological risks.

Keyword :

Acesulfame potassium Acesulfame potassium Biodegradation Biodegradation Antibiotic resistance genes Antibiotic resistance genes Nitrifying and denitrifying sludge Nitrifying and denitrifying sludge

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GB/T 7714 Li, Ziqiao , Gao, Jingfeng , Zhao, Yifan et al. Different acesulfame potassium fate and antibiotic resistance propagation pattern in nitrifying and denitrifying sludge systems [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 856 .
MLA Li, Ziqiao et al. "Different acesulfame potassium fate and antibiotic resistance propagation pattern in nitrifying and denitrifying sludge systems" . | SCIENCE OF THE TOTAL ENVIRONMENT 856 (2023) .
APA Li, Ziqiao , Gao, Jingfeng , Zhao, Yifan , Wang, Zhiqi , Cui, Yingchao , Li, Dingchang et al. Different acesulfame potassium fate and antibiotic resistance propagation pattern in nitrifying and denitrifying sludge systems . | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 856 .
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Short-term stress of quaternary ammonium compounds on intracellular and extracellular resistance genes in denitrification systems SCIE
期刊论文 | 2023 , 452 | CHEMICAL ENGINEERING JOURNAL
WoS CC Cited Count: 3
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Abstract :

The wastewater containing high quaternary ammonium compounds (QACs) concentrations from hospitals and other sources may cause the transient shock loads on the wastewater treatment plants (WWTPs). Denitrification is the key part of nitrogen removal from wastewater, however, the effects of QACs on the denitrification and the spread patterns of intracellular and extracellular resistance genes were still unknown. The short-term stress of three frequently detected QACs on denitrification were systematically investigated in this study. The inhibitory effect on nitrate removal had the following rules: BAC C12 > ATMAC C12 > DADMAC C12. The inhibition of QACs on denitrification performance was correlated with the reduction of Thauera abundance, and a higher QACs concentration made stronger inhibitory effect. Moreover, high concentrations of QACs could cause a significant decrease in reactive oxygen species, inhibit cell activity and extracellular polymeric substances secretion. Furthermore, QACs stimulated an overall increase of intracellular and extracellular QACs resistance genes in denitrification systems, both in sludge and in water. And co-selection among resistance genes occurred under shock loads of QACs, especially a high BAC C12 concentration caused a substantial raise in abundances of resistance genes in water. It was worth noting that resistance genes in sludge and water were mainly contributed by different bacteria. Rhizobium and Pseudomonas may be the potential hosts contributed to resistance genes in sludge while Thauera mainly contributed to that in water.

Keyword :

Intracellular and extracellular resistance genes Intracellular and extracellular resistance genes Potential hosts Potential hosts Quaternary ammonium compounds Quaternary ammonium compounds Denitrification Denitrification Microbial community Microbial community

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GB/T 7714 Zhao, Mingyan , Gao, Jingfeng , Liu, Ying et al. Short-term stress of quaternary ammonium compounds on intracellular and extracellular resistance genes in denitrification systems [J]. | CHEMICAL ENGINEERING JOURNAL , 2023 , 452 .
MLA Zhao, Mingyan et al. "Short-term stress of quaternary ammonium compounds on intracellular and extracellular resistance genes in denitrification systems" . | CHEMICAL ENGINEERING JOURNAL 452 (2023) .
APA Zhao, Mingyan , Gao, Jingfeng , Liu, Ying , Wang, Zhiqi , Wu, Zejie , Zhang, Haoran et al. Short-term stress of quaternary ammonium compounds on intracellular and extracellular resistance genes in denitrification systems . | CHEMICAL ENGINEERING JOURNAL , 2023 , 452 .
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Inhibition of Nitrospira and Nitrotoga by paracetamol achieved the rapid start-up and long-term stable operation of partial nitrification for low-strength ammonium wastewater SCIE
期刊论文 | 2023 , 454 | CHEMICAL ENGINEERING JOURNAL
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Abstract :

Achieving stable partial nitrification (PN) are the basic guarantee of anaerobic ammonia oxidation (anammox) process. However, there is no recognized feasible method for rapidly achieving stable PN in municipal wastewater, and further research is necessary. In this study, paracetamol was proved as an effective nitrite oxidation inhibitor through long-term experiment lasting 392 days in three systems (floc sludge, granular sludge, biofilm). PN of low-strength ammonium wastewater was rapidly achieved within 28 days and maintained for about 80 days after paracetamol dose, whereas PN recovered again within 10 days and sustained over 150 days under paracetamol dose combined with low dissolved oxygen strategy. The inhibition effect of paracetamol on NOB was almost not affected by biomass growth modes. QPCR and high-throughput sequencing showed that Nitrospira (Oligotype 1) was inhibited by paracetamol, and PICURSt2 analysis indicated its key enzyme NXR was significantly downregulated after paracetamol treatment, which were the key reasons of PN start-up in three systems. The emergence and adaptation of Nitrotoga was the main reason for the instability of PN. This study proved that paracetamol could be used as a novel nitrite oxidation inhibitor, and provided a possibility to achieve the mainstream PN and anammox for treating wastewater with low-strength ammonium.

Keyword :

Low-strength ammonium wastewater Low-strength ammonium wastewater Bacterial community structure Bacterial community structure Biomass growth mode Biomass growth mode Paracetamol Paracetamol Partial nitrification Partial nitrification

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GB/T 7714 Cui, Yingchao , Gao, Jingfeng , Wu, Zejie et al. Inhibition of Nitrospira and Nitrotoga by paracetamol achieved the rapid start-up and long-term stable operation of partial nitrification for low-strength ammonium wastewater [J]. | CHEMICAL ENGINEERING JOURNAL , 2023 , 454 .
MLA Cui, Yingchao et al. "Inhibition of Nitrospira and Nitrotoga by paracetamol achieved the rapid start-up and long-term stable operation of partial nitrification for low-strength ammonium wastewater" . | CHEMICAL ENGINEERING JOURNAL 454 (2023) .
APA Cui, Yingchao , Gao, Jingfeng , Wu, Zejie , Wang, Zhiqi , Zhao, Yifan , Guo, Yi . Inhibition of Nitrospira and Nitrotoga by paracetamol achieved the rapid start-up and long-term stable operation of partial nitrification for low-strength ammonium wastewater . | CHEMICAL ENGINEERING JOURNAL , 2023 , 454 .
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Quaternary ammonium compounds promoted anoxic sludge granulation and altered propagation risk of intracellular and extracellular antibiotic resistance genes SCIE
期刊论文 | 2023 , 445 | JOURNAL OF HAZARDOUS MATERIALS
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Abstract :

Surfactants could influence sludge morphology and disinfectants were linked to antibiotic resistance genes (ARGs). Thus, the response of activated sludge and ARGs to long-term quaternary ammonium compounds (QACs) exposure required further investigation, which is a popular surfactant and disinfectant. Here, three sequencing batch reactors were fed with 5 mg/L most frequently detected QACs (dodecyl trimethyl ammonium chloride (ATMAC C12), dodecyl benzyl dimethyl ammonium chloride (BAC C12) and didodecyl dimethyl ammonium chloride (DADMAC C12)) for 180 d. The long-term inhibitory effect on denitrification ranked: DADMAC C12 > BAC C12 > ATMAC C12. Besides, obvious granular sludge promoted by the increase of alpha-Helix/(8-Sheet + Random coil) appeared in DADMAC C12 system. Moreover, intracellular ARGs increased when denitrification systems encountered QACs acutely but decreased in systems chronically exposed to QACs. Although replication and repair metabolism in ATMAC C12 system was higher, ATMAC C12 significantly promoted proliferation of extracellular ARGs. It was noteworthy that the propagation risk of extracellular ARGs in sludge increased significantly during sludge granulation process, and intracellular sul2 genes in sludge and water both increased with the granular diameter in DADMAC C12 system. The universal utilization of QACs may enhance antibiotic resistance of bacteria in wastewater treatment plants, deserving more attention.

Keyword :

Intracellular and extracellular antibiotic&nbsp Intracellular and extracellular antibiotic&nbsp Denitrification system Denitrification system Bacterial community structure Bacterial community structure resistance genes resistance genes Sludge granulation Sludge granulation Quaternary ammonium compounds Quaternary ammonium compounds

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GB/T 7714 Zhao, Mingyan , Gao, Jingfeng , Zhang, Haoran et al. Quaternary ammonium compounds promoted anoxic sludge granulation and altered propagation risk of intracellular and extracellular antibiotic resistance genes [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 445 .
MLA Zhao, Mingyan et al. "Quaternary ammonium compounds promoted anoxic sludge granulation and altered propagation risk of intracellular and extracellular antibiotic resistance genes" . | JOURNAL OF HAZARDOUS MATERIALS 445 (2023) .
APA Zhao, Mingyan , Gao, Jingfeng , Zhang, Haoran , Cui, Yingchao , Wang, Zhiqi , Zhao, Yifan et al. Quaternary ammonium compounds promoted anoxic sludge granulation and altered propagation risk of intracellular and extracellular antibiotic resistance genes . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 445 .
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Mitigating the inhibition of antibacterial agent chloroxylenol on nitrification system-The role of Rhodococcus ruber in a bioaugmentation system SCIE
期刊论文 | 2023 , 447 | JOURNAL OF HAZARDOUS MATERIALS
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Abstract :

The chloroxylenol (PCMX) degrading strain was successfully isolated from sludge and identified as Rhodococcus ruber (R. ruber). Afterwards, a bioaugmentation system was constructed by seeding R. ruber into nitrifying sludge to fasten degradation efficiency of highly toxic PCMX from wastewater. Results showed that R. ruber presented high PCMX-degrading performance under aerobic conditions, 25 degrees C, pH 7.0 and inoculum sizes of 4% (v/v). These optimized conditions were used in subsequent bioaugmentation experiment. In bioaugmentation system, R. ruber could detoxify nitrifiers by degrading PCMX, and the content of polysaccharide in extracellular poly-meric substances increased. The quantitative polymerase chain reaction results exhibited that the absolute abundance of 16S rRNA gene and ammonia oxidizing bacteria (AOB) slightly elevated in bioaugmentation system. After analyzing the results of high-throughput sequencing, it was found that the loaded R. ruber can colonize successfully and turn into dominant strains in sludge system. Molecular docking simulation showed that PCMX had a weaker suppressed effect on AOB than nitrite oxidizing bacteria, and R. ruber can alleviate the adverse effect. This study could provide a novel strategy for potential application in reinforcement of PCMX removal in wastewater treatment.

Keyword :

Bacterial community Bacterial community Molecular docking simulation Molecular docking simulation Rhodococcus ruber Rhodococcus ruber Chloroxylenol Chloroxylenol Bioaugmentation Bioaugmentation

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GB/T 7714 Guo, Yi , Gao, Jingfeng , Zhao, Yifan et al. Mitigating the inhibition of antibacterial agent chloroxylenol on nitrification system-The role of Rhodococcus ruber in a bioaugmentation system [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 447 .
MLA Guo, Yi et al. "Mitigating the inhibition of antibacterial agent chloroxylenol on nitrification system-The role of Rhodococcus ruber in a bioaugmentation system" . | JOURNAL OF HAZARDOUS MATERIALS 447 (2023) .
APA Guo, Yi , Gao, Jingfeng , Zhao, Yifan , Liu, Ying , Zhao, Mingyan , Li, Ziqiao . Mitigating the inhibition of antibacterial agent chloroxylenol on nitrification system-The role of Rhodococcus ruber in a bioaugmentation system . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 447 .
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Partial S(0)-driven autotrophic denitrification process facilitated the quick natural enrichment of anammox bacteria at room temperature SCIE
期刊论文 | 2023 , 855 | SCIENCE OF THE TOTAL ENVIRONMENT
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Abstract :

Anaerobic ammonium oxidation (anammox) is well-known to be an environmental and promising biotechnology. However, the natural enrichment of anammox bacteria is still a challenging topic. In this study, partial S(0)-driven au-totrophic denitrification (PSAD) was developed to stably supply nitrite, and natural enrichment of anammox bacteria was rapidly realized in a single sequencing moving bed biofilm reactor at room temperature. With the initiation of PSAD, anammox bacteria spontaneously emerged within 12 days, and PSAD-anammox coupling system was realized successfully. And then, the influent concentration of ammonium continuously increased to the same concentration as the nitrate, and the mean total nitrogen removal efficiency reached 92.77 %, which was mainly contributed by anammox. Moreover, the coupling of PSAD and anammox reduced the risk of sulfate emissions. cDNA high throughput sequencing revealed that the relative abundance of Candidatus Brocadia and Thiobacillus reached 39.03 % and 13.48 % at the 88th day. Oligotyping analysis illustrated that GATTTAAT and GTCCCA were the dominant Ca. Brocadia and Thiobacillus oligotypes in PSAD-anammox coupling system, respectively. DNA-based stable isotope probing further deciphered that Thiobacillus was the actual performer of PSAD and supported the nitrite for anammox bacteria in PSAD-anammox coupling system. Overall, this work provided a new strategy to naturally enrich anammox bacteria.

Keyword :

Partial sulfur autotrophic denitrification Partial sulfur autotrophic denitrification cDNA high-throughput sequencing cDNA high-throughput sequencing Anaerobic ammonium oxidation Anaerobic ammonium oxidation Oligotypes Oligotypes DNA-based stable isotope probing DNA-based stable isotope probing

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GB/T 7714 Wang, Zhiqi , Gao, Jingfeng , Dai, Huihui et al. Partial S(0)-driven autotrophic denitrification process facilitated the quick natural enrichment of anammox bacteria at room temperature [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 855 .
MLA Wang, Zhiqi et al. "Partial S(0)-driven autotrophic denitrification process facilitated the quick natural enrichment of anammox bacteria at room temperature" . | SCIENCE OF THE TOTAL ENVIRONMENT 855 (2023) .
APA Wang, Zhiqi , Gao, Jingfeng , Dai, Huihui , Yuan, Yukun , Zhao, Yifan , Li, Dingchang et al. Partial S(0)-driven autotrophic denitrification process facilitated the quick natural enrichment of anammox bacteria at room temperature . | SCIENCE OF THE TOTAL ENVIRONMENT , 2023 , 855 .
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The spread of different resistance genes fractions in nitrification system under chronic exposure to varying alkyl chain length benzalkyl dimethylammonium compounds SCIE
期刊论文 | 2023 , 371 | BIORESOURCE TECHNOLOGY
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Abstract :

Benzalkyl dimethylammonium compounds (BACs) are generally applied as surfactants and disinfectants. In this study, the nitrification systems were exposed to different alkyl chain lengths (C12-C16) and different levels of BACs (0-5 mg/L), respectively, totally 120 days and to explore the chronic effect of BACs on resistance genes (RGs). RGs were classified into four fractions based on activated sludge properties. Ammonia oxidation per-formance were not significantly affected by BACs, whereas BACs increased the absolute abundance of most intracellular RGs in sludge (si-RGs). Under the exposure of BACs, extracellular RGs in water (we-RGs) showed a decrease trend and si-RGs tended to be converted to we-RGs. Tightly bound-Tyrosine side chain was significantly correlated with most we-RGs, and we-intI1 might contribute to the propagation of RGs. Therefore, the risk of transmission of different fractions of RGs in the nitrification system under the stress of BACs should be taken seriously.

Keyword :

Nitrification Nitrification Mobile genetic elements Mobile genetic elements Benzalkyl dimethylammonium compounds Benzalkyl dimethylammonium compounds Intracellular and extracellular resistance genes Intracellular and extracellular resistance genes Protein secondary structure Protein secondary structure

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GB/T 7714 Zhang, Haoran , Gao, Jingfeng , Zhao, Mingyan et al. The spread of different resistance genes fractions in nitrification system under chronic exposure to varying alkyl chain length benzalkyl dimethylammonium compounds [J]. | BIORESOURCE TECHNOLOGY , 2023 , 371 .
MLA Zhang, Haoran et al. "The spread of different resistance genes fractions in nitrification system under chronic exposure to varying alkyl chain length benzalkyl dimethylammonium compounds" . | BIORESOURCE TECHNOLOGY 371 (2023) .
APA Zhang, Haoran , Gao, Jingfeng , Zhao, Mingyan , Wang, Zhiqi , Li, Dingchang , Wu, Zejie et al. The spread of different resistance genes fractions in nitrification system under chronic exposure to varying alkyl chain length benzalkyl dimethylammonium compounds . | BIORESOURCE TECHNOLOGY , 2023 , 371 .
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Family Sphingomonadaceae as the key executor of triclosan degradation in both nitrification and denitrification systems SCIE
期刊论文 | 2022 , 442 | CHEMICAL ENGINEERING JOURNAL
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The usage of triclosan (TCS) has increased with the COVID-19 virus outbreak, causing more TCS are released into wastewater treatment systems. However, the difference in TCS removal pathway and TCS degrading bacteria between nitrification and denitrification systems was still unknown. In this study, batch tests of TCS biodegradation mechanism and DNA stable isotope probing (DNA-SIP) technique were applied to decrypt the different TCS removal pathway and the corresponding degrading taxon between two nitrification and two denitrification systems. The main TCS degradation pathway in both nitrification and denitrification systems were the metabolism of heterotrophic bacteria, only a little TCS was degraded by the co-metabolism of heterotrophic or nitrifying bacteria, and higher NH4+-N or NO3--N concentration contributed to more TCS degradation. Moreover, denitrification systems had stronger TCS removal capacity (0.11 and 0.65 mg TCS/g SS) than nitrification systems (0.83 and 1.12 mg TCS/g SS). DNA-SIP assay further revealed that active TCS degrading bacteria in both systems belonged to Sphingomonadaceae family. Furthermore, the oligotype TATGCC, TAATCA and GCCCCG of Sphingomonadaceae played important roles in degrading TCS in both systems. Moreover, reactor performance and mixed liquor suspended solids might play important roles in shaping the ecotypes of Sphingomonadaceae, which caused the difference in degrading TCS between nitrification and denitrification systems. This lab-scale research might provide meaningful opportunities for evaluating the scale-up applications, and the TCS degrading bacteria identified in present study might be recommended to be used as bioaugmentation strains in practical engineering.

Keyword :

DNA stable isotope probing DNA stable isotope probing Oligotyping Oligotyping Denitrification system Denitrification system Triclosan Triclosan Active triclosan-degrading bacteria Active triclosan-degrading bacteria Nitrification system Nitrification system

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GB/T 7714 Dai, Huihui , Gao, Jingfeng , Li, Dingchang et al. Family Sphingomonadaceae as the key executor of triclosan degradation in both nitrification and denitrification systems [J]. | CHEMICAL ENGINEERING JOURNAL , 2022 , 442 .
MLA Dai, Huihui et al. "Family Sphingomonadaceae as the key executor of triclosan degradation in both nitrification and denitrification systems" . | CHEMICAL ENGINEERING JOURNAL 442 (2022) .
APA Dai, Huihui , Gao, Jingfeng , Li, Dingchang , Wang, Zhiqi , Cui, Yingchao , Zhao, Yifan . Family Sphingomonadaceae as the key executor of triclosan degradation in both nitrification and denitrification systems . | CHEMICAL ENGINEERING JOURNAL , 2022 , 442 .
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Photocatalysis-enhanced coagulation for removal of intracellular organic matter from Microcystis aeruginosa: Efficiency and mechanism SCIE
期刊论文 | 2022 , 283 | SEPARATION AND PURIFICATION TECHNOLOGY
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Photocatalysis is an efficient advanced oxidation process that provides a promising alternative for pollution control during algal blooms in lakes or water reservoirs. Previous research has mainly focused on the destruction of algal cells by photocatalysis, whereas the release and control of concomitant secretions specially intracellular organic matter (IOM) is undervalued. Herein, we studied the removal efficiency and mechanism of algal IOM extracted from Microcystis aeruginosa during stationary phase by the combined process of pre-photocatalysis with a novel composite of Bi2O3-TiO2/PAC (Bi-doped TiO2 nano-composites supported by powdered activated carbon) followed by enhanced coagulation with aluminum sulfate (AS) coagulant. Optimized photocatalysis and coagulation combined treatment (PC) was indicated to enhance IOM removal with 64%, 92% and 100% for DOC, absorbance at 254 nm (UV254) and the optical density at 680 nm (OD680) respectively. Pre-photocatalysis reduced the required AS coagulant dose and weakened the effect of solution pH. Photocatalysis was more inclined to remove humic substances and building blocks than high-molecular weight components, while coagulation alone displayed preference for high-molecular weight biopolymers and protein-like substance. Pre-photocatalysis was complementary with subsequent coagulation for efficient removal of protein-like sub-stances and fractionation with medium molecular weight of 0.5-1 kDa. The produced flocs revealed that the Bi2O3-TiO2/PAC adsorbing IOM acted as a flocculation core to improve coagulation performance. Besides, the combined PC process was proved to perform well to co-existing algal pollution without damaging cells integrity and enhanced algal cells removal from 26% by coagulation alone to 61%. Therefore, this combined treatment was an effective method for the removal of IOM of Microcystis aeruginosa and control cyanobacteria bloom.

Keyword :

Intracellular organic matter Intracellular organic matter Pre-photocatalysis Pre-photocatalysis Bi2O3 -TiO2/PAC composite Bi2O3 -TiO2/PAC composite Enhanced coagulation Enhanced coagulation Microcystis aeruginosa Microcystis aeruginosa

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GB/T 7714 Wang, Huiping , Yang, Yanling , Zhou, Zhiwei et al. Photocatalysis-enhanced coagulation for removal of intracellular organic matter from Microcystis aeruginosa: Efficiency and mechanism [J]. | SEPARATION AND PURIFICATION TECHNOLOGY , 2022 , 283 .
MLA Wang, Huiping et al. "Photocatalysis-enhanced coagulation for removal of intracellular organic matter from Microcystis aeruginosa: Efficiency and mechanism" . | SEPARATION AND PURIFICATION TECHNOLOGY 283 (2022) .
APA Wang, Huiping , Yang, Yanling , Zhou, Zhiwei , Li, Xing , Gao, Jingfeng , Yu, Rui et al. Photocatalysis-enhanced coagulation for removal of intracellular organic matter from Microcystis aeruginosa: Efficiency and mechanism . | SEPARATION AND PURIFICATION TECHNOLOGY , 2022 , 283 .
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