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学者姓名:崔有为
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Abstract :
Although heterotrophic nitrification and aerobic denitrification (HN-AD) bacteria, a novel functional group involved in nitrogen conversion, have been isolated and characterized, the lack of specific molecular markers for identification severely limits the study of their role in geochemical cycling and the contribution in ecosystems. Here, a set of molecular markers was developed for the rapid identification of HN-AD bacteria, via delving into the genomics and transcriptomics of a HN-AD isolate (Pseudomonas aeruginosa SNDPR-01). Among the nine candidate genes that were significantly expressed during heterotrophic nitrification, three were involved in the conversion of hydroxylamine to nitrite, a characteristic process of HN-AD. The universality and stability of the identification methods based on the gene primer set were validated using pure HN-AD strains, mixed cultures of pure HN-AD strains, and activated sludge from laboratory-scale and real wastewater treatment plants. In all cases, the amplification outcome was positively correlated with the function and population of HN-AD bacteria, demonstrating its validity as a molecular marker. This study supports the paradigm of heterotrophic nitrification from hydroxylamine to nitrite. As an effective tool for the identification of classic HN-AD bacteria, this study lays the groundwork for research on environmental ecology and biotechnological application of HN-AD bacteria.
Keyword :
Primer Primer Heterotrophic nitrification and aerobic Heterotrophic nitrification and aerobic Molecular marker Molecular marker Gene cloning Gene cloning denitrification denitrification Transcriptome Transcriptome
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GB/T 7714 | Yang, Rui-chun , Cui, You-wei , Li, Zhen-ying et al. Molecular identification of heterotrophic nitrification and aerobic denitrification bacteria: From methods development to application demonstration [J]. | WATER RESEARCH , 2025 , 280 . |
MLA | Yang, Rui-chun et al. "Molecular identification of heterotrophic nitrification and aerobic denitrification bacteria: From methods development to application demonstration" . | WATER RESEARCH 280 (2025) . |
APA | Yang, Rui-chun , Cui, You-wei , Li, Zhen-ying , Li, Ming-teng , Jiang, Liu-xu , Mi, Ya-nan et al. Molecular identification of heterotrophic nitrification and aerobic denitrification bacteria: From methods development to application demonstration . | WATER RESEARCH , 2025 , 280 . |
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Abstract :
The pharmaceutical industry, an essential sector of the global economy, heavily relies on ethanol solvents, which leads to significant volatile organic compounds (VOCs) emissions. As a sustainable treatment method aligning with carbon reduction goals, this study proposed and demonstrated a synergistic approach of using microalgae (Chlorella sorokiniana FACHB-24) and acetic acid bacteria (Acetobacter pasteurianus CICC 20056) to recover ethanol into value-added products (algal lipids). In the innovative co-culture, A. pasteurianus oxidizes ethanol to acetic acid, which is fed to algae for lipid production. This method increased biomass and lipid yield by 21.29% and 150.16% (p < 0.05), respectively, compared to microalgae directly using ethanol. Some operational parameters including ethanol concentration, bacterial-algal biomass ratio, pH value, and light intensity made influence on lipid production. Under the optimal conditions (1.0% v/v ethanol concentration, 1:10 bacterial-algal biomass ratio, pH 6.5, and 5000 lux light intensity), the maximal biomass and lipid yields were 572.5 mg L- 1 and 161.1 mg L- 1 (26.7% lipid content), respectively. In the harvested lipid from microalgae, C16 - C18 fatty acids made up 98.22% of the total fatty acid methyl esters content. In proteomic comparison of the single culture and co-culture, the conversion of ethanol to acetate by A. pasteurianus provides C. sorokiniana with a more efficient acetyl-CoA source by bypassing energy-intensive glycolysis and directly enhancing lipid synthesis. This study provides a solution to increasing the lipid production from ethanol gas as a sustainable VOCs management of pharmaceutical industry.
Keyword :
Lipid production Lipid production Hydrophilic VOCs Hydrophilic VOCs Synergistic interaction Synergistic interaction Proteomics Proteomics Symbiotic bacterial-algal consortium Symbiotic bacterial-algal consortium
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GB/T 7714 | Jiang, Liu-Xu , Cui, You-Wei , Mi, Ya-Nan et al. Recovery of volatile ethanol gas via microalgal-bacterial consortium: Ethanol-to-acetate conversion pathway boosts lipid production [J]. | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2025 , 381 . |
MLA | Jiang, Liu-Xu et al. "Recovery of volatile ethanol gas via microalgal-bacterial consortium: Ethanol-to-acetate conversion pathway boosts lipid production" . | JOURNAL OF ENVIRONMENTAL MANAGEMENT 381 (2025) . |
APA | Jiang, Liu-Xu , Cui, You-Wei , Mi, Ya-Nan , Zhou, Dong-Xu , Li, Ming-Teng , Yang, Rui-Chun . Recovery of volatile ethanol gas via microalgal-bacterial consortium: Ethanol-to-acetate conversion pathway boosts lipid production . | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2025 , 381 . |
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Abstract :
The overgrowth of filamentous fungi leading to sludge bulking constitutes a significant threat to the structural and functional stability of aerobic granular sludge (AGS). This study investigated the inhibitory effects of magnetic powder (Fe3O4) on filamentous fungi, analyzed its toxic mechanism, and evaluated its effectiveness in mitigating sludge bulking. Results indicated that the inhibitory effects of Fe3O4 were closely related to its concentration and particle size. Different concentrations and particle sizes of Fe3O4 led to a regular decrease in both fungi and bacteria populations, with a significant reduction in fungi population. The relative abundance of filamentous fungi decreased from 98.01 % (0 g/L Fe3O4) to 0.32 %-7.19 % (1.0 g/L Fe3O4), indicating the toxicity of Fe3O4 in both fungi and bacteria. The toxic mechanism was attributed to the effects of Fe3O4 on the microbial interface. The change in sludge surface properties promoted the physical penetration of Fe3O4, resulting in oxidative stress and membrane damage of microorganisms. Filamentous fungi were more susceptible to toxicity than bacteria due to their larger specific surface area and extracellular polymeric substance coating, which facilitated adhesive contact with Fe3O4, providing more points for penetration. This study explored how Fe3O4 inhibits filamentous fungi in the mixed fungi-bacteria systems, proposed a conceptual model for the action of Fe3O4 on filamentous fungi surface, and established an effective method to restore sludge bulking, which provided a solution to the AGS disaggregated problem caused by filamentous fungal overgrowth.
Keyword :
Toxicity Toxicity Interface effects Interface effects Magnetic bio-effects Magnetic bio-effects Magnetic powder (Fe 3 O 4 ) Magnetic powder (Fe 3 O 4 ) Filamentous fungi Filamentous fungi
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GB/T 7714 | Zhou, Dong-Xu , Cui, You-Wei , Mi, Ya-Nan et al. Inhibiting overgrowth of filamentous fungi by magnetic powders: Combination effects of particle interface toxicity and magnetic microbe [J]. | CHEMICAL ENGINEERING JOURNAL , 2025 , 506 . |
MLA | Zhou, Dong-Xu et al. "Inhibiting overgrowth of filamentous fungi by magnetic powders: Combination effects of particle interface toxicity and magnetic microbe" . | CHEMICAL ENGINEERING JOURNAL 506 (2025) . |
APA | Zhou, Dong-Xu , Cui, You-Wei , Mi, Ya-Nan , Jiang, Liu-Xu , Li, Ming-Teng , Liang, Hui-Kai et al. Inhibiting overgrowth of filamentous fungi by magnetic powders: Combination effects of particle interface toxicity and magnetic microbe . | CHEMICAL ENGINEERING JOURNAL , 2025 , 506 . |
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Abstract :
Efficient antibiotics removal lowers the transmission risk of antibiotic resistance genes (ARGs). However, low efficiency limits the application of biological methods for antibiotics removal. Herein, a mixed culture of halotolerant fungi-bacteria was used for treatment of saline wastewater containing tetracycline (TC). Furthermore, static magnetic field (SMF) was used to increase TC removal. The study examined the effectiveness of SMF in removing antibiotics from saline wastewater and the associated risk of ARGs transmission. The results demonstrated that the application of a 40 mT SMF significantly improved the TC removal efficiency by 37.09 %, compared to the control (SMF=0) The TC was mainly removed through biodegradation and adsorption. In biodegradation, SMF enhanced electron transport system activity, and activities of lignin-degrading enzymes which led to higher TC biodegradation. The activity of lactate dehydrogenase and malondialdehyde decreased, lowering the damage of microbial cell membranes by TC. During the adsorption process, higher generation of extracellular polymeric substances was observed under SMF, which caused an increase in TC removal via adsorption. Microbial community analysis revealed that SMF facilitated the enrichment of TC-degrading microorganisms. Under SMF, vertical gene transfer of ARGs increased, while horizontal gene transfer risk decreased
Keyword :
Tetracycline Tetracycline Saline antibiotic wastewater Saline antibiotic wastewater Antibiotic resistance genes Antibiotic resistance genes Static magnetic fields Static magnetic fields
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GB/T 7714 | Li, Zhen-Ying , Cui, You-Wei , Liang, Hui-Kai et al. Tetracycline degradation by a mixed culture of halotolerant fungi-bacteria under static magnetic field: Mechanism and antibiotic resistance genes transfer [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2025 , 492 . |
MLA | Li, Zhen-Ying et al. "Tetracycline degradation by a mixed culture of halotolerant fungi-bacteria under static magnetic field: Mechanism and antibiotic resistance genes transfer" . | JOURNAL OF HAZARDOUS MATERIALS 492 (2025) . |
APA | Li, Zhen-Ying , Cui, You-Wei , Liang, Hui-Kai , Yan, Hui-Juan , Yang, Rui-Chun . Tetracycline degradation by a mixed culture of halotolerant fungi-bacteria under static magnetic field: Mechanism and antibiotic resistance genes transfer . | JOURNAL OF HAZARDOUS MATERIALS , 2025 , 492 . |
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Abstract :
The pharmaceutical industry is a significant emission source of water-soluble volatile organic compounds (VOCs). However, these VOCs are primarily treated through adsorption on activated carbon or combustion methods, leading to high the generation of hazardous solid waste or energy consumption. This study proposed an innovative approach to address these issues by integrating spray absorption with biological treatment in sequencing batch reactors (SBRs). The results indicated that after spray absorption, more than 90 % of ethanol could be removed in SBRs, while 1-6 % of ethanol was emitted into the atmosphere, depending on aeration intensity and ethanol concentration. Although the intermittent emissions of ethanol gas resulted in a high variation in loading into the activated sludge system, stable and efficient treatment of ethanol was achieved. Amaricoccus was predominated as the primary bacterium responsible for ethanol degradation, indicating a robust microbial community capable of adapting to fluctuating ethanol levels. This study provides an environment-friendly and efficient solution to treating water-soluble VOCs. The proposed method utilizes existing infrastructure such as spray towers and biological treatment facilities commonly found in pharmaceutical enterprises, making it a cost-effective and easily implementable technology. This study contributes to the advancement of understanding in the field of VOCs treatment.
Keyword :
Volatile ethanol gas Volatile ethanol gas Spraying absorption Spraying absorption Ethanol shock loads Ethanol shock loads Biological treatment Biological treatment Ethanol emissions Ethanol emissions
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GB/T 7714 | Zhu, Wen-Jie , Cui, You-Wei , Yang, Hou-Jian . Treatment of volatile ethanol gas emitted by pharmaceutical manufacturing industry in a combination process of spraying absorption and wastewater biological treatment [J]. | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2024 , 12 (6) . |
MLA | Zhu, Wen-Jie et al. "Treatment of volatile ethanol gas emitted by pharmaceutical manufacturing industry in a combination process of spraying absorption and wastewater biological treatment" . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING 12 . 6 (2024) . |
APA | Zhu, Wen-Jie , Cui, You-Wei , Yang, Hou-Jian . Treatment of volatile ethanol gas emitted by pharmaceutical manufacturing industry in a combination process of spraying absorption and wastewater biological treatment . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2024 , 12 (6) . |
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Abstract :
There is very limited research on the application of moderate halophiles for biotreatment of hypersaline wastewater widely generated from some industries. This study demonstrated the development of moderate halophiles inoculated from saltern sediments into aerobic granule sludge (AGS) to treat hypersaline wastewater with a salinity of 100 g/L. The granulation of moderate halophiles can occur without applying the settling ve-locity selective pressure. The saltern sediment initially aggregated into single small granules and finally devel-oped into 1200 +/- 50 mu m multiparticle granules. The halophiles affiliated in Halomonas was dominant in the granular bacterial community, with a relative abundance of 94.52%. Halomonas ventosae secreted sulfated polysaccharides. The sulfated polysaccharides content accounted for 63.95 +/- 2.10% in the polysaccharides (PS), having an adhesive role in connecting single granules. Multiparticle granules showed the clear stratified struc-ture, with alpha-D-glucopyranose polysaccharides in the inner bounders and beta-D-glucopyranose polysaccharides in the outer. The moderately granular sludge showed the stable chemical oxygen demand (COD) removal efficiency of >90% and the aerobic total inorganic nitrogen (TIN) removal efficiency (equal to ammonia removal) of 70 +/- 5.00%. This paper contributes new insight into the formation of moderately halophilic granular sludge and accelerates the application of moderately halophilic granular sludge to treat hypersaline wastewater.
Keyword :
Saltern sediments Saltern sediments Hypersaline wastewater Hypersaline wastewater Moderately halophilic granular sludge Moderately halophilic granular sludge Sulfated polysaccharides Sulfated polysaccharides Halomonas sp Halomonas sp
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GB/T 7714 | Wang, Ling , Cui, You -Wei , Jian, Li et al. Spontaneous granulation of moderately halophilic sludge inoculated with saltern sediments from single granule into multi-granule aggregation [J]. | ENVIRONMENTAL RESEARCH , 2023 , 216 . |
MLA | Wang, Ling et al. "Spontaneous granulation of moderately halophilic sludge inoculated with saltern sediments from single granule into multi-granule aggregation" . | ENVIRONMENTAL RESEARCH 216 (2023) . |
APA | Wang, Ling , Cui, You -Wei , Jian, Li , Yao, Jia-Lin . Spontaneous granulation of moderately halophilic sludge inoculated with saltern sediments from single granule into multi-granule aggregation . | ENVIRONMENTAL RESEARCH , 2023 , 216 . |
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Abstract :
Nitrogen and phosphate removal from wastewater relies on different functional bacteria. In this study, a novel strain affiliated with Pseudomonas aeruginosa was isolated from activated sludge by gradient dilution and per-formed heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal (HNADPR). The strain showed an ammonium removal efficiency of 87% and a phosphate removal efficiency of 97% under optimal conditions, such as C/N ratio of 10, P/N ratio of 0.1, temperature of 30C, and pH of 7.5-8.5. The modified Gompertz model could fit well the heterotrophic ammonium nitrification, aerobic nitrite/nitrate denitrification, and aerobic phosphate removal processes. Functional gene amplification indicated that ammonium removal followed the complete HN-AD pathway (NH4+ -> NH2OH -> NO2- -> NO3? -> NO2- -> NO -> N2O -> N-2). Phosphate removal only occurred under aerobic conditions and ceased under anaerobic conditions. In successive aerobic cycles, the strain persistently took up phosphate. In wastewater, phosphate was aerobically converted into cell membrane, intracellular and extracellular polymeric substrates (EPS). Phosphorus in the form of phosphate monoester was pooled in EPS. A hypothetic aerobic phosphate removal model for strain SNDPR-01 is proposed to improve our understanding of the novel bacterial function of HNADPR.
Keyword :
Aerobic phosphate removal model Aerobic phosphate removal model Phosphorus species Phosphorus species Heterotrophic nitrification-aerobic denitrification Heterotrophic nitrification-aerobic denitrification Simultaneous nitrogen and phosphate removal Simultaneous nitrogen and phosphate removal Nitrogen removal pathway Nitrogen removal pathway
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GB/T 7714 | Huang, Mei-Qi , Cui, You -Wei , Huang, Ji-Lin et al. A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal [J]. | WATER RESEARCH , 2022 , 221 . |
MLA | Huang, Mei-Qi et al. "A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal" . | WATER RESEARCH 221 (2022) . |
APA | Huang, Mei-Qi , Cui, You -Wei , Huang, Ji-Lin , Sun, Feng-Long , Chen, Si . A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal . | WATER RESEARCH , 2022 , 221 . |
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Abstract :
Sulfur-driven autotrophic denitrification (SADN) is a promising technology for nitrogen removal from wastewater. In this study, different-strength SMFs (0, 5, 20, 50, 70 mT) were evaluated to investigate the potential of external static magnetic field (SMF) for enriching sulfur-oxidizing autotrophic denitrifiers (SOAD). 50-mT and 70-mT SMFs were most suitable to accelerate the growth of SOAD and the elimination of non-SOAD. The relative abundance of Thiobacillus significantly increased (p < 0.01) from 6.26% in control reactor to 36.15% under 50 mT and 52.51% under 70 mT. Under 50 mT, Thiobacillus denitrificans accumulated most rapidly, with the largest population. Furthermore, functional gene forecast by high-throughput and metagenomic sequencing indicated that SMF changed the two-component system, the adenosine 5'-monophosphate-activated protein kinase (AMPK) signaling system, the phosphotransferase system (PTS), as well as N/S-related enzymes to regulate stress response and promote the growth of SOAD. The findings indicated that SMF accelerated the start-up of SADN.
Keyword :
Magnetic biological effect Magnetic biological effect Magnetic induction mechanism Magnetic induction mechanism Magnetic field Magnetic field Enrichment of functional bacteria Enrichment of functional bacteria Sulfur-driven autotrophic denitrification Sulfur-driven autotrophic denitrification
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GB/T 7714 | Yan, Hui-Juan , Cui, You-Wei , Han, Shi-Cai . Promoting enrichment of sulfur-oxidizing autotrophic denitrifiers via static magnetic fields: Performance and mechanism of magnetic biological effects [J]. | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
MLA | Yan, Hui-Juan et al. "Promoting enrichment of sulfur-oxidizing autotrophic denitrifiers via static magnetic fields: Performance and mechanism of magnetic biological effects" . | BIORESOURCE TECHNOLOGY 347 (2022) . |
APA | Yan, Hui-Juan , Cui, You-Wei , Han, Shi-Cai . Promoting enrichment of sulfur-oxidizing autotrophic denitrifiers via static magnetic fields: Performance and mechanism of magnetic biological effects . | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
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Abstract :
Low polyhydroxyalkanoate (PHA) volumetric productivity from wastewater limits low-cost PHA production. To resolve this problem, an external magnetic field (MF) coupled with upshock salinity was applied to PHA production by Haloferax mediterranei (family Halobacteriaceae). Elevating the fermentation salinity over the optimal growth salinity (200 g/L) increased the PHA cell content while inhibiting cell proliferation, decreasing volumetric productivity. When a MF of 50 mT in 300 g/L salinity was applied, H. mediterranei proliferation and PHA cell content were promoted, leading to a 7.95% increase in PHA volumetric productivity in synthetic molasses wastewater and a 13.82% increase in glucose feeding compared with those in 200 g/L salinity. Under the MF, osmotic pressure regulation was activated by accumulating K1 and increasing betaine synthesis. The maximum betaine content increased by 74.33% in 300 g/L salinity with a 50-mT MF compared with that in 200 g/L salinity. When a 50-mT MF in 300 g/L salinity was applied, the malondialdehyde (MDA) content decreased by 32.66% and the activity of superoxide dismutase (SOD) increased by 46.89%, which reduced the oxidative damage. This study provides a new solution to enhance PHA volumetric productivity by MF and an insight into the magnetic effects of H. mediterranei. IMPORTANCE The obstacle to replacing petroplastics with PHA is its high production cost. To increase the fermentation economy, a novel strategy of coupling a MF with salinity upshock was applied, which enhanced the PHA volumetric productivity of H. mediterranei in fermenting molasses wastewater. The magnetic effect of H. mediterranei was found at a MF of 50 mT, which improved the salt tolerance of H. mediterranei and reduced the oxidative damage induced by the elevated salinity, thereby promoting proliferation and PHA cell content. This is the first time a technical method for enhancing PHA volumetric productivity by means of a MF has been proposed. Such a strategy can advance the utilization of H. mediterranei for the industrial production of PHA using organic wastewater. Copyright © 2022 American Society for Microbiology. All Rights Reserved.
Keyword :
Haloferax mediterranei; magnetic field; microbial magnetic effect; polyhydroxyalkanoate volumetric productivity; salinity upshock Haloferax mediterranei; magnetic field; microbial magnetic effect; polyhydroxyalkanoate volumetric productivity; salinity upshock
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GB/T 7714 | Chen, S. , Cui, Y.-W. , Huang, M.-Q. . Coupling Magnetic Field and Salinity Upshock to Improve Polyhydroxyalkanoate Productivity by Haloferax mediterranei Feeding on Molasses Wastewater [J]. | Applied and Environmental Microbiology , 2022 , 88 (13) . |
MLA | Chen, S. et al. "Coupling Magnetic Field and Salinity Upshock to Improve Polyhydroxyalkanoate Productivity by Haloferax mediterranei Feeding on Molasses Wastewater" . | Applied and Environmental Microbiology 88 . 13 (2022) . |
APA | Chen, S. , Cui, Y.-W. , Huang, M.-Q. . Coupling Magnetic Field and Salinity Upshock to Improve Polyhydroxyalkanoate Productivity by Haloferax mediterranei Feeding on Molasses Wastewater . | Applied and Environmental Microbiology , 2022 , 88 (13) . |
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Abstract :
BACKGROUND Contact stabilization (CS) can be used to capture organic matters from wastewater. However, CS is less efficient in capturing soluble organic matters (SOMs). Recovery of SOMs is required to minimize carbon emission and to maximize organic recovery from wastewater. RESULTS SOMs were recovered by applying a feast/famine (F/F) regime in a CS process. Sludge retention time (SRT) was found to regulate the recovery efficiency of SOMs. At a SRT of 4 d, 44.7% of the removed soluble chemical oxygen demand (SCOD) in wastewater was recovered and stored into intracellular polyhydroxyalkanoates (PHA), leading to 12.8% decrease of CO2 emission in comparison with the conventional activated sludge process. The storage of PHA in waste sludge harvested at a SRT of 4 d significantly increased the methane yield (187.3 +/- 5.9 mL (g VSS)(-1)). The dominant bacteria performing SOM recovery in CS belonged to the genera Tessaracoccus, norank_f__Caldilineaceacea, unclassified_f__Microbacteriaceae and Flavobacterium. CONCLUSIONS The study demonstrated that applying F/F regime in a CS process can recover SOMs via PHA storage. The regulation of SRT can enhance SCOD recovery. The study provides a solution to enhance SOM recovery from wastewater instead of being oxidized into CO2. (c) 2022 Society of Chemical Industry (SCI).
Keyword :
polyhydroxyalkanoate polyhydroxyalkanoate contact stabilization contact stabilization organic recovery organic recovery feast feast famine regime famine regime methane yield methane yield
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GB/T 7714 | Sun, Feng-Long , Cui, You-Wei , Jian, Li et al. Sludge retention time effects on recovery of soluble organic matters via polyhydroxyalkanoate storage in contact stabilization process under feast/famine regime [J]. | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY , 2022 , 97 (12) : 3534-3543 . |
MLA | Sun, Feng-Long et al. "Sludge retention time effects on recovery of soluble organic matters via polyhydroxyalkanoate storage in contact stabilization process under feast/famine regime" . | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 97 . 12 (2022) : 3534-3543 . |
APA | Sun, Feng-Long , Cui, You-Wei , Jian, Li , Yao, Jia-Lin . Sludge retention time effects on recovery of soluble organic matters via polyhydroxyalkanoate storage in contact stabilization process under feast/famine regime . | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY , 2022 , 97 (12) , 3534-3543 . |
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