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学者姓名:崔有为

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Spontaneous granulation of moderately halophilic sludge inoculated with saltern sediments from single granule into multi-granule aggregation SCIE
期刊论文 | 2023 , 216 | ENVIRONMENTAL RESEARCH
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Abstract :

There is very limited research on the application of moderate halophiles for biotreatment of hypersaline wastewater widely generated from some industries. This study demonstrated the development of moderate halophiles inoculated from saltern sediments into aerobic granule sludge (AGS) to treat hypersaline wastewater with a salinity of 100 g/L. The granulation of moderate halophiles can occur without applying the settling ve-locity selective pressure. The saltern sediment initially aggregated into single small granules and finally devel-oped into 1200 +/- 50 mu m multiparticle granules. The halophiles affiliated in Halomonas was dominant in the granular bacterial community, with a relative abundance of 94.52%. Halomonas ventosae secreted sulfated polysaccharides. The sulfated polysaccharides content accounted for 63.95 +/- 2.10% in the polysaccharides (PS), having an adhesive role in connecting single granules. Multiparticle granules showed the clear stratified struc-ture, with alpha-D-glucopyranose polysaccharides in the inner bounders and beta-D-glucopyranose polysaccharides in the outer. The moderately granular sludge showed the stable chemical oxygen demand (COD) removal efficiency of >90% and the aerobic total inorganic nitrogen (TIN) removal efficiency (equal to ammonia removal) of 70 +/- 5.00%. This paper contributes new insight into the formation of moderately halophilic granular sludge and accelerates the application of moderately halophilic granular sludge to treat hypersaline wastewater.

Keyword :

Saltern sediments Saltern sediments Hypersaline wastewater Hypersaline wastewater Moderately halophilic granular sludge Moderately halophilic granular sludge Sulfated polysaccharides Sulfated polysaccharides Halomonas sp Halomonas sp

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GB/T 7714 Wang, Ling , Cui, You -Wei , Jian, Li et al. Spontaneous granulation of moderately halophilic sludge inoculated with saltern sediments from single granule into multi-granule aggregation [J]. | ENVIRONMENTAL RESEARCH , 2023 , 216 .
MLA Wang, Ling et al. "Spontaneous granulation of moderately halophilic sludge inoculated with saltern sediments from single granule into multi-granule aggregation" . | ENVIRONMENTAL RESEARCH 216 (2023) .
APA Wang, Ling , Cui, You -Wei , Jian, Li , Yao, Jia-Lin . Spontaneous granulation of moderately halophilic sludge inoculated with saltern sediments from single granule into multi-granule aggregation . | ENVIRONMENTAL RESEARCH , 2023 , 216 .
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Sludge retention time effects on recovery of soluble organic matters via polyhydroxyalkanoate storage in contact stabilization process under feast/famine regime SCIE
期刊论文 | 2022 , 97 (12) , 3534-3543 | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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BACKGROUND Contact stabilization (CS) can be used to capture organic matters from wastewater. However, CS is less efficient in capturing soluble organic matters (SOMs). Recovery of SOMs is required to minimize carbon emission and to maximize organic recovery from wastewater. RESULTS SOMs were recovered by applying a feast/famine (F/F) regime in a CS process. Sludge retention time (SRT) was found to regulate the recovery efficiency of SOMs. At a SRT of 4 d, 44.7% of the removed soluble chemical oxygen demand (SCOD) in wastewater was recovered and stored into intracellular polyhydroxyalkanoates (PHA), leading to 12.8% decrease of CO2 emission in comparison with the conventional activated sludge process. The storage of PHA in waste sludge harvested at a SRT of 4 d significantly increased the methane yield (187.3 +/- 5.9 mL (g VSS)(-1)). The dominant bacteria performing SOM recovery in CS belonged to the genera Tessaracoccus, norank_f__Caldilineaceacea, unclassified_f__Microbacteriaceae and Flavobacterium. CONCLUSIONS The study demonstrated that applying F/F regime in a CS process can recover SOMs via PHA storage. The regulation of SRT can enhance SCOD recovery. The study provides a solution to enhance SOM recovery from wastewater instead of being oxidized into CO2. (c) 2022 Society of Chemical Industry (SCI).

Keyword :

polyhydroxyalkanoate polyhydroxyalkanoate contact stabilization contact stabilization organic recovery organic recovery feast feast famine regime famine regime methane yield methane yield

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GB/T 7714 Sun, Feng-Long , Cui, You-Wei , Jian, Li et al. Sludge retention time effects on recovery of soluble organic matters via polyhydroxyalkanoate storage in contact stabilization process under feast/famine regime [J]. | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY , 2022 , 97 (12) : 3534-3543 .
MLA Sun, Feng-Long et al. "Sludge retention time effects on recovery of soluble organic matters via polyhydroxyalkanoate storage in contact stabilization process under feast/famine regime" . | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY 97 . 12 (2022) : 3534-3543 .
APA Sun, Feng-Long , Cui, You-Wei , Jian, Li , Yao, Jia-Lin . Sludge retention time effects on recovery of soluble organic matters via polyhydroxyalkanoate storage in contact stabilization process under feast/famine regime . | JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY , 2022 , 97 (12) , 3534-3543 .
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Promoting enrichment of sulfur-oxidizing autotrophic denitrifiers via static magnetic fields: Performance and mechanism of magnetic biological effects SCIE
期刊论文 | 2022 , 347 | BIORESOURCE TECHNOLOGY
WoS CC Cited Count: 7
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Abstract :

Sulfur-driven autotrophic denitrification (SADN) is a promising technology for nitrogen removal from wastewater. In this study, different-strength SMFs (0, 5, 20, 50, 70 mT) were evaluated to investigate the potential of external static magnetic field (SMF) for enriching sulfur-oxidizing autotrophic denitrifiers (SOAD). 50-mT and 70-mT SMFs were most suitable to accelerate the growth of SOAD and the elimination of non-SOAD. The relative abundance of Thiobacillus significantly increased (p < 0.01) from 6.26% in control reactor to 36.15% under 50 mT and 52.51% under 70 mT. Under 50 mT, Thiobacillus denitrificans accumulated most rapidly, with the largest population. Furthermore, functional gene forecast by high-throughput and metagenomic sequencing indicated that SMF changed the two-component system, the adenosine 5'-monophosphate-activated protein kinase (AMPK) signaling system, the phosphotransferase system (PTS), as well as N/S-related enzymes to regulate stress response and promote the growth of SOAD. The findings indicated that SMF accelerated the start-up of SADN.

Keyword :

Magnetic biological effect Magnetic biological effect Magnetic induction mechanism Magnetic induction mechanism Magnetic field Magnetic field Enrichment of functional bacteria Enrichment of functional bacteria Sulfur-driven autotrophic denitrification Sulfur-driven autotrophic denitrification

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GB/T 7714 Yan, Hui-Juan , Cui, You-Wei , Han, Shi-Cai . Promoting enrichment of sulfur-oxidizing autotrophic denitrifiers via static magnetic fields: Performance and mechanism of magnetic biological effects [J]. | BIORESOURCE TECHNOLOGY , 2022 , 347 .
MLA Yan, Hui-Juan et al. "Promoting enrichment of sulfur-oxidizing autotrophic denitrifiers via static magnetic fields: Performance and mechanism of magnetic biological effects" . | BIORESOURCE TECHNOLOGY 347 (2022) .
APA Yan, Hui-Juan , Cui, You-Wei , Han, Shi-Cai . Promoting enrichment of sulfur-oxidizing autotrophic denitrifiers via static magnetic fields: Performance and mechanism of magnetic biological effects . | BIORESOURCE TECHNOLOGY , 2022 , 347 .
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Occurrence of heterotrophic nitrification-aerobic denitrification induced by decreasing salinity in a halophilic AGS SBR treating hypersaline wastewater SCIE
期刊论文 | 2022 , 431 | CHEMICAL ENGINEERING JOURNAL
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Heterotrophic nitrification-aerobic denitrification (HN-AD) is a novel alternative for nitrogen removal due to simultaneous nitrification and denitrification that occurs under aerobic conditions. Until now, ways in which to achieve aerobic nitrogen removal from wastewater via the HN-AD process have not been fully explored. In this study, autotrophic nitrification was converted into HN-AD in a halophilic aerobic granular sludge (HAGS) system when the wastewater salinity decreased from 30 to 20 g/L. Bacteria affiliated to the genus Paracoccus were dominant in the HAGS with a relative abundance of 56%, functioning with HN-AD. Accordingly, the indicative gene napA encoding for HN-AD was 5.1 x 10(9) copies/g HAGS. HAGS performing HN-AD exhibited a stable total inorganic nitrogen (TIN) removal efficiency performance in response to salinity variations of 10-40 g/L, owing to the stable HN-AD functional community. The significant inhibition of HN-AD was observed in both low salinity (& LE;5 g/L) and high salinity (& GE;50 g/L) conditions, accompanied by the disintegration of granules. Granular disintegration was triggered by the lower secretion of extracellular polymeric substances and alginate-like exopolysaccharides. HAGS performing HN-AD could simultaneously remove chemical oxygen demand and TIN from saline wastewater in a single aerobic tank, thus reducing the construction and operation costs.

Keyword :

Halophilic AGS Halophilic AGS Paracoccus Paracoccus Salinity shock Salinity shock Heterotrophic nitrification-aerobic denitrification Heterotrophic nitrification-aerobic denitrification Species succession Species succession

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GB/T 7714 Huang, Ji-Lin , Cui, You-Wei , Yan, Jin-Lei et al. Occurrence of heterotrophic nitrification-aerobic denitrification induced by decreasing salinity in a halophilic AGS SBR treating hypersaline wastewater [J]. | CHEMICAL ENGINEERING JOURNAL , 2022 , 431 .
MLA Huang, Ji-Lin et al. "Occurrence of heterotrophic nitrification-aerobic denitrification induced by decreasing salinity in a halophilic AGS SBR treating hypersaline wastewater" . | CHEMICAL ENGINEERING JOURNAL 431 (2022) .
APA Huang, Ji-Lin , Cui, You-Wei , Yan, Jin-Lei , Cui, Yuan . Occurrence of heterotrophic nitrification-aerobic denitrification induced by decreasing salinity in a halophilic AGS SBR treating hypersaline wastewater . | CHEMICAL ENGINEERING JOURNAL , 2022 , 431 .
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A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal SCIE
期刊论文 | 2022 , 221 | WATER RESEARCH
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Nitrogen and phosphate removal from wastewater relies on different functional bacteria. In this study, a novel strain affiliated with Pseudomonas aeruginosa was isolated from activated sludge by gradient dilution and per-formed heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal (HNADPR). The strain showed an ammonium removal efficiency of 87% and a phosphate removal efficiency of 97% under optimal conditions, such as C/N ratio of 10, P/N ratio of 0.1, temperature of 30C, and pH of 7.5-8.5. The modified Gompertz model could fit well the heterotrophic ammonium nitrification, aerobic nitrite/nitrate denitrification, and aerobic phosphate removal processes. Functional gene amplification indicated that ammonium removal followed the complete HN-AD pathway (NH4+ -> NH2OH -> NO2- -> NO3? -> NO2- -> NO -> N2O -> N-2). Phosphate removal only occurred under aerobic conditions and ceased under anaerobic conditions. In successive aerobic cycles, the strain persistently took up phosphate. In wastewater, phosphate was aerobically converted into cell membrane, intracellular and extracellular polymeric substrates (EPS). Phosphorus in the form of phosphate monoester was pooled in EPS. A hypothetic aerobic phosphate removal model for strain SNDPR-01 is proposed to improve our understanding of the novel bacterial function of HNADPR.

Keyword :

Aerobic phosphate removal model Aerobic phosphate removal model Phosphorus species Phosphorus species Heterotrophic nitrification-aerobic denitrification Heterotrophic nitrification-aerobic denitrification Simultaneous nitrogen and phosphate removal Simultaneous nitrogen and phosphate removal Nitrogen removal pathway Nitrogen removal pathway

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GB/T 7714 Huang, Mei-Qi , Cui, You -Wei , Huang, Ji-Lin et al. A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal [J]. | WATER RESEARCH , 2022 , 221 .
MLA Huang, Mei-Qi et al. "A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal" . | WATER RESEARCH 221 (2022) .
APA Huang, Mei-Qi , Cui, You -Wei , Huang, Ji-Lin , Sun, Feng-Long , Chen, Si . A novel Pseudomonas aeruginosa strain performs simultaneous heterotrophic nitrification-aerobic denitrification and aerobic phosphate removal . | WATER RESEARCH , 2022 , 221 .
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Coupling Magnetic Field and Salinity Upshock to Improve Polyhydroxyalkanoate Productivity by Haloferax mediterranei Feeding on Molasses Wastewater Scopus
期刊论文 | 2022 , 88 (13) | Applied and Environmental Microbiology
SCOPUS Cited Count: 2
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Abstract :

Low polyhydroxyalkanoate (PHA) volumetric productivity from wastewater limits low-cost PHA production. To resolve this problem, an external magnetic field (MF) coupled with upshock salinity was applied to PHA production by Haloferax mediterranei (family Halobacteriaceae). Elevating the fermentation salinity over the optimal growth salinity (200 g/L) increased the PHA cell content while inhibiting cell proliferation, decreasing volumetric productivity. When a MF of 50 mT in 300 g/L salinity was applied, H. mediterranei proliferation and PHA cell content were promoted, leading to a 7.95% increase in PHA volumetric productivity in synthetic molasses wastewater and a 13.82% increase in glucose feeding compared with those in 200 g/L salinity. Under the MF, osmotic pressure regulation was activated by accumulating K1 and increasing betaine synthesis. The maximum betaine content increased by 74.33% in 300 g/L salinity with a 50-mT MF compared with that in 200 g/L salinity. When a 50-mT MF in 300 g/L salinity was applied, the malondialdehyde (MDA) content decreased by 32.66% and the activity of superoxide dismutase (SOD) increased by 46.89%, which reduced the oxidative damage. This study provides a new solution to enhance PHA volumetric productivity by MF and an insight into the magnetic effects of H. mediterranei. IMPORTANCE The obstacle to replacing petroplastics with PHA is its high production cost. To increase the fermentation economy, a novel strategy of coupling a MF with salinity upshock was applied, which enhanced the PHA volumetric productivity of H. mediterranei in fermenting molasses wastewater. The magnetic effect of H. mediterranei was found at a MF of 50 mT, which improved the salt tolerance of H. mediterranei and reduced the oxidative damage induced by the elevated salinity, thereby promoting proliferation and PHA cell content. This is the first time a technical method for enhancing PHA volumetric productivity by means of a MF has been proposed. Such a strategy can advance the utilization of H. mediterranei for the industrial production of PHA using organic wastewater. Copyright © 2022 American Society for Microbiology. All Rights Reserved.

Keyword :

Haloferax mediterranei; magnetic field; microbial magnetic effect; polyhydroxyalkanoate volumetric productivity; salinity upshock Haloferax mediterranei; magnetic field; microbial magnetic effect; polyhydroxyalkanoate volumetric productivity; salinity upshock

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GB/T 7714 Chen, S. , Cui, Y.-W. , Huang, M.-Q. . Coupling Magnetic Field and Salinity Upshock to Improve Polyhydroxyalkanoate Productivity by Haloferax mediterranei Feeding on Molasses Wastewater [J]. | Applied and Environmental Microbiology , 2022 , 88 (13) .
MLA Chen, S. et al. "Coupling Magnetic Field and Salinity Upshock to Improve Polyhydroxyalkanoate Productivity by Haloferax mediterranei Feeding on Molasses Wastewater" . | Applied and Environmental Microbiology 88 . 13 (2022) .
APA Chen, S. , Cui, Y.-W. , Huang, M.-Q. . Coupling Magnetic Field and Salinity Upshock to Improve Polyhydroxyalkanoate Productivity by Haloferax mediterranei Feeding on Molasses Wastewater . | Applied and Environmental Microbiology , 2022 , 88 (13) .
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The metabolic pathways of polyhydroxyalkanoates and exopolysaccharides synthesized by Haloferax mediterranei in response to elevated salinity. PubMed
期刊论文 | 2021 , 232 , 104065 | Journal of proteomics
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How polymer synthesis is mobilized or activated as a biological response of Haloferax mediterranei against hypertonic conditions remains largely unexplored. This study investigated the protein expression of H. mediterranei in response to high salinity by using isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis. The microbes were harvested at end of fermentation at the NaCl salinity of 75 and 250 g L-1. Among the identified 2123 proteins, 170 proteins were differentially expressed. Gene ontology annotation revealed that the highest number of proteins was annotated in biological process category, which was responsible for metabolic process, cellular component and catalytic activity. Differentially expressed proteins were belonged to the class of response to stimulus as well as catalytic activity and binding. Under high salinity conditions, three pathways were established as key responses of PHA and EPS production to hypertonic pressure. Two overexpressed proteins, beta-ketoacyl-ACP reductase and 3-hydroxyacyl-CoA dehydrogenase, enhanced the synthesis of PHAs. The serine-pyruvate transaminase and serine-glyoxylate transaminase were upregulated, thereby increasing the conversion of glucose to PHA. Downregulated levels of sulfate-adenylyl transferase and adenylyl-sulfate kinase could cause diminished EPS synthesis. This study could contribute to better understanding of the proteomic mechanisms of the synthesized polymers in defending against salt stress. SIGNIFICANCE: Haloferax mediterranei, a family member of halophilic archaea, is well known for its fermentative production of poly-β-hydroxyalkanoates (PHAs). PHAs are natural polymers that exhibit great potential in a wide range of applications such as a good alternative to petroleum-based plastics and the biocompatible material. For decades, the functional role of PHAs synthesized by H. mediterranei is deemed to be carbon and energy reservations. The finding proved that differential production of PHA and EPS in H. mediterranei exposed to elevated salinity was caused by differential protein expression. This is the first report on how PHA and EPS synthesized by H. mediterranei is mobilized as the response of increased salinity, contributing to the understanding of halophilic archaea's response to hypertonic stress and the precise control of fermentation production. Despite its advantages as a PHA cell factory, H. mediterranei synthesized EPS simultaneously, thereby lowering the maximum yield of PHA production. Overall, salinity can be used as a vital microbial fermentation parameter to obtain the highest harvest of PHA, as well as the lowest EPS synthesis in industrial fermentation.

Keyword :

Polyhydroxyalkanoates Polyhydroxyalkanoates Haloferax mediterranei Haloferax mediterranei iTRAQ analysis iTRAQ analysis Metabolism Metabolism Hypertonic condition Hypertonic condition Exopolysaccharide Exopolysaccharide

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GB/T 7714 Pacholak Amanda , Gao Ze-Liang , Gong Xiao-Yu et al. The metabolic pathways of polyhydroxyalkanoates and exopolysaccharides synthesized by Haloferax mediterranei in response to elevated salinity. [J]. | Journal of proteomics , 2021 , 232 : 104065 .
MLA Pacholak Amanda et al. "The metabolic pathways of polyhydroxyalkanoates and exopolysaccharides synthesized by Haloferax mediterranei in response to elevated salinity." . | Journal of proteomics 232 (2021) : 104065 .
APA Pacholak Amanda , Gao Ze-Liang , Gong Xiao-Yu , Kaczorek Ewa , Cui You-Wei . The metabolic pathways of polyhydroxyalkanoates and exopolysaccharides synthesized by Haloferax mediterranei in response to elevated salinity. . | Journal of proteomics , 2021 , 232 , 104065 .
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Red mud reuse for phosphate adsorption via zirconium modification: performance, kinetics, and mechanism SCIE
期刊论文 | 2021 , 225 , 331-339 | DESALINATION AND WATER TREATMENT
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The reuse of zirconium-modified red mud (Zr-RM) as a phosphate adsorbent for wastewater treatment was assessed herein. The physicochemical properties and phosphate adsorption performance of Zr-RM were investigated, revealing a single-layer adsorption pattern according to the Langmuir isotherm model. The adsorption rate followed a pseudo-second-order model. The maximum phosphate adsorption capacity was 33.14 mg/g and was positively correlated with temperature and initial phosphate concentration, and negatively correlated with the solution pH. In a solution containing fluoride and sulfate ions, due to competitive adsorption, the adsorption capacity of phosphate decreased. The material and wastewater treatment costs of Zr-RM are 0.99 and 61.3 $/ kg of P, respectively. This study showed a potential method for using RM with high value for environmental protection.

Keyword :

Zirconium modification Zirconium modification Adsorption Adsorption Red mud Red mud Phosphate removal Phosphate removal Waste reuse Waste reuse

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GB/T 7714 Li, Yan-Long , Alam, Fakhri , Cui, You-Wei . Red mud reuse for phosphate adsorption via zirconium modification: performance, kinetics, and mechanism [J]. | DESALINATION AND WATER TREATMENT , 2021 , 225 : 331-339 .
MLA Li, Yan-Long et al. "Red mud reuse for phosphate adsorption via zirconium modification: performance, kinetics, and mechanism" . | DESALINATION AND WATER TREATMENT 225 (2021) : 331-339 .
APA Li, Yan-Long , Alam, Fakhri , Cui, You-Wei . Red mud reuse for phosphate adsorption via zirconium modification: performance, kinetics, and mechanism . | DESALINATION AND WATER TREATMENT , 2021 , 225 , 331-339 .
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Carbon Source Applied in Enrichment Stage of Mixed Microbial Cultures Limits the Substrate Adaptability for PHA Fermentation Using the Renewable Carbon SCIE
期刊论文 | 2021 , 193 (10) , 3253-3270 | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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Suitability of different substrates for enriched mixed microbial cultures (MMCs) is of importance to the polyhydroxyalkanoate (PHA) fermentation using renewable carbon. In this study, three enriched MMCs were evaluated for their fermentation features and kinetics with different carbon sources (sodium acetate, glucose, or starch). The results showed that the highly specific bacterial community composition was developed depending on the applied carbon source. Correspondence analysis suggested that the genus affiliated in Gammaproteobacteria_unclassified was related to 3-hydroxybutyrate (HB) synthesis in acetate-fed MMC (relative abundance of 38%) and glucose-fed MMC (relative abundance of 76.7%), whereas Vibrio genus was related to 3-hydroxyvalerate (HV) production in glucose-fed MMC (relative abundance of 0.4%) and starch-fed MMC (relative abundance of 94.6%). The acetate-fed MMC could not use glucose and starch as fermentation carbon sources, showing the limitation of microbial species developed with the specific metabolic substrate. Glucose-fed MMC produced the highest PHA cell content of 64.2% cell dry weight when using sodium acetate as the fermentation carbon. Glucose-fed MMC showed wide resilience and adaptation to various carbon sources. When actual landfill leachate was used for fermentation by glucose-fed MMC, maximum PHA cell content of 45.5% cell dry weight and the PHA volumetric productivity of 0.265 g PHA/(L center dot h) were obtained. This study suggested carbon sources applied in the MMC enrichment stage had a significant influence on utilization of carbon in the fermentation stage.

Keyword :

Mixed microbial culture (MMC) Mixed microbial culture (MMC) Fermentation Fermentation Landfill leachate Landfill leachate Polyhydroxyalkanoates (PHAs) Polyhydroxyalkanoates (PHAs) Carbon source adaptation Carbon source adaptation

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GB/T 7714 Zhao, Jin , Cui, You-Wei , Zhang, Hong-Yu et al. Carbon Source Applied in Enrichment Stage of Mixed Microbial Cultures Limits the Substrate Adaptability for PHA Fermentation Using the Renewable Carbon [J]. | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY , 2021 , 193 (10) : 3253-3270 .
MLA Zhao, Jin et al. "Carbon Source Applied in Enrichment Stage of Mixed Microbial Cultures Limits the Substrate Adaptability for PHA Fermentation Using the Renewable Carbon" . | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY 193 . 10 (2021) : 3253-3270 .
APA Zhao, Jin , Cui, You-Wei , Zhang, Hong-Yu , Gao, Ze-Liang . Carbon Source Applied in Enrichment Stage of Mixed Microbial Cultures Limits the Substrate Adaptability for PHA Fermentation Using the Renewable Carbon . | APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY , 2021 , 193 (10) , 3253-3270 .
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A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification. PubMed
期刊论文 | 2021 , 333 , 125189 | Bioresource technology
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The utilization of halophilic bioresources is limited due to a lack of isolation and characterization work. A halophilic bacterium strain SND-01 of Exiguobacterium mexicanum was isolated in this study, which is the first report on its novel function in heterotrophic nitrification-aerobic denitrification (HN-AD). The strain SND-01 is slightly halophilic, surviving at 0 up to 9% (w/v) salinity. When utilizing ammonium, nitrate or nitrite as the sole nitrogen source in aerobic conditions, the isolated strain showed the maximum nitrogen removal rate of 2.24 ± 0.14 mg/(L·h), 3.63 ± 0.21 mg/(L·h) and 2.30 ± 0.23 mg/(L·h), respectively. Functional genes and key enzymes involved in heterotrophic-aerobic nitrogen transformations were characterized, establishing the pathway of HN-AD. The nitrogen removal via HN-AD is dependent on the C/N ratio, salinity and temperature. The halophilic Exiguobacterium mexicanum strain SND-01 shows a significant potential in biotreatment of saline wastewater in an easy and cost-effective way.

Keyword :

Saline wastewater Saline wastewater Nitrogen removal Nitrogen removal Aerobic denitrification Aerobic denitrification Halophilic bacterium Halophilic bacterium Heterotrophic nitrification Heterotrophic nitrification

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GB/T 7714 Cui Yuan , Cui You-Wei , Huang Ji-Lin . A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification. [J]. | Bioresource technology , 2021 , 333 : 125189 .
MLA Cui Yuan et al. "A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification." . | Bioresource technology 333 (2021) : 125189 .
APA Cui Yuan , Cui You-Wei , Huang Ji-Lin . A novel halophilic Exiguobacterium mexicanum strain removes nitrogen from saline wastewater via heterotrophic nitrification and aerobic denitrification. . | Bioresource technology , 2021 , 333 , 125189 .
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