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学者姓名：周志祥
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Abstract ：
Background Gout is a common inflammatory arthritis, which is mainly caused by the deposition of monosodium urate (MSU) in tissues. Transcriptomics was used to explore the pathogenesis and treatment of gout in our work.Objective The objective of the study was to analyze and validate potential therapeutic targets and biomarkers in THP-1 cells that were exposed to MSU.Methods THP-1 cells were exposed to MSU. The inflammatory effect was characterized, and RNA-Seq analysis was then carried out. The differential genes obtained by RNA-Seq were analyzed with gene expression omnibus (GEO) series 160170 (GSE160170) gout-related clinical samples in the GEO database and gout-related genes in the GeneCards database. From the three analysis approaches, the genes with significant differences were verified by the differential genes' transcription levels. The interaction relationship of long non-coding RNA (lncRNA) was proposed by ceRNA network analysis.Results MSU significantly promoted the release of IL-1 beta and IL-18 in THP-1 cells, which aggravated their inflammatory effect. Through RNA-Seq, 698 differential genes were obtained, including 606 differential mRNA and 92 differential `LncRNA. Cross-analysis of the RNA-Seq differential genes, the GSE160170 differential genes, and the gout-related genes in GeneCards revealed a total of 17 genes coexisting in the tripartite data. Furthermore, seven differential genes-C-X-C motif chemokine ligand 8 (CXCL8), C-X-C motif chemokine ligand 2 (CXCL2), tumor necrosis factor (TNF), C-C motif chemokine ligand 3 (CCL3), suppressor of cytokine signaling 3 (SOCS3), oncostatin M (OSM), and MIR22 host gene (MIR22HG)-were verified as key genes that analyzed the weight of genes in pathways, the enrichment of inflammation-related pathways, and protein-protein interaction (PPI) nodes combined with the expression of genes in RNA-Seq and GSE160170. It is suggested that MIR22HG may regulate OSM and SOCS3 through microRNA 4271 (miR-4271), OSM, and SOCS3m; CCL3 through microRNA 149-3p (miR-149-3p); and CXCL2 through microRNA 4652-3p (miR-4652-3p).Conclusion The potential of CXCL8, CXCL2, TNF, CCL3, SOCS3, and OSM as gout biomarkers and MIR22HG as a therapeutic target for gout are proposed, which provide new insights into the mechanisms of gout biomarkers and therapeutic methods.
Keyword ：
Gouty arthritis Gouty arthritis monosodium urate monosodium urate protein-protein interaction protein-protein interaction transcriptomic analysis transcriptomic analysis biomarker biomarker competitive endogenous RNA competitive endogenous RNA
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| GB/T 7714 | Wang, Guozhen , Liu, Zijia , Zheng, Yuchen et al. Transcriptomic Analysis of THP-1 Cells Exposed by Monosodium Urate Reveals Key Genes Involved in Gout [J]. | COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING , 2024 , 27 (18) : 2741-2752 . |
| MLA | Wang, Guozhen et al. "Transcriptomic Analysis of THP-1 Cells Exposed by Monosodium Urate Reveals Key Genes Involved in Gout" . | COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING 27 . 18 (2024) : 2741-2752 . |
| APA | Wang, Guozhen , Liu, Zijia , Zheng, Yuchen , Sheng, Chao , Hou, Xiaonan , Yao, Mengfei et al. Transcriptomic Analysis of THP-1 Cells Exposed by Monosodium Urate Reveals Key Genes Involved in Gout . | COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING , 2024 , 27 (18) , 2741-2752 . |
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Abstract ：
本发明涉及一种跨上皮/内皮细胞电阻仪及其测量方法，其主控板的输入端与固定电阻和测试电极并联，主控板的电源端与外部供电电源连接；测试电极采用银线电极。采用细胞悬浮液进行铺板，待细胞贴壁后，将内室含血清的培养基采用无血清的DMEM溶液替换，外室内的溶液也采用无血清的DMEM溶液替换；由具有银线电极的跨上皮/内皮细胞电阻仪测量空白孔的电阻值，并将空白孔的内室和外室均换成DMEM溶液，此时将Transwell小室转移到测板中；铺板完成后由具有银线电极的跨上皮/内皮细胞电阻仪连续多天测量内皮细胞的TEER值；将每天测量多个内皮细胞的TEER值求均值，根据每天测量结果的均值得到同细胞生长曲线具有一致趋势的TEER值的结果图，作为衡量上皮/内皮细胞屏障形成的定量标准。
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| GB/T 7714 | 周志祥 , 侯潇楠 , 姚梦菲 et al. 一种跨上皮/内皮细胞电阻仪及其测量方法 : CN202310597170.1[P]. | 2023-05-25 . |
| MLA | 周志祥 et al. "一种跨上皮/内皮细胞电阻仪及其测量方法" : CN202310597170.1. | 2023-05-25 . |
| APA | 周志祥 , 侯潇楠 , 姚梦菲 , 唐铎 , 刘紫佳 , 郑雨晨 et al. 一种跨上皮/内皮细胞电阻仪及其测量方法 : CN202310597170.1. | 2023-05-25 . |
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Abstract ：
Human papillomavirus (HPV) infection is one of the main causes of esophageal carcinoma (ESCA), and its carcinogenic mechanisms in ESCA require further investigation. E6 and E7 are HPV oncogenes, and their genomic integration is a crucial reason for the transformation of host cells into cancer cells. In order to reveal the role of oncogenes E6 and E7 in ESCA cells, the RNA-Seq raw data for HPV18-positive and -negative esophageal squamous cell carcinoma (ESCC) samples derived from the NCBI BioProject database were analyzed, and the differentially expressed genes were identified. Moreover, differentially expressed genes were enriched significantly in multiple cell death pathways, including apoptosis (cyclin-dependent kinase inhibitor 2A, plakophilin 1 and desmoglein 3), pyroptosis (gasdermin A, gasdermin C, NLR family pyrin domain containing 3, absent in melanoma 2, NLR family pyrin domain containing 1 and Toll like receptor 1) and autophagy (Unc-51 like autophagy activating kinase 1, adrenoceptor beta 2). Consequently, the effects of cisplatin-induced apoptosis and Hank's balanced salt solution-induced autophagy, and alpha-ketoglutarate-induced pyroptosis in the ESCC-expressing E6 and E7 cells were verified. Therefore, the expression of E6E7 may culminate in the inhibition of multiple cell death modes, which may also be one of the mechanisms of oncogene-induced carcinogenesis.
Keyword ：
esophageal squamous cell carcinoma esophageal squamous cell carcinoma regulated cell death regulated cell death transcriptomic transcriptomic human papilloma virus 18 human papilloma virus 18
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| GB/T 7714 | Tang, Duo , Wang, Guozhen , Liu, Zijia et al. Transcriptomic analysis of the effects of the HPV18 E6E7 gene on the cell death mode in esophageal squamous cell carcinoma [J]. | ONCOLOGY LETTERS , 2023 , 25 (4) . |
| MLA | Tang, Duo et al. "Transcriptomic analysis of the effects of the HPV18 E6E7 gene on the cell death mode in esophageal squamous cell carcinoma" . | ONCOLOGY LETTERS 25 . 4 (2023) . |
| APA | Tang, Duo , Wang, Guozhen , Liu, Zijia , Wang, Biqi , Yao, Mengfei , Wang, Qian et al. Transcriptomic analysis of the effects of the HPV18 E6E7 gene on the cell death mode in esophageal squamous cell carcinoma . | ONCOLOGY LETTERS , 2023 , 25 (4) . |
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Abstract ：
Oncogene E6 plays a critical role in the development and progression of esophageal cancer caused by human papillomavirus (HPV) infection. Alpha-ketoglutarate (AKG) is a key metabolite in the tricarboxylic acid cycle and has been widely used as a dietary and anti-ageing supplement. In this study, we found that treating esophageal squamous carcinoma cells with a high dose of AKG can induce cell pyroptosis. Furthermore, our research confirms that HPV18 E6 inhibits AKG-induced pyroptosis of esophageal squamous carcinoma cells by lowering P53 expression. P53 downregulates malate dehydrogenase 1 (MDH1) expression; however, MDH1 downregulates L-2-hydroxyglutarate (L-2HG) expression, which inhibits a rise in reactive oxygen species (ROS) levels-as L-2HG is responsible for excessive ROS. This study reveals the actuating mechanism behind cell pyroptosis of esophageal squamous carcinoma cells induced by high concentrations of AKG, and we posit the molecular pathway via which the HPV E6 oncoprotein inhibits cell pyroptosis.
Keyword ：
pyroptosis pyroptosis ROS ROS & alpha;-ketoglutarate & alpha;-ketoglutarate GSDMC GSDMC E6 oncogene E6 oncogene HPV HPV
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| GB/T 7714 | Tang, Duo , Zheng, Yuchen , Wang, Guozhen et al. HPV18 E6 inhibits & alpha;-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway [J]. | FEBS OPEN BIO , 2023 , 13 (8) : 1522-1535 . |
| MLA | Tang, Duo et al. "HPV18 E6 inhibits & alpha;-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway" . | FEBS OPEN BIO 13 . 8 (2023) : 1522-1535 . |
| APA | Tang, Duo , Zheng, Yuchen , Wang, Guozhen , Sheng, Chao , Liu, Zijia , Wang, Biqi et al. HPV18 E6 inhibits & alpha;-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway . | FEBS OPEN BIO , 2023 , 13 (8) , 1522-1535 . |
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Abstract ：
Objective To establish a sensitive, simple and rapid detection method for African swine fever virus (ASFV) B646L gene. Methods A recombinase-aided amplification-lateral flow dipstick (RAA-LFD) assay was developed in this study. Recombinase-aided amplification (RAA) is used to amplify template DNA, and lateral flow dipstick (LFD) is used to interpret the results after the amplification is completed. The lower limits of detection and specificity of the RAA assay were verified using recombinant plasmid and pathogenic nucleic acid. In addition, 30 clinical samples were tested to evaluate the performance of the RAA assay. Results The RAA-LFD assay was completed within 15 min at 37 degrees C, including 10 min for nucleic acid amplification and 5 minutes for LFD reading results. The detection limit of this assay was found to be 200 copies per reaction. And there was no cross-reactivity with other swine viruses. Conclusion A highly sensitive, specific, and simple RAA-LFD method was developed for the rapid detection of the ASFV.
Keyword ：
Lateral flow detection Lateral flow detection African swine fever virus African swine fever virus Recombinase aided amplification Recombinase aided amplification
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| GB/T 7714 | Li Jiang Shuai , Hao Yan Zhe , Hou Mei Ling et al. Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus [J]. | BIOMEDICAL AND ENVIRONMENTAL SCIENCES , 2022 , 35 (2) : 133-140 . |
| MLA | Li Jiang Shuai et al. "Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus" . | BIOMEDICAL AND ENVIRONMENTAL SCIENCES 35 . 2 (2022) : 133-140 . |
| APA | Li Jiang Shuai , Hao Yan Zhe , Hou Mei Ling , Zhang Xuan , Zhang Xiao Guang , Cao Yu Xi et al. Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus . | BIOMEDICAL AND ENVIRONMENTAL SCIENCES , 2022 , 35 (2) , 133-140 . |
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Abstract ：
A random laser carrying the scattering information on a biological host is a promising tool for the characterization of biophysical properties. In this work, random lasing from label-free living cells is proposed to achieve rapid cytometry of apoptosis. Random lasing is achieved by adding biocompatible gain medium to a confocal dish containing cells under optically pumped conditions. The random lasing characteristics are distinct at different stages of cell apoptosis after drug treatment. By analyzing the power Fourier transform results of the random lasing spectra, the percentage of apoptotic cells could be distinguished within two seconds, which is more than an order of magnitude faster than traditional flow cytometry. These results provide a label-free approach for rapid cytometry of apoptosis, which is advantageous for further research of random lasers in the biological field.
Keyword ：
cytometry cytometry living cells living cells random lasing random lasing label-free label-free apoptosis apoptosis
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| GB/T 7714 | Xu, Zhiyang , Hong, Qihao , Ge, Kun et al. Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis [J]. | NANO LETTERS , 2022 , 22 (1) : 172-178 . |
| MLA | Xu, Zhiyang et al. "Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis" . | NANO LETTERS 22 . 1 (2022) : 172-178 . |
| APA | Xu, Zhiyang , Hong, Qihao , Ge, Kun , Shi, Xiaoyu , Wang, Xiaolei , Deng, Jinxiang et al. Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis . | NANO LETTERS , 2022 , 22 (1) , 172-178 . |
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Abstract ：
在课程中融入思政教育是实现"三全育人"的关键途径.拓宽创新性教育的视野,结合工科院校分子肿瘤学课程的特点和优势,深层次挖掘思政元素,通过修订课程教学目标、创新教学模式、改革考核评价机制,将思政教育引入课堂教学中,实现知识传授与价值引领的有机统一,为培养和引导研究生树立正确的人生观和价值观奠定了基础.
Keyword ：
教学改革 教学改革 课程思政 课程思政 分子肿瘤学 分子肿瘤学 案例教学 案例教学
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| GB/T 7714 | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 [J]. | 现代职业教育 , 2021 , (42) : 20-21 . |
| MLA | 张芳 et al. "新工科背景下研究生课程分子肿瘤学思政建设初探" . | 现代职业教育 42 (2021) : 20-21 . |
| APA | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 . | 现代职业教育 , 2021 , (42) , 20-21 . |
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Abstract ：
Exposure to airborne particulate matter (PM2.5) is associated with cardiovascular diseases. In order to investigate the molecular mechanisms of air pollution-induced CVDs toxicity, human umbilical vein endothelial cells (HUVECs) were exposed to PM2.5 collected from January, 2016 winter in Beijing, China. We performed RNA sequencing to elucidate key molecular mechanism of PM 2.5-mediated toxicity in HUVECs. A total of 1753 genes, 864 up-regulated and 889 down-regulated, were observed to be differentially expressed genes (DEGs). Among these, genes involved in metabolic response, oxidative stress, inflammatory response, and vascular dysfunction were significantly differentially expressed (log2 FC > 4). The results were validated by quantitative real-time PCR (qPCR) and Western blot for CYP1B1, HMOX1, IL8, and GJA4. Pathway analysis revealed that DEGs were involved in the biological processes related to metabolism, inflammation, and host defense against environmental insults. This research is providing a further understanding of the mechanisms underlying PM2.5-induced cardiovascular diseases (CVDs).
Keyword ：
5 5 CVDs CVDs RNA-seq RNA-seq PM2 PM2 HUVEC HUVEC
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| GB/T 7714 | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis [J]. | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 , 32 (9) : 2052-2064 . |
| MLA | Zhou, Zhixiang et al. "Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis" . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH 32 . 9 (2021) : 2052-2064 . |
| APA | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara , Li, Wenke , Niu, Bingyu , Li, Jiangshuai et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 , 32 (9) , 2052-2064 . |
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Abstract ：
Antibiotic resistance genes (ARGs) were considered as emerging genetic contaminants, which were threatening public health worldwide. ARGs had two forms of existence: intracellular ARGs (i-ARGs) and extracellular ARGs (e-ARGs), and their emergence and spread patterns were rarely revealed in aerobic granular sludge (AGS) system. The results showed that both i-ARGs and e-ARGs were enriched but had different shift patterns during AGS cultivation process, and e-ARGs had higher growth rate. Most functional bacteria enriched in AGS might play important roles in accumulating of i/e-ARGs, and their oligotypes might be the key reason for the different correlations between taxa and i/e-ARGs. E-ARGs might be transformed from i-ARGs and mainly carried by eintegrons, and three major modules of co-occurrence among e-ARGs were observed. The combined action of environmental factors and bacterial community contributed most (35.43%) to the shift pattern of e-ARGs. This study deciphered the migrations and accumulations of i/e-ARGs during aerobic granulation process, and provided several meaningful comprehensions on i/e-ARGs dissemination during practical AGS application.
Keyword ：
I I Aerobic granulation process Aerobic granulation process Oligotypes Oligotypes Functional bacteria Functional bacteria e-antibiotic resistance genes e-antibiotic resistance genes Co-occurrence modules Co-occurrence modules
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| GB/T 7714 | Li, Dingchang , Gao, Jingfeng , Dai, Huihui et al. Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process [J]. | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
| MLA | Li, Dingchang et al. "Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process" . | CHEMICAL ENGINEERING JOURNAL 421 (2021) . |
| APA | Li, Dingchang , Gao, Jingfeng , Dai, Huihui , Duan, Wanjun , Wang, Zhiqi , Zhou, Zhixiang . Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process . | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
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Abstract ：
为研究肺水通道蛋白1(AQP1)在PM2.5暴露引起肺损伤中的作用,本文首先在细胞水平上检测了PM2.5暴露对肺上皮细胞A549的细胞存活率、细胞AQP1的mRNA和蛋白表达变化,以及AQP1在细胞内的表达定位的影响.另外,将10只雄性SD大鼠随机分为2组:对照组和PM2.5染毒组(1.5 mg·kg-1体重),每组5只,采用气管滴注法染毒,每隔1d染毒1次,共2周,制备PM2.5暴露大鼠模型,进一步观察PM2.5暴露对大鼠肺AQP1表达及肺组织损伤的影响情况.结果显示,PM2.5暴露可引起肺上皮细胞A549形态异常和存活率降低.PM2.5暴露后的24 h内A549细胞的AQP1 mRNA及蛋白相对表达水平先升高,并在暴露后的12h内达到最高水平,然后有所下降;免疫荧光结果也证实AQP1蛋白在PM25暴露后表达水平升高,并呈现从细胞质向细胞膜定位的过程;用PM2.5暴露处理SD大鼠肺组织中AQP1表达亦显著增加,并且肺组织出现肺泡隔增厚,并有广泛的水肿充血及炎症细胞浸润.总之,PM2.5暴露可诱导A549细胞及大鼠肺组织内AQP1的表达升高,可能参与PM2.5暴露引起的肺病理性损伤过程.
Keyword ：
肺上皮细胞 肺上皮细胞 PM25 PM25 急性肺损伤 急性肺损伤 AQP1 AQP1
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| GB/T 7714 | 李文可 , 牛冰羽 , 李疆帅 et al. PM2.5暴露对肺AQP1表达的影响研究 [J]. | 环境科学学报 , 2020 , 40 (6) : 2255-2261 . |
| MLA | 李文可 et al. "PM2.5暴露对肺AQP1表达的影响研究" . | 环境科学学报 40 . 6 (2020) : 2255-2261 . |
| APA | 李文可 , 牛冰羽 , 李疆帅 , 洪启浩 , 苗晓燕 , 周志祥 . PM2.5暴露对肺AQP1表达的影响研究 . | 环境科学学报 , 2020 , 40 (6) , 2255-2261 . |
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