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学者姓名:周志祥
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Abstract :
Oncogene E6 plays a critical role in the development and progression of esophageal cancer caused by human papillomavirus (HPV) infection. Alpha-ketoglutarate (AKG) is a key metabolite in the tricarboxylic acid cycle and has been widely used as a dietary and anti-ageing supplement. In this study, we found that treating esophageal squamous carcinoma cells with a high dose of AKG can induce cell pyroptosis. Furthermore, our research confirms that HPV18 E6 inhibits AKG-induced pyroptosis of esophageal squamous carcinoma cells by lowering P53 expression. P53 downregulates malate dehydrogenase 1 (MDH1) expression; however, MDH1 downregulates L-2-hydroxyglutarate (L-2HG) expression, which inhibits a rise in reactive oxygen species (ROS) levels-as L-2HG is responsible for excessive ROS. This study reveals the actuating mechanism behind cell pyroptosis of esophageal squamous carcinoma cells induced by high concentrations of AKG, and we posit the molecular pathway via which the HPV E6 oncoprotein inhibits cell pyroptosis.
Keyword :
pyroptosis pyroptosis ROS ROS & alpha;-ketoglutarate & alpha;-ketoglutarate GSDMC GSDMC E6 oncogene E6 oncogene HPV HPV
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GB/T 7714 | Tang, Duo , Zheng, Yuchen , Wang, Guozhen et al. HPV18 E6 inhibits & alpha;-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway [J]. | FEBS OPEN BIO , 2023 , 13 (8) : 1522-1535 . |
MLA | Tang, Duo et al. "HPV18 E6 inhibits & alpha;-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway" . | FEBS OPEN BIO 13 . 8 (2023) : 1522-1535 . |
APA | Tang, Duo , Zheng, Yuchen , Wang, Guozhen , Sheng, Chao , Liu, Zijia , Wang, Biqi et al. HPV18 E6 inhibits & alpha;-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway . | FEBS OPEN BIO , 2023 , 13 (8) , 1522-1535 . |
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Abstract :
Human papillomavirus (HPV) infection is one of the main causes of esophageal carcinoma (ESCA), and its carcinogenic mechanisms in ESCA require further investigation. E6 and E7 are HPV oncogenes, and their genomic integration is a crucial reason for the transformation of host cells into cancer cells. In order to reveal the role of oncogenes E6 and E7 in ESCA cells, the RNA-Seq raw data for HPV18-positive and -negative esophageal squamous cell carcinoma (ESCC) samples derived from the NCBI BioProject database were analyzed, and the differentially expressed genes were identified. Moreover, differentially expressed genes were enriched significantly in multiple cell death pathways, including apoptosis (cyclin-dependent kinase inhibitor 2A, plakophilin 1 and desmoglein 3), pyroptosis (gasdermin A, gasdermin C, NLR family pyrin domain containing 3, absent in melanoma 2, NLR family pyrin domain containing 1 and Toll like receptor 1) and autophagy (Unc-51 like autophagy activating kinase 1, adrenoceptor beta 2). Consequently, the effects of cisplatin-induced apoptosis and Hank's balanced salt solution-induced autophagy, and alpha-ketoglutarate-induced pyroptosis in the ESCC-expressing E6 and E7 cells were verified. Therefore, the expression of E6E7 may culminate in the inhibition of multiple cell death modes, which may also be one of the mechanisms of oncogene-induced carcinogenesis.
Keyword :
esophageal squamous cell carcinoma esophageal squamous cell carcinoma regulated cell death regulated cell death transcriptomic transcriptomic human papilloma virus 18 human papilloma virus 18
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GB/T 7714 | Tang, Duo , Wang, Guozhen , Liu, Zijia et al. Transcriptomic analysis of the effects of the HPV18 E6E7 gene on the cell death mode in esophageal squamous cell carcinoma [J]. | ONCOLOGY LETTERS , 2023 , 25 (4) . |
MLA | Tang, Duo et al. "Transcriptomic analysis of the effects of the HPV18 E6E7 gene on the cell death mode in esophageal squamous cell carcinoma" . | ONCOLOGY LETTERS 25 . 4 (2023) . |
APA | Tang, Duo , Wang, Guozhen , Liu, Zijia , Wang, Biqi , Yao, Mengfei , Wang, Qian et al. Transcriptomic analysis of the effects of the HPV18 E6E7 gene on the cell death mode in esophageal squamous cell carcinoma . | ONCOLOGY LETTERS , 2023 , 25 (4) . |
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Abstract :
Objective To establish a sensitive, simple and rapid detection method for African swine fever virus (ASFV) B646L gene. Methods A recombinase-aided amplification-lateral flow dipstick (RAA-LFD) assay was developed in this study. Recombinase-aided amplification (RAA) is used to amplify template DNA, and lateral flow dipstick (LFD) is used to interpret the results after the amplification is completed. The lower limits of detection and specificity of the RAA assay were verified using recombinant plasmid and pathogenic nucleic acid. In addition, 30 clinical samples were tested to evaluate the performance of the RAA assay. Results The RAA-LFD assay was completed within 15 min at 37 degrees C, including 10 min for nucleic acid amplification and 5 minutes for LFD reading results. The detection limit of this assay was found to be 200 copies per reaction. And there was no cross-reactivity with other swine viruses. Conclusion A highly sensitive, specific, and simple RAA-LFD method was developed for the rapid detection of the ASFV.
Keyword :
Lateral flow detection Lateral flow detection African swine fever virus African swine fever virus Recombinase aided amplification Recombinase aided amplification
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GB/T 7714 | Li Jiang Shuai , Hao Yan Zhe , Hou Mei Ling et al. Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus [J]. | BIOMEDICAL AND ENVIRONMENTAL SCIENCES , 2022 , 35 (2) : 133-140 . |
MLA | Li Jiang Shuai et al. "Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus" . | BIOMEDICAL AND ENVIRONMENTAL SCIENCES 35 . 2 (2022) : 133-140 . |
APA | Li Jiang Shuai , Hao Yan Zhe , Hou Mei Ling , Zhang Xuan , Zhang Xiao Guang , Cao Yu Xi et al. Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus . | BIOMEDICAL AND ENVIRONMENTAL SCIENCES , 2022 , 35 (2) , 133-140 . |
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Abstract :
A random laser carrying the scattering information on a biological host is a promising tool for the characterization of biophysical properties. In this work, random lasing from label-free living cells is proposed to achieve rapid cytometry of apoptosis. Random lasing is achieved by adding biocompatible gain medium to a confocal dish containing cells under optically pumped conditions. The random lasing characteristics are distinct at different stages of cell apoptosis after drug treatment. By analyzing the power Fourier transform results of the random lasing spectra, the percentage of apoptotic cells could be distinguished within two seconds, which is more than an order of magnitude faster than traditional flow cytometry. These results provide a label-free approach for rapid cytometry of apoptosis, which is advantageous for further research of random lasers in the biological field.
Keyword :
cytometry cytometry living cells living cells random lasing random lasing label-free label-free apoptosis apoptosis
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GB/T 7714 | Xu, Zhiyang , Hong, Qihao , Ge, Kun et al. Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis [J]. | NANO LETTERS , 2022 , 22 (1) : 172-178 . |
MLA | Xu, Zhiyang et al. "Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis" . | NANO LETTERS 22 . 1 (2022) : 172-178 . |
APA | Xu, Zhiyang , Hong, Qihao , Ge, Kun , Shi, Xiaoyu , Wang, Xiaolei , Deng, Jinxiang et al. Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis . | NANO LETTERS , 2022 , 22 (1) , 172-178 . |
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Abstract :
Oesophageal cancer is one of the deadliest cancers in the world. Oesophageal squamous cell carcinoma (ESCC) is the most prevalent histological type of oesophageal cancer. Oesophageal cancer has a poor prognosis because of its invasiveness. Thus, it is especially important to seek effective treatment methods. Research indicates that long non-coding RNAs (lncRNAs) play a significant role in the occurrence and development of oesophageal cancer. The aim of this study was to describe the role of LINC00958 in ESCC. Bioinformatics and real-time quantitative polymerase chain reaction (RT-qPCR) methods were utilized to predict and verify the expression of LINC00958 in ESCC. Related functional experiments, including cell proliferation, migration and invasion, were performed. In addition, a western blot and a dual luciferase reporter gene experiment were used to study the detailed carcinogenic mechanism of LINC00958. The results indicated there was a high expression of LINC00958 in ESCC, which promoted proliferation, migration, invasion and Epithelial-Mesenchymal Transition (EMT) of ESCC cells, and this effect may be via regulating miR-510-5p.
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GB/T 7714 | Wang Biqi , Tang Duo , Liu Zijia et al. LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells. [J]. | PloS one , 2021 , 16 (5) : e0251797 . |
MLA | Wang Biqi et al. "LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells." . | PloS one 16 . 5 (2021) : e0251797 . |
APA | Wang Biqi , Tang Duo , Liu Zijia , Wang Qian , Xue Shan , Zhao Zijie et al. LINC00958 promotes proliferation, migration, invasion, and epithelial-mesenchymal transition of oesophageal squamous cell carcinoma cells. . | PloS one , 2021 , 16 (5) , e0251797 . |
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Abstract :
Exposure to airborne particulate matter (PM2.5) is associated with cardiovascular diseases. In order to investigate the molecular mechanisms of air pollution-induced CVDs toxicity, human umbilical vein endothelial cells (HUVECs) were exposed to PM2.5 collected from January, 2016 winter in Beijing, China. We performed RNA sequencing to elucidate key molecular mechanism of PM 2.5-mediated toxicity in HUVECs. A total of 1753 genes, 864 up-regulated and 889 down-regulated, were observed to be differentially expressed genes (DEGs). Among these, genes involved in metabolic response, oxidative stress, inflammatory response, and vascular dysfunction were significantly differentially expressed (log2 FC > 4). The results were validated by quantitative real-time PCR (qPCR) and Western blot for CYP1B1, HMOX1, IL8, and GJA4. Pathway analysis revealed that DEGs were involved in the biological processes related to metabolism, inflammation, and host defense against environmental insults. This research is providing a further understanding of the mechanisms underlying PM2.5-induced cardiovascular diseases (CVDs).
Keyword :
5 5 CVDs CVDs RNA-seq RNA-seq PM2 PM2 HUVEC HUVEC
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GB/T 7714 | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis [J]. | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 , 32 (9) : 2052-2064 . |
MLA | Zhou, Zhixiang et al. "Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis" . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH 32 . 9 (2021) : 2052-2064 . |
APA | Zhou, Zhixiang , Qin, Mengnan , Khodahemmati, Sara , Li, Wenke , Niu, Bingyu , Li, Jiangshuai et al. Gene expression in human umbilical vein endothelial cells exposed to fine particulate matter: RNA sequencing analysis . | INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH , 2021 , 32 (9) , 2052-2064 . |
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Abstract :
Antibiotic resistance genes (ARGs) were considered as emerging genetic contaminants, which were threatening public health worldwide. ARGs had two forms of existence: intracellular ARGs (i-ARGs) and extracellular ARGs (e-ARGs), and their emergence and spread patterns were rarely revealed in aerobic granular sludge (AGS) system. The results showed that both i-ARGs and e-ARGs were enriched but had different shift patterns during AGS cultivation process, and e-ARGs had higher growth rate. Most functional bacteria enriched in AGS might play important roles in accumulating of i/e-ARGs, and their oligotypes might be the key reason for the different correlations between taxa and i/e-ARGs. E-ARGs might be transformed from i-ARGs and mainly carried by eintegrons, and three major modules of co-occurrence among e-ARGs were observed. The combined action of environmental factors and bacterial community contributed most (35.43%) to the shift pattern of e-ARGs. This study deciphered the migrations and accumulations of i/e-ARGs during aerobic granulation process, and provided several meaningful comprehensions on i/e-ARGs dissemination during practical AGS application.
Keyword :
I I Aerobic granulation process Aerobic granulation process Oligotypes Oligotypes Functional bacteria Functional bacteria e-antibiotic resistance genes e-antibiotic resistance genes Co-occurrence modules Co-occurrence modules
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GB/T 7714 | Li, Dingchang , Gao, Jingfeng , Dai, Huihui et al. Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process [J]. | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
MLA | Li, Dingchang et al. "Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process" . | CHEMICAL ENGINEERING JOURNAL 421 (2021) . |
APA | Li, Dingchang , Gao, Jingfeng , Dai, Huihui , Duan, Wanjun , Wang, Zhiqi , Zhou, Zhixiang . Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process . | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
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Abstract :
在课程中融入思政教育是实现"三全育人"的关键途径.拓宽创新性教育的视野,结合工科院校分子肿瘤学课程的特点和优势,深层次挖掘思政元素,通过修订课程教学目标、创新教学模式、改革考核评价机制,将思政教育引入课堂教学中,实现知识传授与价值引领的有机统一,为培养和引导研究生树立正确的人生观和价值观奠定了基础.
Keyword :
教学改革 教学改革 课程思政 课程思政 分子肿瘤学 分子肿瘤学 案例教学 案例教学
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GB/T 7714 | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 [J]. | 现代职业教育 , 2021 , (42) : 20-21 . |
MLA | 张芳 et al. "新工科背景下研究生课程分子肿瘤学思政建设初探" . | 现代职业教育 42 (2021) : 20-21 . |
APA | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 . | 现代职业教育 , 2021 , (42) , 20-21 . |
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Abstract :
为了进一步揭示PM_(2.5)暴露对肺的毒性损伤作用,本工作采用16HBE人肺支气管细胞,检测了PM_(2.5)暴露后16HBE的细胞活性、细胞中铁含量、GSH含量、LPO和MDA的产生情况.结果显示,PM_(2.5)(200μg·mL~(-1))处理16HBE细胞24 h后可导致细胞存活率下降、细胞铁含量增加、细胞内LPO和MDA生成量增加、GSH含量降低,而铁死亡抑制剂DFOM (6.25μmol·L~(-1))及Fer-1 (12.50μmol·L~(-1))可以显著减轻PM_(2.5)对细胞的毒性损伤作用,抑制MDA的产生,减少GSH的损耗.透射电子显微镜的形态学观察显示,PM_(2....
Keyword :
人支气管上皮细胞 人支气管上皮细胞 脂质过氧化 脂质过氧化 PM_(2.5) PM_(2.5) 铁死亡 铁死亡
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GB/T 7714 | 冯董董 , 洪启浩 , 李疆帅 et al. PM_(2.5)暴露诱发人支气管上皮细胞16HBE铁死亡 [J]. | 环境科学学报 , 2021 , 41 (09) : 3857-3864 . |
MLA | 冯董董 et al. "PM_(2.5)暴露诱发人支气管上皮细胞16HBE铁死亡" . | 环境科学学报 41 . 09 (2021) : 3857-3864 . |
APA | 冯董董 , 洪启浩 , 李疆帅 , 李文可 , 牛冰羽 , 王碧琪 et al. PM_(2.5)暴露诱发人支气管上皮细胞16HBE铁死亡 . | 环境科学学报 , 2021 , 41 (09) , 3857-3864 . |
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Abstract :
To evaluate the safety and effectiveness of a vaccine based on latent membrane protein 2 (LMP2) modified dendritic cells (DCs) that boosts specific responses of cytotoxic T lymphocytes (CTLs) to LMP2 before and after intradermal injection in patients with nasopharyngeal carcinoma (NPC).DCs were derived from peripheral blood monocytes of patients with NPC. We prepared LMP2-DCs infected by recombinant adenovirus vector expressing LMP2 (rAd-LMP2). NPC patients were immunized with 2 × 10 5 LMP2-DCs by intradermal injection at week 0 and after the second and fourth weeks. Specific responses to LMP2 were detected by enzyme-linked immunospot (ELISPOT) assay at week 0 and at the fifth and eighth weeks. Local clinicians performed the follow-up and tracking of patients.We demonstrated that DCs derived from monocytes displayed typical DC morphologies; the expression of LMP2 in the LMP2-DCs vaccine was confirmed by immunocytochemical assay. Twenty-nine patients with NPC were enrolled in this clinical trial. The LMP2-DCs vaccine was well tolerated in all of the patients. Boosted responses to LMP2 peptide sub-pools were observed in 18 of the 29 patients with NPC. The follow-up data of 29 immunized patients from April, 2010 to April 2015 indicated a five-year survival rate of 94.4% in responders and 45.5% in non-responders.In this pilot study, we demonstrated that the LMP2-DCs vaccine is safe and effective in patients with NPC. Specific CTLs responses to LMP2 play a certain role in controlling and preventing the recurrence and metastasis of NPC, which warrants further clinical testing.
Keyword :
Nasopharyngeal carcinoma Nasopharyngeal carcinoma Immunotherapy Immunotherapy LMP2- DCs LMP2- DCs
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GB/T 7714 | Zeng Yi , Si Yong Feng , Lan Gui Ping et al. LMP2-DC Vaccine Elicits Specific EBV-LMP2 Response to Effectively Improve Immunotherapy in Patients with Nasopharyngeal Cancer. [J]. | Biomedical and environmental sciences : BES , 2020 , 33 (11) : 849-856 . |
MLA | Zeng Yi et al. "LMP2-DC Vaccine Elicits Specific EBV-LMP2 Response to Effectively Improve Immunotherapy in Patients with Nasopharyngeal Cancer." . | Biomedical and environmental sciences : BES 33 . 11 (2020) : 849-856 . |
APA | Zeng Yi , Si Yong Feng , Lan Gui Ping , Wang Zhan , Zhou Ling , Tang Min Zhong et al. LMP2-DC Vaccine Elicits Specific EBV-LMP2 Response to Effectively Improve Immunotherapy in Patients with Nasopharyngeal Cancer. . | Biomedical and environmental sciences : BES , 2020 , 33 (11) , 849-856 . |
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