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学者姓名：周志祥
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Abstract ：
目的 探究巨噬细胞在细颗粒物(fine particulate matter, PM_(2.5))暴露肺泡气血屏障损伤过程中的作用。方法 将18只10周龄、体质量24～27 g的雄性BALB/C小鼠随机分为(n=6):对照组(气管滴注生理盐水)、PM_(2.5)低剂量组与PM_(2.5)高剂量组(在实验第1、4、7天气管滴注PM_(2.5)样品悬液，染毒浓度分别为1.8和16.2 mg/kg体质量),末次染毒24 h后检测PM_(2.5)暴露后小鼠肺组织病理改变、支气管肺泡灌洗液(bronchoalveolar lavage fluid, BALF)中总蛋白(total protein, TP)、乳酸脱氢酶(lactate dehydrogenase, LDH)和碱性磷酸酶(alkaline phosphatase, AKP)释放水平以及肺组织中F4/80蛋白表达水平，观察小鼠肺泡气血屏障的损伤效应及肺组织中巨噬细胞的浸润情况。以肺泡上皮细胞A549和血管内皮细胞EA.hy926构建体外肺泡气血屏障模型，结合THP-1巨噬细胞模型，将PM_(2.5)上清、巨噬细胞培养上清和PM_(2.5)-巨噬细胞培养上清分别与屏障模型孵育24 h,通过检测屏障模型跨膜电阻值(transepithelial electrical resistance, TEER)、荧光素钠透过率和屏障细胞LDH释放情况，确认PM_(2.5)暴露诱导屏障受损过程中巨噬细胞的促进作用；实时荧光定量PCR(quantitative real-time PCR,RT-qPCR)和酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)检测PM_(2.5)暴露后巨噬细胞炎性细胞因子肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、IL-6和IL-8的表达情况，探究巨噬细胞在PM_(2.5)暴露中炎性应激效应。结果 PM_(2.5)暴露可引起小鼠肺组织损伤，且随染毒剂量增加，BALF中TP、LDH、AKP含量及肺组织中巨噬细胞数量随之增加，PM_(2.5)高剂量组与对照组相比具有显著差异(P<0.01)。体外肺泡气血屏障模型暴露结果显示：与150、300μg/mL PM_(2.5)上清或巨噬细胞培养上清单独作用于屏障模型相...
Keyword ：
PM_(2.5) PM_(2.5) 巨噬细胞 巨噬细胞 气血屏障 气血屏障 肺泡 肺泡
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| GB/T 7714 | 姚梦菲 , 王国镇 , 侯潇楠 et al. 巨噬细胞在PM_(2.5)暴露肺泡气血屏障损伤过程中的作用研究 [J]. | 陆军军医大学学报 , 2024 , 46 (08) : 849-858 . |
| MLA | 姚梦菲 et al. "巨噬细胞在PM_(2.5)暴露肺泡气血屏障损伤过程中的作用研究" . | 陆军军医大学学报 46 . 08 (2024) : 849-858 . |
| APA | 姚梦菲 , 王国镇 , 侯潇楠 , 唐铎 , 刘紫佳 , 盛超 et al. 巨噬细胞在PM_(2.5)暴露肺泡气血屏障损伤过程中的作用研究 . | 陆军军医大学学报 , 2024 , 46 (08) , 849-858 . |
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Abstract ：
Background Gout is a common inflammatory arthritis, which is mainly caused by the deposition of monosodium urate (MSU) in tissues. Transcriptomics was used to explore the pathogenesis and treatment of gout in our work.Objective The objective of the study was to analyze and validate potential therapeutic targets and biomarkers in THP-1 cells that were exposed to MSU.Methods THP-1 cells were exposed to MSU. The inflammatory effect was characterized, and RNA-Seq analysis was then carried out. The differential genes obtained by RNA-Seq were analyzed with gene expression omnibus (GEO) series 160170 (GSE160170) gout-related clinical samples in the GEO database and gout-related genes in the GeneCards database. From the three analysis approaches, the genes with significant differences were verified by the differential genes' transcription levels. The interaction relationship of long non-coding RNA (lncRNA) was proposed by ceRNA network analysis.Results MSU significantly promoted the release of IL-1 beta and IL-18 in THP-1 cells, which aggravated their inflammatory effect. Through RNA-Seq, 698 differential genes were obtained, including 606 differential mRNA and 92 differential `LncRNA. Cross-analysis of the RNA-Seq differential genes, the GSE160170 differential genes, and the gout-related genes in GeneCards revealed a total of 17 genes coexisting in the tripartite data. Furthermore, seven differential genes-C-X-C motif chemokine ligand 8 (CXCL8), C-X-C motif chemokine ligand 2 (CXCL2), tumor necrosis factor (TNF), C-C motif chemokine ligand 3 (CCL3), suppressor of cytokine signaling 3 (SOCS3), oncostatin M (OSM), and MIR22 host gene (MIR22HG)-were verified as key genes that analyzed the weight of genes in pathways, the enrichment of inflammation-related pathways, and protein-protein interaction (PPI) nodes combined with the expression of genes in RNA-Seq and GSE160170. It is suggested that MIR22HG may regulate OSM and SOCS3 through microRNA 4271 (miR-4271), OSM, and SOCS3m; CCL3 through microRNA 149-3p (miR-149-3p); and CXCL2 through microRNA 4652-3p (miR-4652-3p).Conclusion The potential of CXCL8, CXCL2, TNF, CCL3, SOCS3, and OSM as gout biomarkers and MIR22HG as a therapeutic target for gout are proposed, which provide new insights into the mechanisms of gout biomarkers and therapeutic methods.
Keyword ：
Gouty arthritis Gouty arthritis monosodium urate monosodium urate protein-protein interaction protein-protein interaction transcriptomic analysis transcriptomic analysis biomarker biomarker competitive endogenous RNA competitive endogenous RNA
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| GB/T 7714 | Wang, Guozhen , Liu, Zijia , Zheng, Yuchen et al. Transcriptomic Analysis of THP-1 Cells Exposed by Monosodium Urate Reveals Key Genes Involved in Gout [J]. | COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING , 2024 , 27 (18) : 2741-2752 . |
| MLA | Wang, Guozhen et al. "Transcriptomic Analysis of THP-1 Cells Exposed by Monosodium Urate Reveals Key Genes Involved in Gout" . | COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING 27 . 18 (2024) : 2741-2752 . |
| APA | Wang, Guozhen , Liu, Zijia , Zheng, Yuchen , Sheng, Chao , Hou, Xiaonan , Yao, Mengfei et al. Transcriptomic Analysis of THP-1 Cells Exposed by Monosodium Urate Reveals Key Genes Involved in Gout . | COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING , 2024 , 27 (18) , 2741-2752 . |
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Abstract ：
Human papillomavirus (HPV) infection is one of the main causes of esophageal carcinoma (ESCA), and its carcinogenic mechanisms in ESCA require further investigation. E6 and E7 are HPV oncogenes, and their genomic integration is a crucial reason for the transformation of host cells into cancer cells. In order to reveal the role of oncogenes E6 and E7 in ESCA cells, the RNA-Seq raw data for HPV18-positive and -negative esophageal squamous cell carcinoma (ESCC) samples derived from the NCBI BioProject database were analyzed, and the differentially expressed genes were identified. Moreover, differentially expressed genes were enriched significantly in multiple cell death pathways, including apoptosis (cyclin-dependent kinase inhibitor 2A, plakophilin 1 and desmoglein 3), pyroptosis (gasdermin A, gasdermin C, NLR family pyrin domain containing 3, absent in melanoma 2, NLR family pyrin domain containing 1 and Toll like receptor 1) and autophagy (Unc-51 like autophagy activating kinase 1, adrenoceptor beta 2). Consequently, the effects of cisplatin-induced apoptosis and Hank's balanced salt solution-induced autophagy, and alpha-ketoglutarate-induced pyroptosis in the ESCC-expressing E6 and E7 cells were verified. Therefore, the expression of E6E7 may culminate in the inhibition of multiple cell death modes, which may also be one of the mechanisms of oncogene-induced carcinogenesis.
Keyword ：
esophageal squamous cell carcinoma esophageal squamous cell carcinoma regulated cell death regulated cell death transcriptomic transcriptomic human papilloma virus 18 human papilloma virus 18
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| GB/T 7714 | Tang, Duo , Wang, Guozhen , Liu, Zijia et al. Transcriptomic analysis of the effects of the HPV18 E6E7 gene on the cell death mode in esophageal squamous cell carcinoma [J]. | ONCOLOGY LETTERS , 2023 , 25 (4) . |
| MLA | Tang, Duo et al. "Transcriptomic analysis of the effects of the HPV18 E6E7 gene on the cell death mode in esophageal squamous cell carcinoma" . | ONCOLOGY LETTERS 25 . 4 (2023) . |
| APA | Tang, Duo , Wang, Guozhen , Liu, Zijia , Wang, Biqi , Yao, Mengfei , Wang, Qian et al. Transcriptomic analysis of the effects of the HPV18 E6E7 gene on the cell death mode in esophageal squamous cell carcinoma . | ONCOLOGY LETTERS , 2023 , 25 (4) . |
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Abstract ：
Oncogene E6 plays a critical role in the development and progression of esophageal cancer caused by human papillomavirus (HPV) infection. Alpha-ketoglutarate (AKG) is a key metabolite in the tricarboxylic acid cycle and has been widely used as a dietary and anti-ageing supplement. In this study, we found that treating esophageal squamous carcinoma cells with a high dose of AKG can induce cell pyroptosis. Furthermore, our research confirms that HPV18 E6 inhibits AKG-induced pyroptosis of esophageal squamous carcinoma cells by lowering P53 expression. P53 downregulates malate dehydrogenase 1 (MDH1) expression; however, MDH1 downregulates L-2-hydroxyglutarate (L-2HG) expression, which inhibits a rise in reactive oxygen species (ROS) levels-as L-2HG is responsible for excessive ROS. This study reveals the actuating mechanism behind cell pyroptosis of esophageal squamous carcinoma cells induced by high concentrations of AKG, and we posit the molecular pathway via which the HPV E6 oncoprotein inhibits cell pyroptosis.
Keyword ：
pyroptosis pyroptosis ROS ROS & alpha;-ketoglutarate & alpha;-ketoglutarate GSDMC GSDMC E6 oncogene E6 oncogene HPV HPV
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| GB/T 7714 | Tang, Duo , Zheng, Yuchen , Wang, Guozhen et al. HPV18 E6 inhibits & alpha;-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway [J]. | FEBS OPEN BIO , 2023 , 13 (8) : 1522-1535 . |
| MLA | Tang, Duo et al. "HPV18 E6 inhibits & alpha;-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway" . | FEBS OPEN BIO 13 . 8 (2023) : 1522-1535 . |
| APA | Tang, Duo , Zheng, Yuchen , Wang, Guozhen , Sheng, Chao , Liu, Zijia , Wang, Biqi et al. HPV18 E6 inhibits & alpha;-ketoglutarate-induced pyroptosis of esophageal squamous cell carcinoma cells via the P53/MDH1/ROS/GSDMC pathway . | FEBS OPEN BIO , 2023 , 13 (8) , 1522-1535 . |
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Abstract ：
大气细颗粒物（PM_(2.5)）作为空气污染的主要污染物，由于其具有颗粒小、比表面积大的特点，很容易沉积于肺泡细胞或进入血流.现有的研究报道指出，许多呼吸道疾病都与暴露于环境PM_(2.5)有关.肺泡巨噬细胞作为一种固有免疫细胞，是抵御外来污染物和致病微生物的第一道防线.本研究以THP-1细胞为人巨噬细胞模型，探究了PM_(2.5)暴露引起巨噬细胞发生炎症反应的机制.通过对暴露于PM_(2.5)后的THP-1细胞进行RNA-Seq测序，筛选出3967个差异表达基因，对这些差异表达基因进行GO分析、KEGG富集分析，结果显示，多条通路与免疫反应及炎症信号有关.通过对230个富集于炎症相关通路的差异表达基因进行蛋白质-蛋白质相互作用分析，结果显示，CXCL8(C-X-C motif chemokine ligand 8）在蛋白质相互作用中处于核心地位.因此，进一步利用实时荧光定量PCR和ELISA对PM_(2.5)暴露的THP-1细胞中的CXCL8表达水平进行验证，发现在低浓度PM_(2.5)暴露的前24 h以内，CXCL8的相对转录水平及分泌水平随着PM_(2.5)暴露时间的延长和暴露浓度的增加而升高.研究表明，CXCL8在PM_(2.5)暴露的THP-1细胞中表达显著，且处于通路蛋白互作的核心位置，提示CXCL8在PM_(2.5)暴露引起的肺部炎症反应中发挥着重要作用.
Keyword ：
RNA-seq RNA-seq THP-1细胞 THP-1细胞 炎症应激 炎症应激 PM_(2.5) PM_(2.5) CXCL8 CXCL8
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| GB/T 7714 | 张雨晨 , 王国镇 , 洪启浩 et al. 基于转录组测序探究PM_(2.5)引起THP-1细胞发生炎性反应的关键因子 [J]. | 环境科学学报 , 2023 , 43 (08) : 454-463 . |
| MLA | 张雨晨 et al. "基于转录组测序探究PM_(2.5)引起THP-1细胞发生炎性反应的关键因子" . | 环境科学学报 43 . 08 (2023) : 454-463 . |
| APA | 张雨晨 , 王国镇 , 洪启浩 , 刘紫佳 , 郑雨晨 , 唐铎 et al. 基于转录组测序探究PM_(2.5)引起THP-1细胞发生炎性反应的关键因子 . | 环境科学学报 , 2023 , 43 (08) , 454-463 . |
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Abstract ：
Objective To establish a sensitive, simple and rapid detection method for African swine fever virus (ASFV) B646L gene. Methods A recombinase-aided amplification-lateral flow dipstick (RAA-LFD) assay was developed in this study. Recombinase-aided amplification (RAA) is used to amplify template DNA, and lateral flow dipstick (LFD) is used to interpret the results after the amplification is completed. The lower limits of detection and specificity of the RAA assay were verified using recombinant plasmid and pathogenic nucleic acid. In addition, 30 clinical samples were tested to evaluate the performance of the RAA assay. Results The RAA-LFD assay was completed within 15 min at 37 degrees C, including 10 min for nucleic acid amplification and 5 minutes for LFD reading results. The detection limit of this assay was found to be 200 copies per reaction. And there was no cross-reactivity with other swine viruses. Conclusion A highly sensitive, specific, and simple RAA-LFD method was developed for the rapid detection of the ASFV.
Keyword ：
Lateral flow detection Lateral flow detection African swine fever virus African swine fever virus Recombinase aided amplification Recombinase aided amplification
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| GB/T 7714 | Li Jiang Shuai , Hao Yan Zhe , Hou Mei Ling et al. Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus [J]. | BIOMEDICAL AND ENVIRONMENTAL SCIENCES , 2022 , 35 (2) : 133-140 . |
| MLA | Li Jiang Shuai et al. "Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus" . | BIOMEDICAL AND ENVIRONMENTAL SCIENCES 35 . 2 (2022) : 133-140 . |
| APA | Li Jiang Shuai , Hao Yan Zhe , Hou Mei Ling , Zhang Xuan , Zhang Xiao Guang , Cao Yu Xi et al. Development of a Recombinase-aided Amplification Combined With Lateral Flow Dipstick Assay for the Rapid Detection of the African Swine Fever Virus . | BIOMEDICAL AND ENVIRONMENTAL SCIENCES , 2022 , 35 (2) , 133-140 . |
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Abstract ：
A random laser carrying the scattering information on a biological host is a promising tool for the characterization of biophysical properties. In this work, random lasing from label-free living cells is proposed to achieve rapid cytometry of apoptosis. Random lasing is achieved by adding biocompatible gain medium to a confocal dish containing cells under optically pumped conditions. The random lasing characteristics are distinct at different stages of cell apoptosis after drug treatment. By analyzing the power Fourier transform results of the random lasing spectra, the percentage of apoptotic cells could be distinguished within two seconds, which is more than an order of magnitude faster than traditional flow cytometry. These results provide a label-free approach for rapid cytometry of apoptosis, which is advantageous for further research of random lasers in the biological field.
Keyword ：
cytometry cytometry living cells living cells random lasing random lasing label-free label-free apoptosis apoptosis
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| GB/T 7714 | Xu, Zhiyang , Hong, Qihao , Ge, Kun et al. Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis [J]. | NANO LETTERS , 2022 , 22 (1) : 172-178 . |
| MLA | Xu, Zhiyang et al. "Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis" . | NANO LETTERS 22 . 1 (2022) : 172-178 . |
| APA | Xu, Zhiyang , Hong, Qihao , Ge, Kun , Shi, Xiaoyu , Wang, Xiaolei , Deng, Jinxiang et al. Random Lasing from Label-Free Living Cells for Rapid Cytometry of Apoptosis . | NANO LETTERS , 2022 , 22 (1) , 172-178 . |
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Abstract ：
为了进一步揭示PM_(2.5)暴露对肺的毒性损伤作用,本工作采用16HBE人肺支气管细胞,检测了PM_(2.5)暴露后16HBE的细胞活性、细胞中铁含量、GSH含量、LPO和MDA的产生情况.结果显示,PM_(2.5)(200μg·mL~(-1))处理16HBE细胞24 h后可导致细胞存活率下降、细胞铁含量增加、细胞内LPO和MDA生成量增加、GSH含量降低,而铁死亡抑制剂DFOM (6.25μmol·L~(-1))及Fer-1 (12.50μmol·L~(-1))可以显著减轻PM_(2.5)对细胞的毒性损伤作用,抑制MDA的产生,减少GSH的损耗.透射电子显微镜的形态学观察显示,PM_(2....
Keyword ：
人支气管上皮细胞 人支气管上皮细胞 脂质过氧化 脂质过氧化 PM_(2.5) PM_(2.5) 铁死亡 铁死亡
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| GB/T 7714 | 冯董董 , 洪启浩 , 李疆帅 et al. PM_(2.5)暴露诱发人支气管上皮细胞16HBE铁死亡 [J]. | 环境科学学报 , 2021 , 41 (09) : 3857-3864 . |
| MLA | 冯董董 et al. "PM_(2.5)暴露诱发人支气管上皮细胞16HBE铁死亡" . | 环境科学学报 41 . 09 (2021) : 3857-3864 . |
| APA | 冯董董 , 洪启浩 , 李疆帅 , 李文可 , 牛冰羽 , 王碧琪 et al. PM_(2.5)暴露诱发人支气管上皮细胞16HBE铁死亡 . | 环境科学学报 , 2021 , 41 (09) , 3857-3864 . |
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Abstract ：
在课程中融入思政教育是实现"三全育人"的关键途径.拓宽创新性教育的视野,结合工科院校分子肿瘤学课程的特点和优势,深层次挖掘思政元素,通过修订课程教学目标、创新教学模式、改革考核评价机制,将思政教育引入课堂教学中,实现知识传授与价值引领的有机统一,为培养和引导研究生树立正确的人生观和价值观奠定了基础.
Keyword ：
教学改革 教学改革 课程思政 课程思政 分子肿瘤学 分子肿瘤学 案例教学 案例教学
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| GB/T 7714 | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 [J]. | 现代职业教育 , 2021 , (42) : 20-21 . |
| MLA | 张芳 et al. "新工科背景下研究生课程分子肿瘤学思政建设初探" . | 现代职业教育 42 (2021) : 20-21 . |
| APA | 张芳 , 周志祥 . 新工科背景下研究生课程分子肿瘤学思政建设初探 . | 现代职业教育 , 2021 , (42) , 20-21 . |
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Abstract ：
Antibiotic resistance genes (ARGs) were considered as emerging genetic contaminants, which were threatening public health worldwide. ARGs had two forms of existence: intracellular ARGs (i-ARGs) and extracellular ARGs (e-ARGs), and their emergence and spread patterns were rarely revealed in aerobic granular sludge (AGS) system. The results showed that both i-ARGs and e-ARGs were enriched but had different shift patterns during AGS cultivation process, and e-ARGs had higher growth rate. Most functional bacteria enriched in AGS might play important roles in accumulating of i/e-ARGs, and their oligotypes might be the key reason for the different correlations between taxa and i/e-ARGs. E-ARGs might be transformed from i-ARGs and mainly carried by eintegrons, and three major modules of co-occurrence among e-ARGs were observed. The combined action of environmental factors and bacterial community contributed most (35.43%) to the shift pattern of e-ARGs. This study deciphered the migrations and accumulations of i/e-ARGs during aerobic granulation process, and provided several meaningful comprehensions on i/e-ARGs dissemination during practical AGS application.
Keyword ：
I I Aerobic granulation process Aerobic granulation process Oligotypes Oligotypes Functional bacteria Functional bacteria e-antibiotic resistance genes e-antibiotic resistance genes Co-occurrence modules Co-occurrence modules
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| GB/T 7714 | Li, Dingchang , Gao, Jingfeng , Dai, Huihui et al. Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process [J]. | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
| MLA | Li, Dingchang et al. "Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process" . | CHEMICAL ENGINEERING JOURNAL 421 (2021) . |
| APA | Li, Dingchang , Gao, Jingfeng , Dai, Huihui , Duan, Wanjun , Wang, Zhiqi , Zhou, Zhixiang . Fates of intracellular and extracellular antibiotic resistance genes during a pilot-scale aerobic granular sludge cultivation process . | CHEMICAL ENGINEERING JOURNAL , 2021 , 421 . |
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