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学者姓名:汪夏燕
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Abstract :
Carbon-centered radicals are key reactive radical intermediates existing in the environment and organisms. The detection of carbon-centered radicals is important for understanding the mechanisms of radical transformations in these fields. In recent years, methods for detecting carbon-centered radicals using bifunctional fluorescent probes have been developed. This review summarized the research progresses of carbon-centered radicals fluorescent probes from the aspects of fluorophores, radical trapping groups and their application fields. Their development and future application were prospected.
Keyword :
Review Review Fluorescent probe Fluorescent probe Radical trapping Radical trapping Carbon-centered radicals Carbon-centered radicals
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GB/T 7714 | Diao, Rui , Dou, Xiang-nan , Wang, Xia-yan . Research Progress of Carbon-Centered Radicals Fluorescent Probes [J]. | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2023 , 51 (7) : 1067-1076 . |
MLA | Diao, Rui 等. "Research Progress of Carbon-Centered Radicals Fluorescent Probes" . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 51 . 7 (2023) : 1067-1076 . |
APA | Diao, Rui , Dou, Xiang-nan , Wang, Xia-yan . Research Progress of Carbon-Centered Radicals Fluorescent Probes . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2023 , 51 (7) , 1067-1076 . |
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Single-cell analysis is important for early diagnosis and treatment of major diseases, drug screening, and studying physiopathological processes. Microfluidic chips are capable of precisely controlling the microenvironment of single cells and monitoring their behavior in real-time, and have become a powerful tool for single-cell analysis. Single-cell capture is an important step in single-cell analysis. Till now, several microfluidic-chip-based single-cell capture methods have been reported, among which hydrodynamic microfluidic- chip-based single-cell capture has the advantages such as easy operation and high-efficiency of single-cell capture, and thus has received wide attention and has been used by researchers. To comprehensively understand the research status of hydrodynamic microfluidic-chip-based single-cell capture, master the structural design of high -efficiency single-cell capture microfluidic chips, and realize the accurate and rapid analysis of single cells, in this paper, the principle of efficient single-cell capture based on hydrodynamics and the structure of microfluidic chips are reviewed. There are three types of structures according to the structural design including micro-well structures, microcolumn structures and bypass channel structures. The optimization process of single-cell capture microfluidic chips is introduced. The materials, structural features, and single-cell capture efficiency of microfluidic chips are summarized, and the advantages and shortcomings of each single-cell capture structure are analyzed. Finally, the development trend of the hydrodynamic-based microfluidic chip single-cell capture method is prospected.
Keyword :
Capture Capture Microfluidic chip Microfluidic chip Hydrodynamics Hydrodynamics Review Review Single-cell Single-cell
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GB/T 7714 | Pan Ting , Wu Yuan-Yuan , Guo Guang-Sheng et al. Advances in Microfluidic Chip Structures Based on Hydrodynamics of Efficient Single-Cell Capture [J]. | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2023 , 51 (6) : 934-944 . |
MLA | Pan Ting et al. "Advances in Microfluidic Chip Structures Based on Hydrodynamics of Efficient Single-Cell Capture" . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 51 . 6 (2023) : 934-944 . |
APA | Pan Ting , Wu Yuan-Yuan , Guo Guang-Sheng , Wang Xia-Yan . Advances in Microfluidic Chip Structures Based on Hydrodynamics of Efficient Single-Cell Capture . | CHINESE JOURNAL OF ANALYTICAL CHEMISTRY , 2023 , 51 (6) , 934-944 . |
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Abstract :
The nucleation stage plays a decisive role in determining nanocrystal morphology and properties; hence, the ability to regulate nucleation is critical for achieving high-level control. Herein, glass microfluidic chips with S-shaped mixing units are designed for the synthesis of Au@Pt core/shell materials. The use of hydrodynamics to tune the nucleation kinetics is explored by varying the number of mixing units. Dendritic Au@Pt core/shell nanomaterials are controllably synthesized and a formation mechanism is proposed. As-synthesized Au@Pt exhibited excellent ethanol oxidation activity under alkaline conditions (8.4 times that of commercial Pt/C). This approach is also successfully applied to the synthesize of Au@Pd core/shell nanomaterials, thus demonstrating its generality. Glass microfluidic chips with S-shaped mixing units are designed to synthesize Au@Pt core/shell materials. By adjusting the number of mixing units, the hydrodynamics is manipulated to modulate the nucleation kinetics, dendritic Au@Pt core/shell nanomaterials are controllably synthesized. The hybrid efficiency dictates the initial nucleation state and determines the ultimate morphology of the product.image
Keyword :
fluid control fluid control microfluidic synthesis microfluidic synthesis core/shell materials core/shell materials Au@Pt Au@Pt
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GB/T 7714 | Wang, Wubin , Wang, Yacheng , Zhang, Dongtang et al. Kinetically Controlled Nucleation Enabled by Tunable Microfluidic Mixing for the Synthesis of Dendritic Au@Pt Core/Shell Nanomaterials [J]. | SMALL , 2023 . |
MLA | Wang, Wubin et al. "Kinetically Controlled Nucleation Enabled by Tunable Microfluidic Mixing for the Synthesis of Dendritic Au@Pt Core/Shell Nanomaterials" . | SMALL (2023) . |
APA | Wang, Wubin , Wang, Yacheng , Zhang, Dongtang , Guo, Guangsheng , Wang, Leyu , Wang, Xiayan . Kinetically Controlled Nucleation Enabled by Tunable Microfluidic Mixing for the Synthesis of Dendritic Au@Pt Core/Shell Nanomaterials . | SMALL , 2023 . |
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Abstract :
Nanoparticlesare increasingly being used for biological applications,such as drug delivery and gene transfection. Different biologicaland bioinspired building blocks have been used for generating suchparticles, including lipids and synthetic polymers. Proteins are anattractive class of material for such applications due to their excellentbiocompatibility, low immunogenicity, and self-assembly characteristics.Stable, controllable, and homogeneous formation of protein nanoparticles,which is key to successfully delivering cargo intracellularly, hasbeen challenging to achieve using conventional methods. In order toaddress this issue, we employed droplet microfluidics and utilizedthe characteristic of rapid and continuous mixing within microdropletsin order to produce highly monodisperse protein nanoparticles. Weexploit the naturally occurring vortex flows within microdropletsto prevent nanoparticle aggregation following nucleation, resultingin systematic control over the particle size and monodispersity. Throughcombination of simulation and experiment, we find that the internalvortex velocity within microdroplets determines the uniformity ofthe protein nanoparticles, and by varying parameters such as proteinconcentration and flow rates, we are able to finely tune nanoparticledimensional properties. Finally, we show that our nanoparticles arehighly biocompatible with HEK-293 cells, and through confocal microscopy,we determine that the nanoparticles fully enter into the cell withalmost all cells containing them. Due to the high throughput of themethod of production and the level of control afforded, we believethat the approach described in this study for generating monodisperseprotein-based nanoparticles has the potential for intracellular drugdelivery or for gene transfection in the future.
Keyword :
protein nanoparticles protein nanoparticles intracellular delivery intracellular delivery high-throughputnanoparticle formation high-throughputnanoparticle formation dropletmicrofluidics dropletmicrofluidics beta-lactoglobulin beta-lactoglobulin regenerated silk fibroin regenerated silk fibroin Bovine serum albumin Bovine serum albumin
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GB/T 7714 | Zhang, Qi , Toprakcioglu, Zenon , Jayaram, Akhila K. et al. Formation of Protein Nanoparticles in Microdroplet Flow Reactors [J]. | ACS NANO , 2023 , 17 (12) : 11335-11344 . |
MLA | Zhang, Qi et al. "Formation of Protein Nanoparticles in Microdroplet Flow Reactors" . | ACS NANO 17 . 12 (2023) : 11335-11344 . |
APA | Zhang, Qi , Toprakcioglu, Zenon , Jayaram, Akhila K. , Guo, Guangsheng , Wang, Xiayan , Knowles, Tuomas P. J. . Formation of Protein Nanoparticles in Microdroplet Flow Reactors . | ACS NANO , 2023 , 17 (12) , 11335-11344 . |
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Abstract :
The accumulation and spatial distribution of intracellularnanoplasticparticles provide useful information about their spatiotemporal toxicologicaleffects mediated by the physicochemical parameters of nanoplasticsin living cells. In this study, a sample injection-transfer methodwas designed with an accuracy of up to femtoliters to attoliters tomatch the volume required for ultranarrow-bore open-tubular liquidchromatography. The separation and concentration quantification ofmixed polystyrenes in different regions in living cells were achievedby directly transferring picoliter/femtoliter volumes of intracellularcytoplasm to an ultranarrow-bore open-tubular chromatographic column.The measurement of pollutant concentration in different areas of asmall-volume target (single cell) was realized. This method is expectedto be used in the qualitative and quantitative analyses of complex,mixed, and label-free nanoplastics (a few nm in size) in the subregionsof living cells.
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GB/T 7714 | Zhang, Qi , Liu, Yuanxing , Liang, Yingqi et al. Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography [J]. | ANALYTICAL CHEMISTRY , 2023 , 95 (26) : 9739-9745 . |
MLA | Zhang, Qi et al. "Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography" . | ANALYTICAL CHEMISTRY 95 . 26 (2023) : 9739-9745 . |
APA | Zhang, Qi , Liu, Yuanxing , Liang, Yingqi , Wu, Yuanyuan , Zhang, Wenmei , Zhang, Dongtang et al. Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography . | ANALYTICAL CHEMISTRY , 2023 , 95 (26) , 9739-9745 . |
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Abstract :
Noble metal nanoporous materials hold great potential in the field of catalysis, owing to their high open structures and numerous low coordination surface sites. However, the formation of porous nanoparticles is restricted by particle size. Herein, we utilized a Pt1Bi2 intermetallic nanocatalyst to develop a dealloying approach for preparing nanoparticles with a bi-continuous porous and core-shell structure and proposed a mechanism for the formation of pores. The particle size used to form the porous structure can be <10 nm, which enhances the nanocatalyst's performance for the oxygen reduction reaction (ORR). This study provides a new understanding of the formation of porous materials via a dealloying approach.
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GB/T 7714 | Wang, Yacheng , Wang, Wubin , Hu, Wangyan et al. Dealloying of Pt1Bi2 intermetallic toward optimization of electrocatalysis on a Bi-continuous nanoporous core-shell structure [J]. | CHEMICAL COMMUNICATIONS , 2023 , 59 (44) : 6730-6733 . |
MLA | Wang, Yacheng et al. "Dealloying of Pt1Bi2 intermetallic toward optimization of electrocatalysis on a Bi-continuous nanoporous core-shell structure" . | CHEMICAL COMMUNICATIONS 59 . 44 (2023) : 6730-6733 . |
APA | Wang, Yacheng , Wang, Wubin , Hu, Wangyan , Zhang, Dongtang , Guo, Guangsheng , Wang, Xiayan . Dealloying of Pt1Bi2 intermetallic toward optimization of electrocatalysis on a Bi-continuous nanoporous core-shell structure . | CHEMICAL COMMUNICATIONS , 2023 , 59 (44) , 6730-6733 . |
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Abstract :
Studying the mechanisms of drug antitumor activity at the single-cell level can provide information about the responses of cell subpopulations to drug therapy, which is essential for the accurate treatment of cancer. Due to the small size of single cells and the low contents of metabolites, metabolomics-based approaches to studying the mechanisms of drug action at the single-cell level are lacking. Herein, we develop a label-free platform for studying the mechanisms of drug action based on single-cell metabolomics (sMDA-scM) by integrating intact living-cell electro-launching ionization mass spectrometry (ILCEI-MS) with metabolomics analysis. Using this platform, we reveal that non-small-cell lung cancer (NSCLC) cells treated by gefitinib can be clustered into two cell subpopulations with different metabolic responses. The glutathione metabolic pathway of the subpopulation containing 14.4% of the cells is not significantly affected by gefitinib, exhibiting certain resistance characteristics. The presence of these cells masked the judgment of whether cysteine and methionine metabolic pathway was remarkably influenced in the analysis of overall average results, revealing the heterogeneity of the response of single NSCLC cells to gefitinib treatment. The findings provide a basis for evaluating the early therapeutic effects of clinical medicines and insights for overcoming drug resistance in NSCLC subpopulations.
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GB/T 7714 | Zhu, Guizhen , Zhang, Wenmei , Zhao, Yaoyao et al. Single-Cell Metabolomics-Based Strategy for Studying the Mechanisms of Drug Action [J]. | ANALYTICAL CHEMISTRY , 2023 , 95 (10) : 4712-4720 . |
MLA | Zhu, Guizhen et al. "Single-Cell Metabolomics-Based Strategy for Studying the Mechanisms of Drug Action" . | ANALYTICAL CHEMISTRY 95 . 10 (2023) : 4712-4720 . |
APA | Zhu, Guizhen , Zhang, Wenmei , Zhao, Yaoyao , Chen, Tian , Yuan, Hanyu , Liu, Yuanxing et al. Single-Cell Metabolomics-Based Strategy for Studying the Mechanisms of Drug Action . | ANALYTICAL CHEMISTRY , 2023 , 95 (10) , 4712-4720 . |
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Abstract :
Direct oxidation of methane into value-added liquid chemicalsisa promising route for wide applications. However, the efficient conversionof methane with high selectivity under mild conditions is still challenging.Herein, the catalysts of rhodium dispersed on ZSM-5 were synthesizedfor the methane conversion utilizing molecular O-2 as theoxidant together with CO and H2O under a total pressureof 40 bar at <100 & DEG;C. Moreover, the conversion of methaneand the selectivity of formic acid could be improved by increasingacidity over the Rh/ZSM-5 catalysts. Promoted by H+, theactivities of methane oxidation can reach 3.56 mol(HCOOH)/mol(Rh)/h and 23.96 mol(HCOOH)/mol(Rh)/h at 50 and 90 & DEG;C over the 5.0Rh/H-ZSM-5 catalyst, and theselectivity of HCOOH of 98.48% to all organic oxygenates can be acquiredat 90 & DEG;C. Dramatically, the activation of the C-H bondcan even happen at room temperature. Electron paramagnetic resonanceresults showed that acidity can promote the generation of & BULL;OHwith CO, H2O, and O-2 as the reactants, resultingin high catalytic activity under mild conditions.
Keyword :
formic acid formic acid partialoxidation partialoxidation catalyst catalyst rhodium rhodium methane methane
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GB/T 7714 | Gu, Fubo , Qin, Xuetao , Pang, Liu et al. Acid-Promoted Selective Oxidation of Methane to Formic Acid over Dispersed Rhodium Catalysts under Mild Conditions [J]. | ACS CATALYSIS , 2023 , 13 (14) : 9509-9514 . |
MLA | Gu, Fubo et al. "Acid-Promoted Selective Oxidation of Methane to Formic Acid over Dispersed Rhodium Catalysts under Mild Conditions" . | ACS CATALYSIS 13 . 14 (2023) : 9509-9514 . |
APA | Gu, Fubo , Qin, Xuetao , Pang, Liu , Zhang, Ri , Peng, Mi , Xu, Yao et al. Acid-Promoted Selective Oxidation of Methane to Formic Acid over Dispersed Rhodium Catalysts under Mild Conditions . | ACS CATALYSIS , 2023 , 13 (14) , 9509-9514 . |
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Abstract :
Research on single-cell proteomics is required to understand precisely the growth and development of organisms and the onset and progression of diseases. However, research on single-cell proteomics is still in its infancy due in part to the wide variety, low abundance and small amounts of proteins in single cells. Single-cell proteomics based on mass spectrometry (MS) has evolved rapidly over the last two decades due to features such as minimal sample requirements, high sensitivity, rich structural information and the need not to use antibodies for labeling purposes. Electrospray ionization mass spectrometry (ESI-MS) offers the highest protein coverage for single-cell proteomics. However, due to the availability of various pretreatment methods, separation techniques and MS instrumentation, the sample throughput and coverage of single-cell proteomic technologies can vary greatly. In attempting to highlight the recent progress made in the life sciences field, pertinent publications have been classified according to the different methods used for the separation of single-cell protein samples. The performance of the different methods in terms of detection coverage and throughput are introduced, and the technologies affording major breakthroughs and advances are highlighted. The applications and development pros-pects for single-cell proteomics based on ESI-MS are elaborated.(c) 2023 Elsevier B.V. All rights reserved.
Keyword :
Electrospray ionization mass spectrometry Electrospray ionization mass spectrometry Proteomics Proteomics Microfluidic chip Microfluidic chip Capillary electrophoresis Capillary electrophoresis Liquid chromatography Liquid chromatography Single cell Single cell
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GB/T 7714 | Wu, Yuanyuan , Zhang, Wenmei , Zhao, Yaoyao et al. Technology development trend of electrospray ionization mass spectrometry for single-cell proteomics [J]. | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2023 , 159 . |
MLA | Wu, Yuanyuan et al. "Technology development trend of electrospray ionization mass spectrometry for single-cell proteomics" . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY 159 (2023) . |
APA | Wu, Yuanyuan , Zhang, Wenmei , Zhao, Yaoyao , Wang, Xiayan , Guo, Guangsheng . Technology development trend of electrospray ionization mass spectrometry for single-cell proteomics . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2023 , 159 . |
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Abstract :
单细胞分析对于重大疾病的早期诊断及治疗、药物筛选和生理病理过程的研究具有重要意义.微流控芯片能够精确控制单细胞的微环境,实时监测单细胞的行为,已成为单细胞分析的强大工具.单细胞捕获是单细胞分析的重要步骤.目前已报道了多种微流控芯片用于单细胞捕获的方法,其中基于流体动力的微流控芯片单细胞捕获方法具有操作方便、单细胞捕获效率高等优点,受到研究人员的广泛关注及使用.为了全面了解基于流体动力的微流控芯片单细胞捕获方法的研究现状,掌握单细胞高效捕获的微流控芯片结构设计,实现单细胞精准快速分析,本文综述了基于流体动力的单细胞高效捕获(>70%)原理及微流控芯片结构,根据结构设计不同分为微井结构、微柱结构和旁路通道结构,介绍了单细胞高效捕获的微流控芯片优化过程,总结了微流控芯片的材质、结构特点及单细胞捕获效率等,对不同单细胞捕获结构的优势及不足进行了分析.最后,对基于流体动力的微流控芯片单细胞捕获方法的发展趋势进行了展望.
Keyword :
微流控芯片 微流控芯片 流体动力 流体动力 捕获 捕获 评述 评述 单细胞 单细胞
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GB/T 7714 | 潘婷 , 武园园 , 郭广生 et al. 基于流体动力单细胞高效捕获的微流控芯片结构研究进展 [J]. | 分析化学 , 2023 , 51 (6) : 934-944 . |
MLA | 潘婷 et al. "基于流体动力单细胞高效捕获的微流控芯片结构研究进展" . | 分析化学 51 . 6 (2023) : 934-944 . |
APA | 潘婷 , 武园园 , 郭广生 , 汪夏燕 . 基于流体动力单细胞高效捕获的微流控芯片结构研究进展 . | 分析化学 , 2023 , 51 (6) , 934-944 . |
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