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学者姓名：汪夏燕
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Abstract ：
Nucleic acid detection is considered the golden standard for diagnosing infectious diseases caused by various pathogens, including viruses, bacteria, and parasites. PCR and other amplification-based technologies are highly sensitive and specific, allowing for accurate detection and identification of low-level causative pathogens by targeting and amplifying their unique genetic segment (DNA or RNA). However, it is important to recognize that machinery-dependent diagnostic methods may only sometimes be available or practical in resource-limited settings, where direct implementation can be challenging. CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-based diagnostics offer a promising alternative for nucleic acid detection. These methods provide gene sequence-specific targeting, multiplexing capability, rapid result disclosure, and ease of operation, making them suitable for point-of-care (POC) applications. CRISPR-Cas-based nucleic acid detection leverages the intrinsic gene-editing capabilities of CRISPR systems to detect specific DNA or RNA sequences with high precision, ensuring high specificity in identifying pathogens. When integrated with micro- and nano-technologies, CRISPR-based diagnostics gain additional benefits, including automated microfluidic processes, enhanced multiplexed detection, improved sensitivity through nanoparticle integration, and combined detection strategies. In this review, we analyze the motivations for tailoring the CRISPR-Cas system with microfluidic formats or nanoscale materials for nucleic acid biosensing and detection. We discuss and categorize current achievements in such systems, highlighting their differences, commonalities, and opportunities for addressing challenges, particularly for POC diagnostics. Micro- and nano-technologies can significantly enhance the practical utility of the CRISPR-Cas system, enabling more comprehensive diagnostic and surveillance capabilities. By integrating these technologies, CRISPR-based diagnostics can achieve higher levels of automation, sensitivity, and multiplexing, making them invaluable tools in the global effort to diagnose and control infectious diseases.
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| GB/T 7714 | Zhao, Liang , Zhao, Zihao , Li, Ning et al. The nucleic acid detection using CRISPR/Cas biosensing system with micro-nano modality for point-of-care applications [J]. | TALANTA , 2025 , 286 . |
| MLA | Zhao, Liang et al. "The nucleic acid detection using CRISPR/Cas biosensing system with micro-nano modality for point-of-care applications" . | TALANTA 286 (2025) . |
| APA | Zhao, Liang , Zhao, Zihao , Li, Ning , Wang, Xiayan . The nucleic acid detection using CRISPR/Cas biosensing system with micro-nano modality for point-of-care applications . | TALANTA , 2025 , 286 . |
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Abstract ：
Microvasculature-on-a-chip, utilizing microfluidic technology, has emerged as a significant in vitro tool for simulating both the normal and disease states of blood vessel networks. In our review, we highlight the efficacy of microfluidic platforms in accurately reproducing the microenvironment of human blood vessels. We outline a range of methodologies employed to fabricate vascular networks in vitro, , focusing on the use of endothelial cells within microfluidic structures. For each method, we provide an assessment of recent examples, critically evaluating their strengths and drawbacks. Furthermore, we delve into the outlook and the innovative advancements anticipated for next-generation vascular-on-a-chip models and the broader field of chip-based tissue engineering.
Keyword ：
endothelial cells endothelial cells micro-physiological system micro-physiological system microfluidic microfluidic microvasculature networks microvasculature networks
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| GB/T 7714 | Wang, Fangtian , Zhao, Liang , Guo, Guangsheng et al. Microfluidic-Based Vasculatures on Chip: Methods and Recent Progress [J]. | PROGRESS IN CHEMISTRY , 2024 , 36 (6) : 840-850 . |
| MLA | Wang, Fangtian et al. "Microfluidic-Based Vasculatures on Chip: Methods and Recent Progress" . | PROGRESS IN CHEMISTRY 36 . 6 (2024) : 840-850 . |
| APA | Wang, Fangtian , Zhao, Liang , Guo, Guangsheng , Wang, Xiayan . Microfluidic-Based Vasculatures on Chip: Methods and Recent Progress . | PROGRESS IN CHEMISTRY , 2024 , 36 (6) , 840-850 . |
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Abstract ：
MicroRNAs (miRNAs) are a class of endogenous noncoding small RNAs that play important roles in various biological processes and diseases. Direct determination of miRNAs is a cost-efficient and accurate method for analysis. Herein, we established a novel method for the analysis of miRNAs based on a narrow constant-inner-diameter mass spectrometry emitter. We utilized the gravity-assisted sleeving etching method to prepare a constant-inner-diameter mass spectrometry emitter with a capillary inner diameter of 5.5 mu m, coupled it with a high-voltage power supply and a high-resolution mass spectrometer, and used it for miRNA direct detection. The method showed high sensitivity and reproducibility for the analysis of four miRNAs, with a limit of detection of 100 nmol/L (170 amol) for the Hsa-miR-1290 analysis. Compared with commercial ion sources, our method achieved higher sensitivity for miRNA detection. In addition, we analyzed the total miRNAs in the A549 cells. The result indicated that both spiked and endogenous miRNAs could be quantified with high accuracy. As a result, this method offers a promising platform for highly sensitive and accurate miRNA analysis. Furthermore, this approach can be extended to the analysis of other small oligonucleotides and holds the potential for studying clinical samples and facilitating disease diagnosis.
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| GB/T 7714 | Zhang, Wenmei , Zhu, Guizhen , Li, Ning et al. Label-Free Direct Identification of MicroRNAs Based on a Narrow Constant-Inner-Diameter Emitter Mass Spectrometry Analysis [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (22) : 8914-8921 . |
| MLA | Zhang, Wenmei et al. "Label-Free Direct Identification of MicroRNAs Based on a Narrow Constant-Inner-Diameter Emitter Mass Spectrometry Analysis" . | ANALYTICAL CHEMISTRY 96 . 22 (2024) : 8914-8921 . |
| APA | Zhang, Wenmei , Zhu, Guizhen , Li, Ning , Wang, Liangxia , Wang, Mengying , Wu, Yuanyuan et al. Label-Free Direct Identification of MicroRNAs Based on a Narrow Constant-Inner-Diameter Emitter Mass Spectrometry Analysis . | ANALYTICAL CHEMISTRY , 2024 , 96 (22) , 8914-8921 . |
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Abstract ：
Recently, Ni-based chalcogenides havedemonstrated remarkable activity and selectivity for alcohol electrooxidation, but the mechanisms remain debated. This study synthesizes Ni-based electrodeswith different chalcogen anion coordination on nickel nanorod arrays (NiOx/Ni,NiSx/Ni, and NiSex/Ni NRAs). NiSex/Ni NRAsshowcases superior performance (Faradaic efficiency 92.9%) in glycerolelectrooxidation reaction (GOR). In situ spectroscopy reveals that NiSecoordination inhibits deep oxidative reconstruction of the Ni-based interface, preventingNiOOH phase formation during GOR, enhancing activity and stability of NiSex/NiNRAs. Conversely, NiS and NiO coordination lead to deep reconstruction with NiOOHphase formation, limiting GOR performance. Differently, during competingreaction of GOR, the oxygen evolution reaction (OER) leads to deepreconstruction of NiSex interface due to the instability of Ni-Sebonds, inducing performance degradation and dissolution of Se components. Furthermechanism investigation elucidates that the rate-determining step (RDS) ofGOR at the NiSex interface involves oxidation of *C2H3O3 intermediatesthrough H2O adsorption, favoring stable formate production.Contrarily, the RDS at the NiSx, NiOx, and NiOOHinterfaces predominantly focus on the decarboxylation of multi-carbon intermediates, raisingenergy barriers and over-oxidizing formate to CO2. These results providenew insights for designing Ni-based non-oxide catalysts forefficient and stable electrocatalytic oxidation. A free-standing NiSex/Ni nanorod arrays (NiSex/Ni NRAs) electrode with excellent glycerol electrooxidation (GOR) catalytic performance is prepared. In situ spectroscopy and DFT calculations indicate that Ni-Se coordination substantially inhibited the deep oxidative reconstruction of Ni-based interface to form NiOOH phase, and rate-determining step of GOR at NiSex interface is the oxidation of *C2H3O3intermediates through the adsorption of H2O as an oxygen source, these critical factors lead to the superior GOR performance of NiSex/Ni NRAs with efficient and stable formate production. image
Keyword ：
over-oxidation over-oxidation in situ spectroscopic analysis in situ spectroscopic analysis Ni-based chalcogenides Ni-based chalcogenides glycerol electrooxidation glycerol electrooxidation interface reconstruction interface reconstruction
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| GB/T 7714 | Wang, Shuo , Yan, Yong , Du, Yongping et al. Inhibiting the Deep Reconstruction of Ni-Based Interface by Coordination of Chalcogen Anions for Efficient and Stable Glycerol Electrooxidation [J]. | ADVANCED FUNCTIONAL MATERIALS , 2024 , 34 (39) . |
| MLA | Wang, Shuo et al. "Inhibiting the Deep Reconstruction of Ni-Based Interface by Coordination of Chalcogen Anions for Efficient and Stable Glycerol Electrooxidation" . | ADVANCED FUNCTIONAL MATERIALS 34 . 39 (2024) . |
| APA | Wang, Shuo , Yan, Yong , Du, Yongping , Zhao, Yuguo , Li, Tongxian , Wang, Dong et al. Inhibiting the Deep Reconstruction of Ni-Based Interface by Coordination of Chalcogen Anions for Efficient and Stable Glycerol Electrooxidation . | ADVANCED FUNCTIONAL MATERIALS , 2024 , 34 (39) . |
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Abstract ：
Patients with epidermal growth factor receptor mutant nonsmall cell lung cancer (NSCLC) often fail to treat gefitinib because of secondary drug resistance. The development of tumor drug resistance is closely related to variations in cancer cell metabolism. Single-cell metabolomics analysis can provide unique information about tumor drug resistance. Herein, we constructed a platform to study the secondary resistance of tumor cells based on single-cell metabolomics (sSRTC-scM). A gefitinib-resistant NSCLC cell line (PC9GR) was constructed by increasing the dose step by step. The metabolic profiles of parental PC9 cells and PC9GR cells with different drug resistance levels were detected by intact living-cell electrolaunching ionization mass spectrometry at the single-cell level. The data were analyzed by statistical methods such as t-SNE, variance, volcano plot, heat map, and metabolic pathway analysis. Using this platform, we found that the metabolic fingerprints of PC9GR cells can evaluate drug resistance degrees. The metabolic fingerprints continue to be altered with the increase of drug resistance. We revealed 19 metabolic markers of secondary resistance by variance analysis and clarified that the glycerophospholipid metabolic pathway of PC9GR cells changed significantly. In addition, we found that with the increase in drug resistance levels, the heterogeneity of single-cell metabolism became greater and the number of cells with weak drug resistance gradually decreased. This phenomenon can be utilized to illustrate the drug resistance degrees of PC9GR cells. This study provides diagnostic markers for evaluating the drug resistance of tumors and gives new insight into overcoming the secondary resistance of tumors.
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| GB/T 7714 | Zhu, Guizhen , Zhang, Wenmei , Zhao, Yaoyao et al. Single-Cell Mass Spectrometry Studies of Secondary Drug Resistance of Tumor Cells [J]. | ANALYTICAL CHEMISTRY , 2024 , 97 (1) : 337-344 . |
| MLA | Zhu, Guizhen et al. "Single-Cell Mass Spectrometry Studies of Secondary Drug Resistance of Tumor Cells" . | ANALYTICAL CHEMISTRY 97 . 1 (2024) : 337-344 . |
| APA | Zhu, Guizhen , Zhang, Wenmei , Zhao, Yaoyao , Wang, Guangyun , Yuan, Hanyu , Guo, Guangsheng et al. Single-Cell Mass Spectrometry Studies of Secondary Drug Resistance of Tumor Cells . | ANALYTICAL CHEMISTRY , 2024 , 97 (1) , 337-344 . |
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Abstract ：
Understanding the risk trade-offs between nitrogen dioxide (NO2) and ozone (O3) pollution is crucial for ozone governance. The air quality health index (AQHI) provides a more comprehensive measure of air pollution mixtures. This study used environmental, meteorological, and health data of 13 cities in the Beijing-Tianjin-Hebei region for 2018-2020 to assess the health effects of pollutants during both cold and warm seasons. The study reveals that NO2 pollution in the cold season (20.4-63.4 mu g/m3) is more severe compared to warm season (18.3-49.7 mu g/m3), and its concentrations have been decreasing annually in most cities. However, the study also highlights a concerning trend of increasing ozone concentrations during the cold season across all cities in the region (The average annual increase is 3.5 mu g/m3). This increase may be linked to the abatement of nitrogen oxides (NOX) and particulate matter (PM). The health benefit of reducing environmental air NO2 concentrations maybe offset by the increase in O3 concentrations. Emission control measures to reduce nitrogen dioxide, sulfur dioxide, and particulate matter levels have been effective in reducing the negative impacts on health caused by air pollution in various cities in the Beijing-Tianjin-Hebei region. It was necessary to construct the cold season AQHI (AQHI-C) and warm season AQHI (AQHI-W) separately in the Beijing-Tianjin-Hebei region, especially for the assessment of health risks during the cold season.
Keyword ：
Health impact Health impact Nitrogen dioxide Nitrogen dioxide Air quality health index Air quality health index Air pollution Air pollution Ozone pollution Ozone pollution
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| GB/T 7714 | Wang, Yu , Ding, Ding , Dou, Yan et al. Integrated health risk assessment of ozone and nitrogen dioxide pollution during the cold and warm seasons in the Beijing-Tianjin-Hebei region [J]. | AIR QUALITY ATMOSPHERE AND HEALTH , 2024 , 18 (1) : 205-223 . |
| MLA | Wang, Yu et al. "Integrated health risk assessment of ozone and nitrogen dioxide pollution during the cold and warm seasons in the Beijing-Tianjin-Hebei region" . | AIR QUALITY ATMOSPHERE AND HEALTH 18 . 1 (2024) : 205-223 . |
| APA | Wang, Yu , Ding, Ding , Dou, Yan , Xu, Hongbing , Ji, Xiaohui , Xu, Zhizhen et al. Integrated health risk assessment of ozone and nitrogen dioxide pollution during the cold and warm seasons in the Beijing-Tianjin-Hebei region . | AIR QUALITY ATMOSPHERE AND HEALTH , 2024 , 18 (1) , 205-223 . |
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Abstract ：
Endowing current artificial chemical reactions (ACRs) with high specificity and intricate activation capabilities is crucial for expanding their applications in accurate bioimaging within living cells. However, most of the reported ACR-based evaluations relied on either single biomarker stimuli or dual activators without obvious biological relevance, still limiting their accuracy and fidelity. Herein, taking the metal-ion-dependent DNAzyme cleavage reaction as a model ACR, two regulators, glutathione (GSH) and telomerase (TE) activated DNAzyme cleavage reactions, were exploited for precise discrimination of cancerous cells from normal cells. DNA probe was self-assembled into the ZIF-90 nanoparticle framework to construct coordination-driven nanoprobes. This approach enhances the stability and specificity of tumor imaging by utilizing biomarkers associated with rapid tumor proliferation and those commonly overexpressed in tumors. In conclusion, the research not only paves the way for new perspectives in cell biology and pathology studies but also lays a solid foundation for the advancement of biomedical imaging and disease diagnostic technologies.
Keyword ：
tumor proliferation tumor proliferation dual-stimuli dual-stimuli biologicalrelevance biologicalrelevance artificial chemical reactions (ACRs) artificial chemical reactions (ACRs) coordination-driven coordination-driven
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| GB/T 7714 | Ban, Yinbo , Zhou, Fu , Wang, Hao et al. Dual-Stimuli Regulation of DNAzyme Cleavage Reaction by Coordination-Driven Nanoprobes for Cancer Cell Imaging [J]. | ACS APPLIED MATERIALS & INTERFACES , 2024 , 16 (24) : 30766-30775 . |
| MLA | Ban, Yinbo et al. "Dual-Stimuli Regulation of DNAzyme Cleavage Reaction by Coordination-Driven Nanoprobes for Cancer Cell Imaging" . | ACS APPLIED MATERIALS & INTERFACES 16 . 24 (2024) : 30766-30775 . |
| APA | Ban, Yinbo , Zhou, Fu , Wang, Hao , Zhang, Fuqiang , Xia, Mengmeng , Wan, Yifei et al. Dual-Stimuli Regulation of DNAzyme Cleavage Reaction by Coordination-Driven Nanoprobes for Cancer Cell Imaging . | ACS APPLIED MATERIALS & INTERFACES , 2024 , 16 (24) , 30766-30775 . |
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Abstract ：
The nucleation stage plays a decisive role in determining nanocrystal morphology and properties; hence, the ability to regulate nucleation is critical for achieving high-level control. Herein, glass microfluidic chips with S-shaped mixing units are designed for the synthesis of Au@Pt core/shell materials. The use of hydrodynamics to tune the nucleation kinetics is explored by varying the number of mixing units. Dendritic Au@Pt core/shell nanomaterials are controllably synthesized and a formation mechanism is proposed. As-synthesized Au@Pt exhibited excellent ethanol oxidation activity under alkaline conditions (8.4 times that of commercial Pt/C). This approach is also successfully applied to the synthesize of Au@Pd core/shell nanomaterials, thus demonstrating its generality. Glass microfluidic chips with S-shaped mixing units are designed to synthesize Au@Pt core/shell materials. By adjusting the number of mixing units, the hydrodynamics is manipulated to modulate the nucleation kinetics, dendritic Au@Pt core/shell nanomaterials are controllably synthesized. The hybrid efficiency dictates the initial nucleation state and determines the ultimate morphology of the product.image
Keyword ：
fluid control fluid control microfluidic synthesis microfluidic synthesis core/shell materials core/shell materials Au@Pt Au@Pt
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| GB/T 7714 | Wang, Wubin , Wang, Yacheng , Zhang, Dongtang et al. Kinetically Controlled Nucleation Enabled by Tunable Microfluidic Mixing for the Synthesis of Dendritic Au@Pt Core/Shell Nanomaterials [J]. | SMALL , 2023 , 20 (13) . |
| MLA | Wang, Wubin et al. "Kinetically Controlled Nucleation Enabled by Tunable Microfluidic Mixing for the Synthesis of Dendritic Au@Pt Core/Shell Nanomaterials" . | SMALL 20 . 13 (2023) . |
| APA | Wang, Wubin , Wang, Yacheng , Zhang, Dongtang , Guo, Guangsheng , Wang, Leyu , Wang, Xiayan . Kinetically Controlled Nucleation Enabled by Tunable Microfluidic Mixing for the Synthesis of Dendritic Au@Pt Core/Shell Nanomaterials . | SMALL , 2023 , 20 (13) . |
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Abstract ：
活单细胞分析对于揭示细胞异质性、理解生物学过程和机制以及重大疾病预防和早期诊断具有重要意义.胞内传递是基因编辑、药物递送和免疫疗法等的关键步骤,目前存在多种胞内传递方法,多以群体细胞为研究基础,只能实现单一方向传递,传递效率有限,并且多数细胞活性受到影响.基于纳米尖端结构的活单细胞胞内传递方法能够穿越细胞膜,在膜内外建立通道,物质传递过程中基本不会影响细胞的活性,能够实现双向传递,细胞利用率高,可实现单细胞定位及定量胞内传递.本文综述了基于纳米尖端结构的活单细胞胞内传递方法近年的研究进展.根据纳米尖端结构是否中空,分为实心纳米尖端和空心纳米尖端两类,总结了纳米尖端结构的材质、尺寸及制备方法,讨论了基于纳米尖端结构的活单细胞胞内传递方法的驱动力、原理、优缺点及应用,最后,对基于纳米尖端结构的活单细胞胞内传递方法的未来发展趋势进行了展望.
Keyword ：
评述 评述 纳米结构 纳米结构 单细胞分析 单细胞分析 胞内传递 胞内传递
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| GB/T 7714 | 于素素 , 武园园 , 汪夏燕 et al. 基于纳米尖端结构的活单细胞胞内传递方法研究进展 [J]. | 分析化学 , 2023 , 51 (12) : 1855-1866 . |
| MLA | 于素素 et al. "基于纳米尖端结构的活单细胞胞内传递方法研究进展" . | 分析化学 51 . 12 (2023) : 1855-1866 . |
| APA | 于素素 , 武园园 , 汪夏燕 , 郭广生 . 基于纳米尖端结构的活单细胞胞内传递方法研究进展 . | 分析化学 , 2023 , 51 (12) , 1855-1866 . |
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Abstract ：
Research on single-cell proteomics is required to understand precisely the growth and development of organisms and the onset and progression of diseases. However, research on single-cell proteomics is still in its infancy due in part to the wide variety, low abundance and small amounts of proteins in single cells. Single-cell proteomics based on mass spectrometry (MS) has evolved rapidly over the last two decades due to features such as minimal sample requirements, high sensitivity, rich structural information and the need not to use antibodies for labeling purposes. Electrospray ionization mass spectrometry (ESI-MS) offers the highest protein coverage for single-cell proteomics. However, due to the availability of various pretreatment methods, separation techniques and MS instrumentation, the sample throughput and coverage of single-cell proteomic technologies can vary greatly. In attempting to highlight the recent progress made in the life sciences field, pertinent publications have been classified according to the different methods used for the separation of single-cell protein samples. The performance of the different methods in terms of detection coverage and throughput are introduced, and the technologies affording major breakthroughs and advances are highlighted. The applications and development pros-pects for single-cell proteomics based on ESI-MS are elaborated.(c) 2023 Elsevier B.V. All rights reserved.
Keyword ：
Electrospray ionization mass spectrometry Electrospray ionization mass spectrometry Proteomics Proteomics Microfluidic chip Microfluidic chip Capillary electrophoresis Capillary electrophoresis Liquid chromatography Liquid chromatography Single cell Single cell
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| GB/T 7714 | Wu, Yuanyuan , Zhang, Wenmei , Zhao, Yaoyao et al. Technology development trend of electrospray ionization mass spectrometry for single-cell proteomics [J]. | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2023 , 159 . |
| MLA | Wu, Yuanyuan et al. "Technology development trend of electrospray ionization mass spectrometry for single-cell proteomics" . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY 159 (2023) . |
| APA | Wu, Yuanyuan , Zhang, Wenmei , Zhao, Yaoyao , Wang, Xiayan , Guo, Guangsheng . Technology development trend of electrospray ionization mass spectrometry for single-cell proteomics . | TRAC-TRENDS IN ANALYTICAL CHEMISTRY , 2023 , 159 . |
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