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学者姓名:杨宏
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Abstract :
A two-stage partial nitritation/anammox process based on microbial encapsulation (PN/A-E) was established. The nitrogen removal characteristics of PN/A-E under high and low ammonia nitrogen and after reactivation following a long-term shutdown were comprehensively investigated and compared with anammox granular sludge (AnGS). The stable PN process did not depend on high ammonia nitrogen, and the nitrite accumulation rate reached 95.2 +/- 0.7 %. The overall nitrogen removal rate of encapsulated anammox bacteria was twice that of the AnGS, and it was more tolerant to external interference. Moreover, PN/A-E showed good reactivation performance, and the total nitrogen in the effluent was 10.0 +/- 1.4 mg center dot L-1 when the final hydraulic retention time was 2.18 h. The immobilized fillers support an increase in ammonia-oxidizing bacteria under restricted conditions and were more conducive to the dominance of functional bacteria and the stability of microbial community under low ammonia nitrogen. This study provides a positive method to achieve a reliable PN/A.
Keyword :
Microbial community structure Microbial community structure Anammox Anammox Nitrogen removal Nitrogen removal Partial nitritation Partial nitritation Microbial immobilization Microbial immobilization
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GB/T 7714 | Wang, XiaoTong , Yang, Hong , Wang, JiaWei . Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes [J]. | BIORESOURCE TECHNOLOGY , 2023 , 370 . |
MLA | Wang, XiaoTong 等. "Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes" . | BIORESOURCE TECHNOLOGY 370 (2023) . |
APA | Wang, XiaoTong , Yang, Hong , Wang, JiaWei . Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes . | BIORESOURCE TECHNOLOGY , 2023 , 370 . |
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Abstract :
The present study sought to treat the ion-adsorption type rare earth mine wastewater in a pilot plant system using the nitritation-denitrification process with immobilized biological fillers. Meanwhile, the microbial community structure in the immobilized fillers was analyzed. The ammonia nitrogen removal rate of the nitritation fillers reached 0.43 kg N m−3 d−1 on day 61 (28.1 °C) and the total nitrogen removal rate of the denitrification fillers reached 0.51 kg N m−3 d−1 on day 58 (27.8 °C) under the hydraulic retention time of 3.4 hr and a fillers volume ratio of 8% (v/v). The average NH4+-N and TN removal efficiencies of the nitritation-denitrification process were 96.9% and 96.8%, respectively, during the steady state operation period (day 26–72). The results also indicate that high DO (5.5–6.5 mg L−1) could induce rapid growth of nitrite-oxidizing bacteria (NOB) in the nitritation fillers, even though the initial NOB proportion is extremely low. The optimal operational performance of the denitrification fillers reactor was the COD/(NO2−-N + NO3−-N) ratio of 3.7–4.2. The high-throughput sequencing analysis showed that the functional bacteria in the immobilized fillers could maintain a competitive advantage. The abundance of Nitrosomonas in the nitritation fillers increased from 6.58% to 21.24%, while Thauera and Ottowia were the dominant denitrifying bacteria genera in the denitrification fillers. © 2022 Elsevier B.V.
Keyword :
Denitrification; Immobilized biological fillers; Microbial community structure; Nitritation; Rare earth mine wastewater Denitrification; Immobilized biological fillers; Microbial community structure; Nitritation; Rare earth mine wastewater
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GB/T 7714 | Zou, Z. , Yang, H. , Zhang, S. et al. Nitrogen removal performance and microbial community analysis of immobilized biological fillers in rare earth mine wastewater [J]. | Biochemical Engineering Journal , 2022 , 186 . |
MLA | Zou, Z. et al. "Nitrogen removal performance and microbial community analysis of immobilized biological fillers in rare earth mine wastewater" . | Biochemical Engineering Journal 186 (2022) . |
APA | Zou, Z. , Yang, H. , Zhang, S. , Chi, W. , Wang, X. , Liu, Z. . Nitrogen removal performance and microbial community analysis of immobilized biological fillers in rare earth mine wastewater . | Biochemical Engineering Journal , 2022 , 186 . |
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Abstract :
To overcome the issue of substandard nitrogen and phosphorus discharge, resulting from the low carbon/ni-trogen (C/N) ratio of municipal wastewater, immobilized filler was used to re-hydrolyze the alkaline fermen-tation liquid of waste activate sludge (WAS) as a nitrogen removing carbon source. The complex organic matter was further hydrolyzed and its denitrification efficiency as a carbon source for nitrogen removal was improved. Volatile fatty acids increased from 1605 & PLUSMN; 5 mg/L to 2546 +/- 37 mg/L after re-hydrolysis of mixed-alkali sludge fermentation liquid, and complex organic matter, such as fulvic acids, humic acids, class I aromatic proteins, and soluble microbial products were hydrolyzed into small-molecule organics. When the re-hydrolysis fermentation liquid (RH-SFL) was used as the carbon source for nitrogen removal, the nitrogen removal effect was close to sodium acetate and the nitrogen removal rate (NRR) was up to 98.5 +/- 0.5%. According to the NOX--N removal curve, the reaction rate of RH-SFL was significantly higher than before re-hydrolysis (nRH-SFL) (0.486 > 0.38). This technology can significantly improve the effective organic quality in the alkaline fermentation liquid of WAS, which can be more easily utilized by denitrifying bacteria. This provides a feasible and effective solution for nitrogen removal in municipal wastewater with insufficient carbon.
Keyword :
Re-hydrolysis Re-hydrolysis Carbon source Carbon source Immobilized fillers Immobilized fillers Denitrification Denitrification Waste activated sludge Waste activated sludge
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GB/T 7714 | Liu, Xuyan , Yang, Hong , Chang, Jiang et al. Re-hydrolysis characteristics of alkaline fermentation liquid from waste activated sludge: Feasibility as a carbon source for nitrogen removal [J]. | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION , 2022 , 165 : 230-240 . |
MLA | Liu, Xuyan et al. "Re-hydrolysis characteristics of alkaline fermentation liquid from waste activated sludge: Feasibility as a carbon source for nitrogen removal" . | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION 165 (2022) : 230-240 . |
APA | Liu, Xuyan , Yang, Hong , Chang, Jiang , Bai, Yongsheng , Shi, Luyuan , Su, Bojun et al. Re-hydrolysis characteristics of alkaline fermentation liquid from waste activated sludge: Feasibility as a carbon source for nitrogen removal . | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION , 2022 , 165 , 230-240 . |
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Abstract :
The copper mine drainage (CMD) formed during copper mining and smelting has the characteristics of being low pH, with a high sulfate concentration and large amounts of metal ions such as Fe, Cu, and Mn. In order to recover copper-dominated many resources from CMD, realize effective control of environmental pollution and avoid the loss of precious resources. The combined process of Fe pretreatment recovery, Cu treatment recovery, and S treatment recovery was established based on H2O2 oxidation, sulfate-reducing bacteria (SRB) reduction, and sulfur-oxidizing bacteria oxidation. A pilot-scale study was carried out with a CMD inlet flow rate of 12 m3/d. Results show that the average Cu2+ removal rate was 99.66%, and the recovered dry matter precipitated (mainly was CuS) copper content was 35.6%. In addition, the average total Fe, Mn and Mg removal rate was 99.46%, 99.10% and 84.08%. And the S2-oxidation rate was above 90% (mainly converted to sulfur). 27.52% Fe precipitated dry matter and 42.9% S, 32.2% Mg, and 13.8% Mn precipitated dry matter were also recovered as by-products. Through high-throughput analysis of the functional and structural changes in the SRB community, showed that indigenous SRB from the copper mine exhibit good adaptability to CMD conditions, with the enrichment and acclimation of SRBs achieving a high level of expression of bioactive. The bottleneck of SRB applied to CMD treatment was solved. Finally, the economic feasibility analysis shows that the combined process treatment CMD can save 58.6% of the cost compared with traditional lime neutralization method, and avoid the sludge treatment cost of 29.03 million USD per year. The above provides engineering practical reference for CMD treatment and resource recovery.
Keyword :
Resources recovery Resources recovery Sulfate-reducing bacteria Sulfate-reducing bacteria Economic feasibility analysis Economic feasibility analysis Copper mine drainage Copper mine drainage Combined process Combined process
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GB/T 7714 | Hu, Xin , Yang, Hong , Wu, Fan et al. Recovery of copper-dominated resources from copper mine drainage by chemical oxidation and sulfur biocycling: A pilot-scale study [J]. | JOURNAL OF CLEANER PRODUCTION , 2022 , 378 . |
MLA | Hu, Xin et al. "Recovery of copper-dominated resources from copper mine drainage by chemical oxidation and sulfur biocycling: A pilot-scale study" . | JOURNAL OF CLEANER PRODUCTION 378 (2022) . |
APA | Hu, Xin , Yang, Hong , Wu, Fan , Fang, Xiaoyue , Tan, Keyan . Recovery of copper-dominated resources from copper mine drainage by chemical oxidation and sulfur biocycling: A pilot-scale study . | JOURNAL OF CLEANER PRODUCTION , 2022 , 378 . |
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Abstract :
The nitrogen removal characteristics, physicochemical properties, and microbial community composition of four different anaerobic ammonium oxidation (anammox) sludge morphologies were investigated. The morphologies considered in this study, namely suspended sludge (Rs), biofilm (Rm), granular sludge (Rg), and encapsulated biomass (Re), were prepared from floc sludge. The results show that Re exhibited the maximum anammox activity, followed by Rg, Rm, and Rs. Additionally, the anammox contribution rate was higher in Rg and Re. The higher extracellular polymer content in Rg promoted sludge accumulation, and tryptophan was observed in Rm and Rg, which was replaced by humic acids in Rs. Re showed the largest specific surface area, hydrophobicity and strength, and its good structure ensured enrichment of anammox bacteria (AnAOB). In terms of the microbial community, the functional bacterium Candidatus Kuenenia accounted for the highest proportion in Rm (39.27%), but the presence of both anaerobic and aerobic regions led to increased community complexity with more nitrifying bacteria. In contrast, Rg and Re had a more specific microbial community. In addition, denitrifying bacteria tended to grow in Rs, while nitrifying bacteria were retained in Rm. The AnAOB were more likely to be enriched in sludge aggregates (both Rm and Rg) and carriers (Re). Through correlation analysis, the potential relationship involving bacterial flora evolution of each sample was clarified. Finally, the structural models of different morphologies of sludge were proposed. This study deepens the understanding of various anammox sludge morphologies as well as provides useful information for the cultivation of AnAOB and further application of anammox.
Keyword :
Anammox Anammox Granular sludge Granular sludge Flora structure Flora structure Immobilized filler Immobilized filler Biofilm Biofilm Sludge morphology Sludge morphology
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GB/T 7714 | Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community [J]. | CHEMOSPHERE , 2022 , 288 . |
MLA | Wang, XiaoTong et al. "Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community" . | CHEMOSPHERE 288 (2022) . |
APA | Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community . | CHEMOSPHERE , 2022 , 288 . |
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Abstract :
In this study, the effects of different storage conditions, such as temperature, storage time and biomass form, on the properties of anaerobic ammonia oxidation (anammox) were investigated along with the identification of the process mechanism. The results showed that the influence of storage time on anammox properties was stronger than that of storage temperature and biomass form. Also, the anammox recovery activity at 15 degrees C was better than that at 4 degrees C, and the anammox recovery activity of immobilized filler was better than that of anammox granular sludge (AnGS). Although cryogenic storage severely damaged anammox activity, lower loss of extracellular polymeric substances maintained the AnGS structure. The maximum recovery of specific anammox activity at 15 degrees C for the immobilized filler was observed to be 109%. In addition, intermittent substrate supplementation weakened the adverse effect of long-term storage on anammox activity, and was conducive to maintaining stable flora composition and promoting regeneration of anammox bacteria (AnAOB). High-throughput sequencing analysis showed that starvation resulted in increased community diversity, and the functional bacteria Candidatus Brocadia was observed to be more tolerant to starvation than Candidatus Kuenenia. Finally, principal component analysis was used to explain the complex relationship between process performance and preservation conditions. Based on the results of this work, it is recommended to preserve AnAOB in the form of immobilized filler at 15 degrees C and supplement substrate intermittently during long term storage. This study provides an economical and robust strategy for the short-term and long-term preservation of AnAOB. (c) 2021 Elsevier B.V. All rights reserved.
Keyword :
Preservation conditions Preservation conditions Active recovery Active recovery Immobilized filler Immobilized filler Flora structure Flora structure Anammox bacteria Anammox bacteria
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GB/T 7714 | Wang, XiaoTong , Yang, Hong , Geng, LiangHan et al. Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 816 . |
MLA | Wang, XiaoTong et al. "Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form" . | SCIENCE OF THE TOTAL ENVIRONMENT 816 (2022) . |
APA | Wang, XiaoTong , Yang, Hong , Geng, LiangHan , Liu, XuYan . Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 816 . |
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Abstract :
The immobilized carrier was prepared with complete anaerobic ammonia oxidation granular sludge (AnGS) and crushed AnGS, respectively. We evaluated the effects of granular form and continuous temperature changes on nitrogen removal by immobilized anaerobic ammonium oxidation (anammox) filler. The results showed that the rate of nitrogen removal of crushed and encapsulated AnGS was 20% higher than that of direct encapsulated AnGS. However, the latter had higher thresholds of tolerance to Fe2+ and Cu2+. In addition, the immobilization reduced the activation energy of anammox. Above 12.5 degrees C, the immobilized filler was efficient at removing nitrogen removal through the dual adjustment of temperature-hydraulic retention time. From 12.5 degrees C to 23 degrees C, the temperature had a greater influence on the nitrogen removal effect than the HRT. In contrast, HRT had a dominant influence from 23 degrees C to 32 degrees C. Anammox activity was severely inhibited below 12.5 degrees C. High-throughput sequencing analysis showed that the community structure migrated with the changes in temperature. The anammox functional bacteria Candidatus Kuenenia (18.31-39.73%) were the dominant genus at medium and high temperatures, and it was replaced by Chryseobacterium (24.19%) at 8.5 degrees C. In addition, an RDA analysis showed that Candidatus Brocadia was more adaptable to low temperatures than Candidatus Kuenenia. In addition, Bellilinea was more sensitive to temperature than Candidatus Kuenenia. Thus, the temperature could be appropriately lowered to avoid overbreeding. The results of this study optimized the operation of an anammox immobilized system and promote its further application. (C) 2021 Elsevier B.V. All rights reserved.
Keyword :
Anammox Anammox Temperature Temperature AnGS form AnGS form Metal ions Metal ions Microflora structure Microflora structure Immobilized filler Immobilized filler
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GB/T 7714 | Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
MLA | Wang, XiaoTong et al. "Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis" . | SCIENCE OF THE TOTAL ENVIRONMENT 803 (2022) . |
APA | Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
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Abstract :
Four anaerobic ammonium oxidation (anammox) immobilized filler reactors (R1: 33 degrees C-normal, R2: seasonal temperature-normal, R3: seasonal temperature-feast, R4: seasonal temperature-starvation) were established to study the response of anammox immobilized fillers to seasonal temperature changes and different operating modes. The results showed that the anammox immobilized filler could better adapt to the seasonal temperature drop and maintain the activity potential by adjusting the hydraulic retention time (HRT). During the temperature rise phase, R2 activity increased rapidly with the highest nitrogen removal rate reaching 1.26 kgN center dot(m3 center dot d)-1, which was equivalent to control sample R1 (1.33 kgN center dot(m3 center dot d)-1). However, feasting and famine conditions severely impaired anammox performance and changed stoichiometric ratios; feasting, in particular, significantly lowered the nitrogen removal potential of R3. The specific anammox activity of R2, R3 and R4 was 92.2%, 52.6% and 67.9%, respectively, that of R1, respectively, where the accumulation of functional bacteria was the reason for the higher activity of R2. Degradation kinetics and NO2--N inhibition curves showed that R3 was less sensitive to high concentrations of NH4+-N, while R4 responded earlier to low concentrations of NH4+-N, and the reduction of IC50 at low temperature was the reason for the inhibition of R3 activity. Furthermore, seasonal temperature fluctuations had little effect on the microbial community structure but had a considerable impact on bacteria abundance. The anammox functional bacteria Candidatus Kuenenia was found to be the dominant genus in R1-R4; however, the relative abundance of most bacteria, including anammox bacteria, decreased in R3, while the proportion of fermentation bacteria and denitrifying bacteria increased in R4. These findings highlight the necessity of rational regulation of HRT for the adaptation of anammox immobilized fillers to seasonal temperature changes, which could enhance our understanding of the synergistic effect of seasonal temperature changes and different operating modes on nitrogen removal.
Keyword :
Anammox Anammox Immobilized fillers Immobilized fillers Microbial community Microbial community Feast-famine condition Feast-famine condition Seasonal temperature change Seasonal temperature change
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GB/T 7714 | Wang, XiaoTong , Yang, Hong . Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 829 . |
MLA | Wang, XiaoTong et al. "Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis" . | SCIENCE OF THE TOTAL ENVIRONMENT 829 (2022) . |
APA | Wang, XiaoTong , Yang, Hong . Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 829 . |
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Abstract :
The treatment performance of anaerobic ammonia oxidation (anammox) immobilized filler on different proportions of domestic wastewater was evaluated. The results showed that, in comparison to synthetic wastewater, 50% domestic wastewater promoted the anammox reaction of immobilized filler, while 100% domestic wastewater had no significant effect on the anammox activity of immobilized filler but the total nitrogen removal efficiency (TNRE) was improved through enhanced denitrification. The TNRE of the immobilized filler was 82.5%, which was significantly higher than that of AnGS (69.7%), and its average anammox contribution rate was more than 90%. This was because the encapsulated anammox biomass could better maintain competitive advantages and coordinate the symbiotic relationship with denitrifying bacteria. Moreover, lower NH4+-N concentration resulted in greater influence of C/N ratio on anammox performance than COD concentration, while the opposite was true at high NH4+-N concentration. This study verified that anammox immobilized filler is effective for mainstream applications.
Keyword :
Microbial composition Microbial composition Domestic wastewater Domestic wastewater Anammox Anammox Immobilized filler Immobilized filler C/N ratio C/N ratio
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GB/T 7714 | Wang, Xiao Tong , Yang, Hong , Zhou, YaKun et al. Performance and mechanism analysis of gel immobilized anammox bacteria in treating different proportions of domestic wastewater: a valid alternative to granular sludge [J]. | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
MLA | Wang, Xiao Tong et al. "Performance and mechanism analysis of gel immobilized anammox bacteria in treating different proportions of domestic wastewater: a valid alternative to granular sludge" . | BIORESOURCE TECHNOLOGY 347 (2022) . |
APA | Wang, Xiao Tong , Yang, Hong , Zhou, YaKun , Liu, XuYan . Performance and mechanism analysis of gel immobilized anammox bacteria in treating different proportions of domestic wastewater: a valid alternative to granular sludge . | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
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Abstract :
This study focused on treating municipal wastewater using a novel two-stage partial-nitritation/anammox (PN/ A) process by gel immobilization. The first stage employed a PN immobilized carrier reactor, in which the nitrite accumulation rate was 90.7-95.1 % and the COD removal rate was 33.2 +/- 2.9 %. The second stage comprised an anammox-based sequencing batch reactor (SBR) fed with the effluent from the first stage. Two second stage SBRs were tested to compare their treatment effect, one contained anammox granules (AG-SBR) and one contained anammox immobilized carriers (AC-SBR). The AC-SBR exhibited better nitrogen removal performance than the AG-SBR during the PN/A process, with the nitrogen removal rate of AC-SBR being 83.4 +/- 1.5 %. The gel immobilization technology effectively reduced the adverse effects of dissolved oxygen and organic matter in the PN reactor effluent on the anammox system. Aerobic denitrification was observed in the AG-SBR. In the PN immobilized carrier, Nitrospira was the dominant nitrite-oxidizing bacteria (NOB) with a relative abundance of 0.10 %, indicating that the growth of NOB was effectively inhibited in municipal wastewater. Candidatus Kue-nenia was the dominant genus of anammox bacteria, exhibiting a relative abundance of 1.50 % and 19.26 % in the AG-SBR and AC-SBR, respectively. Thus, the PN/A process by gel immobilization is an effective treatment for municipal wastewater.
Keyword :
Gel immobilization Gel immobilization Municipal wastewater Municipal wastewater Two -stage PN Two -stage PN Anammox immobilized carrier Anammox immobilized carrier A process A process Anammox granules Anammox granules
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GB/T 7714 | Wang, Jiawei , Zhang, Si , Li, Jun et al. Municipal wastewater treatment via the two-stage partial nitrification-anammox (PN/A) process with gel immobilization [J]. | JOURNAL OF WATER PROCESS ENGINEERING , 2022 , 50 . |
MLA | Wang, Jiawei et al. "Municipal wastewater treatment via the two-stage partial nitrification-anammox (PN/A) process with gel immobilization" . | JOURNAL OF WATER PROCESS ENGINEERING 50 (2022) . |
APA | Wang, Jiawei , Zhang, Si , Li, Jun , Yang, Hong . Municipal wastewater treatment via the two-stage partial nitrification-anammox (PN/A) process with gel immobilization . | JOURNAL OF WATER PROCESS ENGINEERING , 2022 , 50 . |
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