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Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration SCIE
期刊论文 | 2024 , 12 (5) | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
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Abstract :

A gel filler with diatomaceous earth, activated carbon, and CaCO3 compounded with polyvinyl alcohol was prepared and optimized. Rapid start-up and efficient and stable operation of partial nitrification (PN) was achieved by immobilization of conventional activated sludge. The results showed that the proper material proportion synergistically improved the performance and oxygen mass transfer rate of the gel. The continuous flow reactor was operated for 10d to successfully start PN, and the maximum ammonia oxidation rate of this filler was 58.30 mg center dot(L center dot h)- 1 during the stable operation, and the nitrite accumulation rate was maintained above 90 %. However, due to the greater mass transfer resistance of unoptimized fillers, more dissolved oxygen waste and nitrogen loss were caused, resulting in delayed expression of biological activity. Based on the kinetic characterization of ammonia-oxidizing bacteria (AOB) within the filler and high-throughput sequencing, it was demonstrated that the rapid start-up and efficient and stable operation of the PN system was due to the highly oxygenophilic and community-dominant position of AOB within this filler. This study provides new options and insights at the level of immobilization technology for the realization and efficient and stable operation of PN.

Keyword :

Immobilization materials Immobilization materials Partial nitrification Partial nitrification Nitrogen removal Nitrogen removal Start-up Start-up Oxygen mass transfer Oxygen mass transfer

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GB/T 7714 Zhang, Xinming , Yang, Hong . Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration [J]. | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2024 , 12 (5) .
MLA Zhang, Xinming 等. "Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration" . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING 12 . 5 (2024) .
APA Zhang, Xinming , Yang, Hong . Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2024 , 12 (5) .
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Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes SCIE
期刊论文 | 2023 , 370 | BIORESOURCE TECHNOLOGY
WoS CC Cited Count: 6
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Abstract :

A two-stage partial nitritation/anammox process based on microbial encapsulation (PN/A-E) was established. The nitrogen removal characteristics of PN/A-E under high and low ammonia nitrogen and after reactivation following a long-term shutdown were comprehensively investigated and compared with anammox granular sludge (AnGS). The stable PN process did not depend on high ammonia nitrogen, and the nitrite accumulation rate reached 95.2 +/- 0.7 %. The overall nitrogen removal rate of encapsulated anammox bacteria was twice that of the AnGS, and it was more tolerant to external interference. Moreover, PN/A-E showed good reactivation performance, and the total nitrogen in the effluent was 10.0 +/- 1.4 mg center dot L-1 when the final hydraulic retention time was 2.18 h. The immobilized fillers support an increase in ammonia-oxidizing bacteria under restricted conditions and were more conducive to the dominance of functional bacteria and the stability of microbial community under low ammonia nitrogen. This study provides a positive method to achieve a reliable PN/A.

Keyword :

Microbial community structure Microbial community structure Anammox Anammox Nitrogen removal Nitrogen removal Partial nitritation Partial nitritation Microbial immobilization Microbial immobilization

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GB/T 7714 Wang, XiaoTong , Yang, Hong , Wang, JiaWei . Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes [J]. | BIORESOURCE TECHNOLOGY , 2023 , 370 .
MLA Wang, XiaoTong 等. "Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes" . | BIORESOURCE TECHNOLOGY 370 (2023) .
APA Wang, XiaoTong , Yang, Hong , Wang, JiaWei . Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes . | BIORESOURCE TECHNOLOGY , 2023 , 370 .
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一种高积累率短程硝化活性污泥的培养方法 incoPat
专利 | 2022-03-23 | CN202210297622.X
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一种高积累率短程硝化活性污泥的培养方法,属于水处理领域。采用污水处理厂的活性污泥为种源,保持了原有短程硝化(AOB)菌群的结构特征,采用静置24h的FA抑制启动方法,帮助AOB菌群建立优势地位,并在高溶解氧(DO)的运行条件下,利用高FNA抑制了亚硝酸盐氧化菌(NOB)的生长,维持高积累率,再通过规律排泥,进而在保持系统高氨氧化速率的前提下,规律性排泥淘汰NOB,使AOB快速富集,实现高积累率短程硝化活性污泥的培养。

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GB/T 7714 杨宏 , 王泊含 . 一种高积累率短程硝化活性污泥的培养方法 : CN202210297622.X[P]. | 2022-03-23 .
MLA 杨宏 等. "一种高积累率短程硝化活性污泥的培养方法" : CN202210297622.X. | 2022-03-23 .
APA 杨宏 , 王泊含 . 一种高积累率短程硝化活性污泥的培养方法 : CN202210297622.X. | 2022-03-23 .
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Municipal wastewater treatment via the two-stage partial nitrification-anammox (PN/A) process with gel immobilization SCIE
期刊论文 | 2022 , 50 | JOURNAL OF WATER PROCESS ENGINEERING
WoS CC Cited Count: 9
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Abstract :

This study focused on treating municipal wastewater using a novel two-stage partial-nitritation/anammox (PN/ A) process by gel immobilization. The first stage employed a PN immobilized carrier reactor, in which the nitrite accumulation rate was 90.7-95.1 % and the COD removal rate was 33.2 +/- 2.9 %. The second stage comprised an anammox-based sequencing batch reactor (SBR) fed with the effluent from the first stage. Two second stage SBRs were tested to compare their treatment effect, one contained anammox granules (AG-SBR) and one contained anammox immobilized carriers (AC-SBR). The AC-SBR exhibited better nitrogen removal performance than the AG-SBR during the PN/A process, with the nitrogen removal rate of AC-SBR being 83.4 +/- 1.5 %. The gel immobilization technology effectively reduced the adverse effects of dissolved oxygen and organic matter in the PN reactor effluent on the anammox system. Aerobic denitrification was observed in the AG-SBR. In the PN immobilized carrier, Nitrospira was the dominant nitrite-oxidizing bacteria (NOB) with a relative abundance of 0.10 %, indicating that the growth of NOB was effectively inhibited in municipal wastewater. Candidatus Kue-nenia was the dominant genus of anammox bacteria, exhibiting a relative abundance of 1.50 % and 19.26 % in the AG-SBR and AC-SBR, respectively. Thus, the PN/A process by gel immobilization is an effective treatment for municipal wastewater.

Keyword :

Gel immobilization Gel immobilization Municipal wastewater Municipal wastewater Two -stage PN Two -stage PN Anammox immobilized carrier Anammox immobilized carrier A process A process Anammox granules Anammox granules

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GB/T 7714 Wang, Jiawei , Zhang, Si , Li, Jun et al. Municipal wastewater treatment via the two-stage partial nitrification-anammox (PN/A) process with gel immobilization [J]. | JOURNAL OF WATER PROCESS ENGINEERING , 2022 , 50 .
MLA Wang, Jiawei et al. "Municipal wastewater treatment via the two-stage partial nitrification-anammox (PN/A) process with gel immobilization" . | JOURNAL OF WATER PROCESS ENGINEERING 50 (2022) .
APA Wang, Jiawei , Zhang, Si , Li, Jun , Yang, Hong . Municipal wastewater treatment via the two-stage partial nitrification-anammox (PN/A) process with gel immobilization . | JOURNAL OF WATER PROCESS ENGINEERING , 2022 , 50 .
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Recovery of copper-dominated resources from copper mine drainage by chemical oxidation and sulfur biocycling: A pilot-scale study SCIE
期刊论文 | 2022 , 378 | JOURNAL OF CLEANER PRODUCTION
WoS CC Cited Count: 5
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Abstract :

The copper mine drainage (CMD) formed during copper mining and smelting has the characteristics of being low pH, with a high sulfate concentration and large amounts of metal ions such as Fe, Cu, and Mn. In order to recover copper-dominated many resources from CMD, realize effective control of environmental pollution and avoid the loss of precious resources. The combined process of Fe pretreatment recovery, Cu treatment recovery, and S treatment recovery was established based on H2O2 oxidation, sulfate-reducing bacteria (SRB) reduction, and sulfur-oxidizing bacteria oxidation. A pilot-scale study was carried out with a CMD inlet flow rate of 12 m3/d. Results show that the average Cu2+ removal rate was 99.66%, and the recovered dry matter precipitated (mainly was CuS) copper content was 35.6%. In addition, the average total Fe, Mn and Mg removal rate was 99.46%, 99.10% and 84.08%. And the S2-oxidation rate was above 90% (mainly converted to sulfur). 27.52% Fe precipitated dry matter and 42.9% S, 32.2% Mg, and 13.8% Mn precipitated dry matter were also recovered as by-products. Through high-throughput analysis of the functional and structural changes in the SRB community, showed that indigenous SRB from the copper mine exhibit good adaptability to CMD conditions, with the enrichment and acclimation of SRBs achieving a high level of expression of bioactive. The bottleneck of SRB applied to CMD treatment was solved. Finally, the economic feasibility analysis shows that the combined process treatment CMD can save 58.6% of the cost compared with traditional lime neutralization method, and avoid the sludge treatment cost of 29.03 million USD per year. The above provides engineering practical reference for CMD treatment and resource recovery.

Keyword :

Resources recovery Resources recovery Sulfate-reducing bacteria Sulfate-reducing bacteria Economic feasibility analysis Economic feasibility analysis Copper mine drainage Copper mine drainage Combined process Combined process

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GB/T 7714 Hu, Xin , Yang, Hong , Wu, Fan et al. Recovery of copper-dominated resources from copper mine drainage by chemical oxidation and sulfur biocycling: A pilot-scale study [J]. | JOURNAL OF CLEANER PRODUCTION , 2022 , 378 .
MLA Hu, Xin et al. "Recovery of copper-dominated resources from copper mine drainage by chemical oxidation and sulfur biocycling: A pilot-scale study" . | JOURNAL OF CLEANER PRODUCTION 378 (2022) .
APA Hu, Xin , Yang, Hong , Wu, Fan , Fang, Xiaoyue , Tan, Keyan . Recovery of copper-dominated resources from copper mine drainage by chemical oxidation and sulfur biocycling: A pilot-scale study . | JOURNAL OF CLEANER PRODUCTION , 2022 , 378 .
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基于包埋填料的短程硝化反硝化工艺的脱氮性能优化
期刊论文 | 2022 , 16 (06) , 1979-1990 | 环境工程学报
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为优化短程硝化反硝化工艺对模拟城市污水的脱氮处理性能,通过包埋固定化技术分别制得短程硝化、反硝化填料,形成连续流脱氮工艺。结果表明,短程硝化填料活性恢复后的氨氧化速率(AOR)可达39.83mg·(L·h)-1,亚硝酸盐积累率(NAR)稳定在96.60%。采用响应曲面法考察了短程硝化过程中的DO、HRT和填充率等因素对氨氮去除率(ARR)和NAR的影响,并建立了二次回归模型,通过模型预测的最佳运行工况为:HRT为3.48 h,DO为3.64 mg·L~(-1),填充率为20%。此时,后置反硝化包埋填料,当平均C/N为2.82时,总氮出水平均质量浓度为2.29 mg·L~(-1),去除率稳定在9...

Keyword :

反硝化 反硝化 短程硝化 短程硝化 包埋填料 包埋填料 响应曲面法 响应曲面法

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GB/T 7714 方小月 , 杨宏 , 刘旭妍 et al. 基于包埋填料的短程硝化反硝化工艺的脱氮性能优化 [J]. | 环境工程学报 , 2022 , 16 (06) : 1979-1990 .
MLA 方小月 et al. "基于包埋填料的短程硝化反硝化工艺的脱氮性能优化" . | 环境工程学报 16 . 06 (2022) : 1979-1990 .
APA 方小月 , 杨宏 , 刘旭妍 , 王晓曈 , 胡鑫 . 基于包埋填料的短程硝化反硝化工艺的脱氮性能优化 . | 环境工程学报 , 2022 , 16 (06) , 1979-1990 .
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Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form SCIE
期刊论文 | 2022 , 816 | SCIENCE OF THE TOTAL ENVIRONMENT
WoS CC Cited Count: 7
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Abstract :

In this study, the effects of different storage conditions, such as temperature, storage time and biomass form, on the properties of anaerobic ammonia oxidation (anammox) were investigated along with the identification of the process mechanism. The results showed that the influence of storage time on anammox properties was stronger than that of storage temperature and biomass form. Also, the anammox recovery activity at 15 degrees C was better than that at 4 degrees C, and the anammox recovery activity of immobilized filler was better than that of anammox granular sludge (AnGS). Although cryogenic storage severely damaged anammox activity, lower loss of extracellular polymeric substances maintained the AnGS structure. The maximum recovery of specific anammox activity at 15 degrees C for the immobilized filler was observed to be 109%. In addition, intermittent substrate supplementation weakened the adverse effect of long-term storage on anammox activity, and was conducive to maintaining stable flora composition and promoting regeneration of anammox bacteria (AnAOB). High-throughput sequencing analysis showed that starvation resulted in increased community diversity, and the functional bacteria Candidatus Brocadia was observed to be more tolerant to starvation than Candidatus Kuenenia. Finally, principal component analysis was used to explain the complex relationship between process performance and preservation conditions. Based on the results of this work, it is recommended to preserve AnAOB in the form of immobilized filler at 15 degrees C and supplement substrate intermittently during long term storage. This study provides an economical and robust strategy for the short-term and long-term preservation of AnAOB. (c) 2021 Elsevier B.V. All rights reserved.

Keyword :

Preservation conditions Preservation conditions Active recovery Active recovery Immobilized filler Immobilized filler Flora structure Flora structure Anammox bacteria Anammox bacteria

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GB/T 7714 Wang, XiaoTong , Yang, Hong , Geng, LiangHan et al. Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 816 .
MLA Wang, XiaoTong et al. "Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form" . | SCIENCE OF THE TOTAL ENVIRONMENT 816 (2022) .
APA Wang, XiaoTong , Yang, Hong , Geng, LiangHan , Liu, XuYan . Analyzing the effect of storage conditions on anammox recovery performance from the perspectives of time, temperature and biomass form . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 816 .
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Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis SCIE
期刊论文 | 2022 , 803 | SCIENCE OF THE TOTAL ENVIRONMENT
WoS CC Cited Count: 20
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Abstract :

The immobilized carrier was prepared with complete anaerobic ammonia oxidation granular sludge (AnGS) and crushed AnGS, respectively. We evaluated the effects of granular form and continuous temperature changes on nitrogen removal by immobilized anaerobic ammonium oxidation (anammox) filler. The results showed that the rate of nitrogen removal of crushed and encapsulated AnGS was 20% higher than that of direct encapsulated AnGS. However, the latter had higher thresholds of tolerance to Fe2+ and Cu2+. In addition, the immobilization reduced the activation energy of anammox. Above 12.5 degrees C, the immobilized filler was efficient at removing nitrogen removal through the dual adjustment of temperature-hydraulic retention time. From 12.5 degrees C to 23 degrees C, the temperature had a greater influence on the nitrogen removal effect than the HRT. In contrast, HRT had a dominant influence from 23 degrees C to 32 degrees C. Anammox activity was severely inhibited below 12.5 degrees C. High-throughput sequencing analysis showed that the community structure migrated with the changes in temperature. The anammox functional bacteria Candidatus Kuenenia (18.31-39.73%) were the dominant genus at medium and high temperatures, and it was replaced by Chryseobacterium (24.19%) at 8.5 degrees C. In addition, an RDA analysis showed that Candidatus Brocadia was more adaptable to low temperatures than Candidatus Kuenenia. In addition, Bellilinea was more sensitive to temperature than Candidatus Kuenenia. Thus, the temperature could be appropriately lowered to avoid overbreeding. The results of this study optimized the operation of an anammox immobilized system and promote its further application. (C) 2021 Elsevier B.V. All rights reserved.

Keyword :

Anammox Anammox Temperature Temperature AnGS form AnGS form Metal ions Metal ions Microflora structure Microflora structure Immobilized filler Immobilized filler

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GB/T 7714 Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 .
MLA Wang, XiaoTong et al. "Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis" . | SCIENCE OF THE TOTAL ENVIRONMENT 803 (2022) .
APA Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 .
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Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis SCIE
期刊论文 | 2022 , 829 | SCIENCE OF THE TOTAL ENVIRONMENT
WoS CC Cited Count: 6
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Four anaerobic ammonium oxidation (anammox) immobilized filler reactors (R1: 33 degrees C-normal, R2: seasonal temperature-normal, R3: seasonal temperature-feast, R4: seasonal temperature-starvation) were established to study the response of anammox immobilized fillers to seasonal temperature changes and different operating modes. The results showed that the anammox immobilized filler could better adapt to the seasonal temperature drop and maintain the activity potential by adjusting the hydraulic retention time (HRT). During the temperature rise phase, R2 activity increased rapidly with the highest nitrogen removal rate reaching 1.26 kgN center dot(m3 center dot d)-1, which was equivalent to control sample R1 (1.33 kgN center dot(m3 center dot d)-1). However, feasting and famine conditions severely impaired anammox performance and changed stoichiometric ratios; feasting, in particular, significantly lowered the nitrogen removal potential of R3. The specific anammox activity of R2, R3 and R4 was 92.2%, 52.6% and 67.9%, respectively, that of R1, respectively, where the accumulation of functional bacteria was the reason for the higher activity of R2. Degradation kinetics and NO2--N inhibition curves showed that R3 was less sensitive to high concentrations of NH4+-N, while R4 responded earlier to low concentrations of NH4+-N, and the reduction of IC50 at low temperature was the reason for the inhibition of R3 activity. Furthermore, seasonal temperature fluctuations had little effect on the microbial community structure but had a considerable impact on bacteria abundance. The anammox functional bacteria Candidatus Kuenenia was found to be the dominant genus in R1-R4; however, the relative abundance of most bacteria, including anammox bacteria, decreased in R3, while the proportion of fermentation bacteria and denitrifying bacteria increased in R4. These findings highlight the necessity of rational regulation of HRT for the adaptation of anammox immobilized fillers to seasonal temperature changes, which could enhance our understanding of the synergistic effect of seasonal temperature changes and different operating modes on nitrogen removal.

Keyword :

Anammox Anammox Immobilized fillers Immobilized fillers Microbial community Microbial community Feast-famine condition Feast-famine condition Seasonal temperature change Seasonal temperature change

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GB/T 7714 Wang, XiaoTong , Yang, Hong . Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 829 .
MLA Wang, XiaoTong et al. "Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis" . | SCIENCE OF THE TOTAL ENVIRONMENT 829 (2022) .
APA Wang, XiaoTong , Yang, Hong . Nitrogen removal performance of anammox immobilized fillers in response to seasonal temperature variations and different operating modes: Substrate utilization and microbial community analysis . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 829 .
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Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community SCIE
期刊论文 | 2022 , 288 | CHEMOSPHERE
WoS CC Cited Count: 16
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The nitrogen removal characteristics, physicochemical properties, and microbial community composition of four different anaerobic ammonium oxidation (anammox) sludge morphologies were investigated. The morphologies considered in this study, namely suspended sludge (Rs), biofilm (Rm), granular sludge (Rg), and encapsulated biomass (Re), were prepared from floc sludge. The results show that Re exhibited the maximum anammox activity, followed by Rg, Rm, and Rs. Additionally, the anammox contribution rate was higher in Rg and Re. The higher extracellular polymer content in Rg promoted sludge accumulation, and tryptophan was observed in Rm and Rg, which was replaced by humic acids in Rs. Re showed the largest specific surface area, hydrophobicity and strength, and its good structure ensured enrichment of anammox bacteria (AnAOB). In terms of the microbial community, the functional bacterium Candidatus Kuenenia accounted for the highest proportion in Rm (39.27%), but the presence of both anaerobic and aerobic regions led to increased community complexity with more nitrifying bacteria. In contrast, Rg and Re had a more specific microbial community. In addition, denitrifying bacteria tended to grow in Rs, while nitrifying bacteria were retained in Rm. The AnAOB were more likely to be enriched in sludge aggregates (both Rm and Rg) and carriers (Re). Through correlation analysis, the potential relationship involving bacterial flora evolution of each sample was clarified. Finally, the structural models of different morphologies of sludge were proposed. This study deepens the understanding of various anammox sludge morphologies as well as provides useful information for the cultivation of AnAOB and further application of anammox.

Keyword :

Anammox Anammox Granular sludge Granular sludge Flora structure Flora structure Immobilized filler Immobilized filler Biofilm Biofilm Sludge morphology Sludge morphology

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GB/T 7714 Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community [J]. | CHEMOSPHERE , 2022 , 288 .
MLA Wang, XiaoTong et al. "Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community" . | CHEMOSPHERE 288 (2022) .
APA Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effects of sludge morphology on the anammox process: Analysis from the perspectives of performance, structure, and microbial community . | CHEMOSPHERE , 2022 , 288 .
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