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学者姓名：罗云敬
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Abstract ：
In this study, morin magnetic molecularly imprinted polymers (Morin-MMIPs) were synthesized based on magnetic nanoparticles and surface molecularly imprinted technology with superparamagnetism and extraction selectivity. The polymers allowed the separating of morin from complex matrices in the presence of an external magnetic field with no need for centrifugation or filtration. The microstructure of the polymers was characterized by scanning electron microscopy and transmission electron microscopy. Meanwhile, the functional group and magnetic properties of the polymers were characterized using Fourier transform infrared spectroscopy (FT-IR) and magnetic vibration meter (VSM). The maximum adsorption capacity of MMIPs was 3.24 mg/g, which was 2.55 times higher than that of MNIPs (1.27 mg/g). Morin was quantified by HPLC-DAD, which showed good linearity in the concentration range of 0.05-60 mu g/ml with the correlation coefficient R-2 = 0.9993. The limit of detection (LOD) was 0.08 mu g/ml, and the spiked recoveries were 87.5-106.8%. The calculation of the adsorption isotherm and kinetic model revealed the adsorption mechanisms, and the adsorption process was consistent with the Langmuir adsorption isotherm and pseudo-secondary kinetic models. Likewise, the material has been successfully used to extract and separate morin from food samples. The method reported in this paper has the advantages of fast adsorption speed, high selectivity, and environmental friendliness. It provided a reliable method for the separation and detection of morin or other natural products.
Keyword ：
magnetic molecularly imprinted polymers magnetic molecularly imprinted polymers molecular imprinting technology molecular imprinting technology selective extraction selective extraction morin morin
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| GB/T 7714 | Zhang, Wei , Luo, Yunjing , Xie, Ziqi et al. Extraction and detection of morin from Sanghuangporus lonicericola by magnetic molecularly imprinted polymers coupled with HPLC analysis [J]. | JOURNAL OF FOOD SCIENCE , 2022 , 87 (4) : 1575-1585 . |
| MLA | Zhang, Wei et al. "Extraction and detection of morin from Sanghuangporus lonicericola by magnetic molecularly imprinted polymers coupled with HPLC analysis" . | JOURNAL OF FOOD SCIENCE 87 . 4 (2022) : 1575-1585 . |
| APA | Zhang, Wei , Luo, Yunjing , Xie, Ziqi , Kong, Chenchen , Na, Zhen . Extraction and detection of morin from Sanghuangporus lonicericola by magnetic molecularly imprinted polymers coupled with HPLC analysis . | JOURNAL OF FOOD SCIENCE , 2022 , 87 (4) , 1575-1585 . |
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Abstract ：
Based on gold nanoclusters (AuNCs) with good optical stability, biocompatibility and simple and non-toxic preparation method, this paper developed a highly selective, highly sensitive and visualized uric acid (UA) sensor. We synthesized BSA-AuNCs using bovine serum albumin (BSA) as a template. Under the catalysis of urate oxidase, UA produces stoichiometric hydrogen peroxide (H2O2), which causes the fluorescence of AuNCs to be quenched. In addition, we found that BSA-AuNCs mimic the peroxidase activity in this system, catalysing the substrate's oxidation 3,3',5,5'-tetramethylbenzidine (TMB) to ox-TMB by H2O2. At this time, the emission spectrum of BSA-AuNCs overlaps the absorption spectrum of ox-TMB, and a kind of fluorescence resonance energy transfer (FRET) occurs. BSA-AuNCs acts as a donor to transfer the excitation energy to the acceptor ox-TMB, which makes ox-TMB produce fluorescence. At the same time, the fluorescence intensity of BSA-AuNCs is significantly lower than when it exists alone, which significantly improves the sensitivity of UA detection. Under optimum conditions, it is found that the UA concentration has an excellent linear relationship between the quenching degree of 2 similar to 100 mu mol . L-1, the linear equation is (F-0 - F)/F-0=0. 005 85c(UA) + 0. 103 64, the linear correlation coefficient is 0. 995 4, and the detection limit is 0. 26 mu mol . L-1, which is far below the minimum limit of normal human UA levels (90 mu mol . L-1). Meanwhile, the recovery of UA in blood samples was studied, and the recovery rate was 97. 3% to 104. 7%, indicating that this method is effective in clinical blood samples. The detection has tremendous application potential, and provides an excellent theoretical basis and methodological guidance for further clinical analysis.
Keyword ：
Hydrogen peroxide Hydrogen peroxide Fluorescence resonance energy transfer Fluorescence resonance energy transfer Gold nanoclusters Gold nanoclusters Uric acid Uric acid
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| GB/T 7714 | Cong Jian-han , Luo Yun-jing , Qi Xiao-hua et al. Sensitive Detection of Uric Acid Based on BSA Gold Nanoclusters by Fluorescence Energy Resonance Transfer [J]. | SPECTROSCOPY AND SPECTRAL ANALYSIS , 2022 , 42 (2) : 483-489 . |
| MLA | Cong Jian-han et al. "Sensitive Detection of Uric Acid Based on BSA Gold Nanoclusters by Fluorescence Energy Resonance Transfer" . | SPECTROSCOPY AND SPECTRAL ANALYSIS 42 . 2 (2022) : 483-489 . |
| APA | Cong Jian-han , Luo Yun-jing , Qi Xiao-hua , Zou Ming-qiang , Kong Chen-chen . Sensitive Detection of Uric Acid Based on BSA Gold Nanoclusters by Fluorescence Energy Resonance Transfer . | SPECTROSCOPY AND SPECTRAL ANALYSIS , 2022 , 42 (2) , 483-489 . |
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Abstract ：
Gold nanoclusters have good biocompatibility and can be easily modified to improve their luminescence properties. In this study, we prepared a new type of dual-emitting gold nanoclusters (d-Au NCs) for discriminative detection of phenylalanine and Fe3+ with high selectivity and sensitivity. The fluorescence sensor which was synthesized without any further assembly or conjugation shows dual-emissions at 430 nm and 600 nm under a single excitation at 350 nm. Phenylalanine can turn on the red emission of the probe, while Fe3+ can turn on its yellow emission and turn off the red emission. By detecting a variety of amino acids and metal ions, d-Au NCs showed good selectivity to phenylalanine and Fe3+. Finally, this method was applied to determine phenylalanine and Fe3+ in lake water, human urine and milk, which has certain application prospects in the field of biology and environment.
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| GB/T 7714 | Kong, Chenchen , Luo, Yunjing , Zhang, Wei et al. A ratio fluorescence method based on dual emissive gold nanoclusters for detection of biomolecules and metal ions [J]. | RSC ADVANCES , 2022 , 12 (19) : 12060-12067 . |
| MLA | Kong, Chenchen et al. "A ratio fluorescence method based on dual emissive gold nanoclusters for detection of biomolecules and metal ions" . | RSC ADVANCES 12 . 19 (2022) : 12060-12067 . |
| APA | Kong, Chenchen , Luo, Yunjing , Zhang, Wei , Lin, Taifeng , Na, Zhen , Liu, Xuemei et al. A ratio fluorescence method based on dual emissive gold nanoclusters for detection of biomolecules and metal ions . | RSC ADVANCES , 2022 , 12 (19) , 12060-12067 . |
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Abstract ：
This study aimed to assess the ameliorative effect and mechanisms of sulforaphane (SFN) on cadmium (Cd)induced hepatotoxicity. Four in vitro free radical scavenging tests, HepG2 cell viability analysis, and cadmium chloride (CdCl2) indeced liver injured mice mode were employed. SFN scavenged the free radicals in vitro, and mitigated the oxidative damages in HepG2 cells incubated with SFN + CdCl2. In mice models, SFN pretreatment dose-dependently remarkably improved redox homeostasis, and attenuated the expressions of the key liver inflammatory factors (TNF-alpha, IL-6, IL-1 beta) by CdCl2. The pathological examinations were consistent with the above results. Moreover, both mRNA and protein expression of hepatic nuclear factor-erythroid 2-related factor 2 and hemeoxygenase-1 increased, while nuclear factor-kappa B reduced in Cd + SFN co-treated groups. Taken together, this study firstly demonstrated the anti-oxidant and anti-inflammatory effects of SFN against Cd induced liver damages, which associated with the modulation of intrinsic Nrf2/ARE and NF-kappa B pathway.
Keyword ：
Inflammation Inflammation Hepatotoxicity Hepatotoxicity Sulforaphen Sulforaphen Cadmium Cadmium Oxidative stress Oxidative stress
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| GB/T 7714 | He, Qingfeng , Luo, Yunjing , Xie, Ziqi . Sulforaphane ameliorates cadmium induced hepatotoxicity through the up-regulation of /Nrf2/ARE pathway and the inactivation of NF-kappa B [J]. | JOURNAL OF FUNCTIONAL FOODS , 2021 , 77 . |
| MLA | He, Qingfeng et al. "Sulforaphane ameliorates cadmium induced hepatotoxicity through the up-regulation of /Nrf2/ARE pathway and the inactivation of NF-kappa B" . | JOURNAL OF FUNCTIONAL FOODS 77 (2021) . |
| APA | He, Qingfeng , Luo, Yunjing , Xie, Ziqi . Sulforaphane ameliorates cadmium induced hepatotoxicity through the up-regulation of /Nrf2/ARE pathway and the inactivation of NF-kappa B . | JOURNAL OF FUNCTIONAL FOODS , 2021 , 77 . |
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Abstract ：
In this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed utilizing a surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs was characterized by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated that the diameter of the Gen-MMIPs was approximately 500 nm. Via analysis with a vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs was determined to be 24.79 emu g(-1). Fourier transform infrared (FT-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiments suggested that the genistein adsorption capability of Gen-MMIPs was 5.81 mg g(-1), and adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction (dSPE) adsorbents combined with HPLC were used to selectively separate genistein in soy sauce samples, and the recoveries ranged from 85.7 to 88.5% with relative standard deviations (RSDs) less than 5%, which proved that this method can be used for the detection of genistein residues in real samples.
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| GB/T 7714 | Xie, Ziqi , Luo, Yunjing , Na, Zhen et al. Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products [J]. | SCIENTIFIC REPORTS , 2021 , 11 (1) . |
| MLA | Xie, Ziqi et al. "Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products" . | SCIENTIFIC REPORTS 11 . 1 (2021) . |
| APA | Xie, Ziqi , Luo, Yunjing , Na, Zhen , Zhang, Wei , Zong, Yufei . Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products . | SCIENTIFIC REPORTS , 2021 , 11 (1) . |
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Abstract ：
Glycoprotein 96 (gp96) is a highly conserved and ubiquitous glycoprotein that belongs to the heat shock protein 90 (HSP90) family. It comprises 4 domains: N-terminal domain (NTD), middle domain (MD), C-terminal domain (CTD) and charged linker region (CR). Each domain performs a specific function. NTD containing the nucleotide binding site interacts with and hydrolyzes ATP. MD is involved in client protein recognition, and is the site of constitutive dimerization. The ATP hydrolytic activity of NTD requires cooperative action of CR and MD. The schematic representation of gp96 topology is shown in the main text. An increased expression of gp96 has been reported in multiple cancers. Its upregulation in tumors is closely correlated with poor prognosis and decreased overall survival of patients, indicating that gp96 serves as a potential diagnostic and prognostic biomarker. As a chaperone protein gp96 directs the folding and/or assembly of secreted and membrane proteins. It has a limited client protein profile that is involved in key processes linked with the hallmarks of cancer. Previous studies have shown that cellular gp96 physically interacts with and directs the folding and assembly of several client proteins, including insulin-like growth factors (IGF), integrins, epidermal growth factor receptor-2 (HER2) and Wnt co-receptor LRP6, which are involved in the regulation of cell multiplication, normal tissue differentiation, cancer progression and metastasis. It has been found that gp96 was only expressed on cell surface of malignant tumor but not benign tissues. Cell membrane gp96 is closely associated with cancer cell proliferation, invasion, and metastasis. We further demonstrated that gp96 on cell membrane interacts with HER2, urokinase-type plasminogen activator-receptor (uPAR) or ER-alpha 36. Targeting gp96 by siRNA or a monoclonal antibody for gp96 led to decreased cell growth and invasion, increased apoptosis in vitro, and suppression of tumor growth in vivo, validating cell membrane gp96 as a therapeutic target. At present, the selective small-molecule inhibitors (NECA and PU-WS13), a gp96-specific monoclonal antibody (W9mAb), and an inhibitory gp96-targeted polypeptide (p37) are under development and their potential applications in the tumor targeting therapy are highlighted in this review.
Keyword ：
heat shock protein gp96 heat shock protein gp96 molecular chaperone molecular chaperone targeted therapy targeted therapy cancer cancer cell membrane gp96 cell membrane gp96
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| GB/T 7714 | Qian Li-Yuan , Li Chang-Fei , Luo Yun-Jing et al. Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression [J]. | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS , 2021 , 48 (9) : 993-1005 . |
| MLA | Qian Li-Yuan et al. "Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression" . | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 48 . 9 (2021) : 993-1005 . |
| APA | Qian Li-Yuan , Li Chang-Fei , Luo Yun-Jing , Meng Song-Dong . Research Progress of The Role of Heat Shock Protein gp96 in Cancer Development and Progression . | PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS , 2021 , 48 (9) , 993-1005 . |
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Abstract ：
肝细胞癌(Hepatocellular carcinoma,HCC)是致死率第3的恶性肿瘤,也是全球第5大常见癌症。肝癌在临床上的治疗手段非常有限,患者的总生存率也很低。因此,肝癌的早期诊断和治疗对于患者总生存率有着重要的影响。甲胎蛋白(Alpha-fetoprotein,AFP)是最早发现也是目前应用最广泛的肝癌标志物之一。目前,多项研究表明,作为一个特异性的癌基因,AFP在肝癌的发生、发展、诊断和治疗中有巨大的研究价值。文中简述了AFP在肝癌发生发展中的分子调控机制以及在肝癌细胞逃避免疫监视中的作用,着重阐述AFP作为重要的肝癌靶标分子在肝癌的临床诊断和治疗研究中的应用。
Keyword ：
甲胎蛋白 甲胎蛋白 肝癌治疗 肝癌治疗 肝癌 肝癌 临床诊断 临床诊断 血清学标志物 血清学标志物
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| GB/T 7714 | 钱丽媛 , 李长菲 , 罗云敬 et al. 甲胎蛋白在肝癌的诊断和治疗中的研究进展 [J]. | 生物工程学报 , 2021 , 37 (09) : 3042-3060 . |
| MLA | 钱丽媛 et al. "甲胎蛋白在肝癌的诊断和治疗中的研究进展" . | 生物工程学报 37 . 09 (2021) : 3042-3060 . |
| APA | 钱丽媛 , 李长菲 , 罗云敬 , 孟颂东 . 甲胎蛋白在肝癌的诊断和治疗中的研究进展 . | 生物工程学报 , 2021 , 37 (09) , 3042-3060 . |
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Abstract ：
热休克蛋白90B1又称为糖蛋白96 (gp96). gp96属于热休克蛋白90家族,是一种高度保守且普遍存在的糖蛋白.作为一种内质网蛋白,gp96在维持内质网稳态、内质网应激、钙稳态等方面起着重要的调控作用,这些调控网络在肿瘤的发生发展过程中起着重要的作用. gp96作为分子伴侣在稳定和激活客户蛋白等方面有众多报道,其中包括HER2、整合素和Toll样受体等多个客户蛋白.大量研究表明,gp96在肝癌、乳腺癌、胃癌等不同类型的肿瘤中高表达,并在肿瘤的生长、侵袭和转移等方面起着重要的作用.本文从gp96的基本结构和功能及其在肿瘤发生发展中的作用等方面进行综述,并着重阐述细胞膜gp96相关的研究进展...
Keyword ：
热休克蛋白gp96 热休克蛋白gp96 靶向治疗 靶向治疗 肿瘤 肿瘤 分子伴侣 分子伴侣 胞膜gp96 胞膜gp96
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| GB/T 7714 | 钱丽媛 , 李长菲 , 罗云敬 et al. 热休克蛋白gp96在恶性肿瘤中的研究进展 [J]. | 生物化学与生物物理进展 , 2021 , 48 (09) : 993-1005 . |
| MLA | 钱丽媛 et al. "热休克蛋白gp96在恶性肿瘤中的研究进展" . | 生物化学与生物物理进展 48 . 09 (2021) : 993-1005 . |
| APA | 钱丽媛 , 李长菲 , 罗云敬 , 孟颂东 . 热休克蛋白gp96在恶性肿瘤中的研究进展 . | 生物化学与生物物理进展 , 2021 , 48 (09) , 993-1005 . |
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Abstract ：
An liquid chromatography-tandem mass spectrometry method coupled with a stable isotope dilution assay was established for the simultaneous detection of 17 mycotoxins and their derivatives (aflatoxins B1 , B2 , G1 , G2 , M1 , and M2 ; fumonisins B1 and B2 ; ochratoxin A; zearalenone; zearalanone; α-zearalanol; α-zearalenol; T-2 toxin; deoxynivalenol; deepoxy-deoxynivalenol; and sterigmatocystin) in milk and dairy products. The mycotoxins were extracted with acidified acetonitrile and the lipids were removed using a Captiva EMR-lipid column. The average recoveries of the target compounds from samples spiked at three different concentrations were 67-102%, and the relative standard deviations of the peak areas were less than 10%. Limits of quantification (S/N = 10) of 0.004-1.25 μg/kg were achieved, which are significantly lower than the maximum levels allowed in various countries and regions for each regulated mycotoxin. Milk and yogurt products from local markets and e-commercial platforms were analyzed using the optimized method. The screening showed that aflatoxin M1 , deoxynivalenol, fumonisins B1 and B2 , and zearalenone could be found in milk and yogurt products, especially those products also containing grains or jujube ingredients, indicating that there is a risk of mycotoxins in dairy products.
Keyword ：
multimycotoxins multimycotoxins isotope dilution isotope dilution risk assessment risk assessment dairy dairy
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| GB/T 7714 | Yang Shuai , Luo Yunjing , Mu Lei et al. Risk screening of mycotoxins and their derivatives in dairy products using a stable isotope dilution assay and LC-MS/MS. [J]. | Journal of separation science , 2021 , 44 (4) : 782-792 . |
| MLA | Yang Shuai et al. "Risk screening of mycotoxins and their derivatives in dairy products using a stable isotope dilution assay and LC-MS/MS." . | Journal of separation science 44 . 4 (2021) : 782-792 . |
| APA | Yang Shuai , Luo Yunjing , Mu Lei , Yang Youyou , Yang Yongtan . Risk screening of mycotoxins and their derivatives in dairy products using a stable isotope dilution assay and LC-MS/MS. . | Journal of separation science , 2021 , 44 (4) , 782-792 . |
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Abstract ：
基于分步液液提取以及脂质净化柱净化的样品前处理方式,建立同时测定食用植物油中28种真菌毒素的高效液相色谱-串联质谱的测定方法。28种真菌毒素的定量限范围0.07～1.25μg/kg,三水平加标回收率范围60%～120%,峰面积相对标准偏差小于12.5%,均满足国家标准GB/T 27404-2008《实验室质量控制规范食品理化检测》中实验室质量控制规范以及真菌毒素限量标准的要求。应用本方法对市售食用植物油样品进行真菌毒素的风险监测,结果显示:黄曲霉毒素、玉米赤霉烯酮及其衍生物、杂色曲霉素、T-2毒素有不同程度的检出。其中,花生油中主要的真菌毒素种类为黄曲霉毒素、杂色曲霉素以及玉米赤霉烯酮;菜籽油...
Keyword ：
食用油 食用油 脂质净化 脂质净化 风险监测 风险监测 真菌毒素 真菌毒素
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| GB/T 7714 | 杨帅 , 杨永坛 , 穆蕾 et al. 液相色谱-串联质谱同时测定食用植物油中的多种真菌毒素 [J]. | 中国食品学报 , 2021 , 21 (05) : 309-316 . |
| MLA | 杨帅 et al. "液相色谱-串联质谱同时测定食用植物油中的多种真菌毒素" . | 中国食品学报 21 . 05 (2021) : 309-316 . |
| APA | 杨帅 , 杨永坛 , 穆蕾 , 杨霞 , 杨悠悠 , 罗云敬 . 液相色谱-串联质谱同时测定食用植物油中的多种真菌毒素 . | 中国食品学报 , 2021 , 21 (05) , 309-316 . |
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