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学者姓名：盛望
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Abstract ：
BackgroundCombinatorial immunotherapy strategies for enhancing the responsiveness of immune system have shown great promise for cancer therapy. Engineered nanoformulation incorporated toll-like receptor (TLR) 9 agonist CpG ODN has shown more positive results in suppressing tumor growth and can significantly enhance other immunotherapy activity with combinatorial effects due to the innate and adaptive immunostimulatory effects of CpG.ResultsIn the present work, protamine sulfate (PS) and carboxymethyl beta-glucan (CMG) were used as nanomaterials to form nanoparticles through a self-assembly approach for CpG ODN encapsulation to generate CpG ODN-loaded nano-adjuvant (CNPs), which was subsequently mixed with the mixture of mouse melanoma-derived antigens of tumor cell lysates (TCL) and neoantigens to develop vaccine for anti-tumor immunotherapy. The obtained results showed that CNPs was able to effectively deliver CpG ODN into murine bone marrow-derived dendritic cells (DC) in vitro, and remarkably stimulate the maturation of DC cells with proinflammatory cytokine secretion. In addition, in vivo analysis showed that CNPs enhanced anti-tumor activity of PD1 antibody and CNPs-adjuvanted vaccine based on the mixture antigens of melanoma TCL and melanoma-specific neoantigen could not only induce anti-melanoma cellular immune responses, but also elicit melanoma specific humoral immune responses, which significantly inhibited xenograft tumor growth. Furthermore, CD16 CAR-T cells were generated by expressing CD16-CAR in CD3(+)CD8(+) murine T cells.ConclusionOur results eventually showed that anti-melanoma antibodies induced by CNPs-adjuvanted TCL vaccines were able to collaborate with CD16-CAR-T cells to generate an enhanced targeted anti-tumor effects through ADCC (antibody dependent cell cytotoxicity) approach. CD16 CAR-T cells has thus a great potential to be an universal promising strategy targeting on solid tumor synergistic immunotherapy via co-operation with TCL-based vaccine.
Keyword ：
CpG ODN CpG ODN Neoantigen Neoantigen CD16 CD16 Vaccine Vaccine Immunotherapy Immunotherapy
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| GB/T 7714 | Zhang, Xiaofei , Hu, Qin , He, Xuesong et al. CD16 CAR-T cells enhance antitumor activity of CpG ODN-loaded nanoparticle-adjuvanted tumor antigen-derived vaccinevia ADCC approach [J]. | JOURNAL OF NANOBIOTECHNOLOGY , 2023 , 21 (1) . |
| MLA | Zhang, Xiaofei et al. "CD16 CAR-T cells enhance antitumor activity of CpG ODN-loaded nanoparticle-adjuvanted tumor antigen-derived vaccinevia ADCC approach" . | JOURNAL OF NANOBIOTECHNOLOGY 21 . 1 (2023) . |
| APA | Zhang, Xiaofei , Hu, Qin , He, Xuesong , Cui, Xinyue , Liang, Zhaoyuan , Wang, Li et al. CD16 CAR-T cells enhance antitumor activity of CpG ODN-loaded nanoparticle-adjuvanted tumor antigen-derived vaccinevia ADCC approach . | JOURNAL OF NANOBIOTECHNOLOGY , 2023 , 21 (1) . |
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| GB/T 7714 | Wang, Li , Li, Jintao , Zhang, Na et al. Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy (vol 532, pg 179, 2020) [J]. | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2021 , 558 : 239-239 . |
| MLA | Wang, Li et al. "Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy (vol 532, pg 179, 2020)" . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 558 (2021) : 239-239 . |
| APA | Wang, Li , Li, Jintao , Zhang, Na , Zhang, Xiaofei , Xia, Yang , Chai, Binbin et al. Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy (vol 532, pg 179, 2020) . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2021 , 558 , 239-239 . |
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In the present study, we evaluated adjuvant potential of Poria cocos polysaccharide (PCP) on the Th1-type immune responses of C57/BL6 mice against ovalbumin (OVA). We first determined the effect of PCP on maturation of murine bone marrow derived dendritic cells (BMDCs), PCP significantly upregulated surface expression of MHCII, CD40, CD80, CD86 and enhanced production of IL-6 and IL-12p40. In addition, PCP affected receptor-mediated endocytosis, but not pinocytosis in BMDCs. Furthermore, OVA + PCP immunization induced specific cytotoxic CD8+ T cell killing of OVA (257-264) peptide pulsed cell. When mice were immunized subcutaneously in a week interval with OVA + PCP. Serum were collected for measuring OVA-specific antibody and splenocytes were harvested for analyzing CD69, IFN-γ ELISpot and cytokines production. The result indicated that OVA-specific IgG, IgG2a and IgG1 antibody levels in serum were significantly elevated by PCP compared with control. PCP increased OVA-specific IFN-γ-secreting CD8+, CD4+ T cells, promoted CD8+ T cell proliferation and up-regulated Th-1 type (IFN-γ, IL-2) cytokine production. In conclusion, data suggest that PCP enhanced cellular immune response and possess potential as a vaccine adjuvant for Th1 immune response.
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| GB/T 7714 | Dong Xiaoxiao , Li Boye , Yu Boyang et al. Poria cocos polysaccharide induced Th1-type immune responses to ovalbumin in mice. [J]. | PloS one , 2021 , 16 (1) : e0245207 . |
| MLA | Dong Xiaoxiao et al. "Poria cocos polysaccharide induced Th1-type immune responses to ovalbumin in mice." . | PloS one 16 . 1 (2021) : e0245207 . |
| APA | Dong Xiaoxiao , Li Boye , Yu Boyang , Chen Tian , Hu Qin , Peng Bo et al. Poria cocos polysaccharide induced Th1-type immune responses to ovalbumin in mice. . | PloS one , 2021 , 16 (1) , e0245207 . |
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Uniting combinational strategies has been confirmed to be a robust choice for high-performance cancer treatment due to their abilities to overcome tumor heterogeneity and complexity. However, the development of a simple, effective, and multifunctional theranostics nanoplatform still remains a challenge. In this study, we integrated multicomponent hyaluronic acid (HA), protamine (PS), nanodiamonds (NDs), curcumin (Cur), and IR780 into a single nanoplatform (denoted as HPNDIC) based on the combination of hydrophobic and electrostatic noncovalent interactions for dual-modal fluorescence/photoacoustic imaging guided ternary collaborative Cur/photothermal/photodynamic combination therapy of triple-negative breast cancer (TNBC). A two-step coordination assembly strategy was utilized to realize this purpose. In the first step, PS was utilized to modify the NDs clusters to form positively charged PS@NDs (PND) and the simultaneous encapsulation of the natural small-molecule drug Cur and the photosensitive small-molecule IR780 (PNDIC). Second, HA was adsorbed onto the outer surface of the PNDIC through charge complexation for endowing a tumor-targeting ability (HPNDIC). The resulting HPNDIC had a uniform size, high drug-loading ability, and excellent colloidal stability. It was found that under the near-infrared irradiation condition, IR780 could be triggered to exhibit both PTT/PDT dual-pattern therapy effects, leading to an enhanced therapy efficiency of Cur both in vitro and in vivo with good biocompatibility. Due to the intrinsic imaging property of IR780, the biodistribution and accumulation behavior of HPNDIC in vivo could be monitored by dual-modal fluorescence/photoacoustic imaging. Taken together, our current work demonstrated the assembly of a NDs-based multicomponent theranostic platform for dual-modal fluorescence/photoacoustic imaging guided triple-collaborative Cur/photothermal/photodynamic against TNBC.
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| GB/T 7714 | Cui Xinyue , Deng Xiongwei , Liang Zhaoyuan et al. Multicomponent-assembled nanodiamond hybrids for targeted and imaging guided triple-negative breast cancer therapy via a ternary collaborative strategy. [J]. | Biomaterials science , 2021 , 9 (10) : 3838-3850 . |
| MLA | Cui Xinyue et al. "Multicomponent-assembled nanodiamond hybrids for targeted and imaging guided triple-negative breast cancer therapy via a ternary collaborative strategy." . | Biomaterials science 9 . 10 (2021) : 3838-3850 . |
| APA | Cui Xinyue , Deng Xiongwei , Liang Zhaoyuan , Lu Jianqing , Shao Leihou , Wang Xuan et al. Multicomponent-assembled nanodiamond hybrids for targeted and imaging guided triple-negative breast cancer therapy via a ternary collaborative strategy. . | Biomaterials science , 2021 , 9 (10) , 3838-3850 . |
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Traditional brazing quality inspection methods find it difficult to detect brazing layer defects on heteromorphic workpieces. Thus, a non-destructive testing technology based on a thermal probe is developed in this work. Scanning thermal resistance testing and analysis are carried out for two types of workpiece samples with different structures, and an evaluation calculation method is proposed to effectively characterize the brazing effect of the workpiece. By comparison with standard workpieces, qualified brazing layer products can be selected. In addition, the feasibility of this method is verified by ANSYS thermal simulations. In comparison with the x ray, it also has shown the superiority of this method. Experimental results show that this method can effectively evaluate the brazing layer quality of workpieces with heteromorphic and complex structures, and the reliability of the workpiece is further improved. Published under an exclusive license by AIP Publishing.
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| GB/T 7714 | Hu, Chaoxu , Feng, Shiwei , Zhang, Yamin et al. A new method for detecting heteromorphic workpiece brazing layer quality based on thermal probe [J]. | REVIEW OF SCIENTIFIC INSTRUMENTS , 2021 , 92 (8) . |
| MLA | Hu, Chaoxu et al. "A new method for detecting heteromorphic workpiece brazing layer quality based on thermal probe" . | REVIEW OF SCIENTIFIC INSTRUMENTS 92 . 8 (2021) . |
| APA | Hu, Chaoxu , Feng, Shiwei , Zhang, Yamin , He, Xin , Bai, Kun , Wang, Sheng et al. A new method for detecting heteromorphic workpiece brazing layer quality based on thermal probe . | REVIEW OF SCIENTIFIC INSTRUMENTS , 2021 , 92 (8) . |
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Abstract ：
Owing to its aggressive biological behavior, the lack of specific targets, and the strong therapeutic resistance of triple negative breast cancer (TNBC), current therapeutic strategies are still limited. The combination of multiple treatments has been confirmed as a promising strategy for TNBC therapy. However, the efficacy of combination therapy can be restricted due to increasing therapeutic resistance to various treatments. Herein, we constructed a nanodiamond (ND)-based nanoplatform for augmented mild-temperature photothermal/chemo combination therapy against TNBC, weakening the therapeutic resistance via autophagy inhibition enabled by the NDs. A layer-by-layer self-assembly approach was utilized to construct the ND-based nanoplatform. First, the NDs were modified with protamine sulphate (PS). Meanwhile, the photosensitizer indocyanine green (ICG) and the HSP70 small molecule inhibitor apoptozole (APZ) could be synchronously incorporated to form positively charged PS@ND (ICG + APZ). Then negatively charged hyaluronic acid (HA) was assembled onto the outer face of PS@ND (ICG + APZ) to form the NPIAs. Finally, the positively charged small molecule anti-cancer drug doxorubicin (DOX) could be adsorbed onto the surface of the NPIAs through electrostatic interactions (NPIADs). The resulting NPIADs could be triggered by NIR laser irradiation to exhibit enhanced mild-temperature photothermal therapy (PTT) effects via suppressing the expression of HSP70, and PTT combined with chemotherapy could further enhance the anti-tumor efficacy. Subsequently, the sensitivity of MDA-MB-231 cells could be significantly improved through the weakening of the thermal/drug resistance via autophagy inhibition, leading to augmented combination therapy that is efficient both in vitro and in vivo. Furthermore, the NPIADs could be used as a theranostic nanoplatform for fluorescence (FL) and photoacoustic (PA) imaging. Taken together, this study demonstrated a multifunctional ND-based nanoplatform for FL/PA imaging-guided augmented mild-temperature photothermal/chemo combination therapy via an autophagy regulation strategy against TNBC.
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| GB/T 7714 | Cui, Xinyue , Liang, Zhaoyuan , Lu, Jianqing et al. A multifunctional nanodiamond-based nanoplatform for the enhanced mild-temperature photothermal/chemo combination therapy of triple negative breast cancer via an autophagy regulation strategy [J]. | NANOSCALE , 2021 , 13 (31) : 13375-13389 . |
| MLA | Cui, Xinyue et al. "A multifunctional nanodiamond-based nanoplatform for the enhanced mild-temperature photothermal/chemo combination therapy of triple negative breast cancer via an autophagy regulation strategy" . | NANOSCALE 13 . 31 (2021) : 13375-13389 . |
| APA | Cui, Xinyue , Liang, Zhaoyuan , Lu, Jianqing , Wang, Xuan , Jia, Fan , Hu, Qin et al. A multifunctional nanodiamond-based nanoplatform for the enhanced mild-temperature photothermal/chemo combination therapy of triple negative breast cancer via an autophagy regulation strategy . | NANOSCALE , 2021 , 13 (31) , 13375-13389 . |
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Cancer vaccines targeting tumor specific neoantigens derived from nonsynonymous mutations of tumor cells have emerged as an effective approach to induce antitumor T cells responses for personalized cancer immunotherapy. Despite the enormous potential of synthetic peptides as a common modality for neoantigen vaccines, their practical efficacy was limited due to their relatively low immunogenicity. Herein, we modify neoantigen peptide (Adpgk) derived from MC-38 colon carcinoma by supplementing ten consecutive positively-charged lysines (10 K-Adpgk) to obtain cationic polypeptide. And then we made them self-assemble with toll-like receptor 9 (TLR-9) agonist CpG oligodeoxynucleotides (CpG ODN) adjuvant directly forming antigen/adjuvant integrated nanocomplexes (PCNPs) through electrostatic interaction for potent tumor immunotherapy. The optimal formed PCNPs were around 175 nm with uniform size distribution and could maintain stability in physiological saline solution. CpG ODN and 10 K-Adpgk in the formed PCNPs could be effectively uptake by dendritic cells (DCs) and stimulate the maturation of DCs as well as improving the efficiency of antigen crosspresentation efficiency in vitro. Furthermore, the PCNPs vaccine could markedly improve neoantigen and adjuvant co-delivery efficiency to lymphoid organs and activate cytotoxic T cells. In addition, vaccination with PCNPs could not only offer prophylactic to protect mice from challenged MC-38 colorectal tumors, but also achieve a better anti-tumor effect in an established colorectal tumor model, and significantly prolong the survival rate of tumor-bearing mice. Therefore, this work provided a versatile but effective method for neoantigen peptide and CpG ODN co-assembly vaccine platform for efficient colorectal cancer immunotherapy.
Keyword ：
CpG ODN CpG ODN Neoantigen Neoantigen Immunotherapy Immunotherapy Peptide Peptide Nanovaccine Nanovaccine
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| GB/T 7714 | Liang, Zhaoyuan , Cui, Xinyue , Yang, Liqun et al. Co-assembled nanocomplexes of peptide neoantigen Adpgk and Toll-like receptor 9 agonist CpG ODN for efficient colorectal cancer immunotherapy [J]. | INTERNATIONAL JOURNAL OF PHARMACEUTICS , 2021 , 608 . |
| MLA | Liang, Zhaoyuan et al. "Co-assembled nanocomplexes of peptide neoantigen Adpgk and Toll-like receptor 9 agonist CpG ODN for efficient colorectal cancer immunotherapy" . | INTERNATIONAL JOURNAL OF PHARMACEUTICS 608 (2021) . |
| APA | Liang, Zhaoyuan , Cui, Xinyue , Yang, Liqun , Hu, Qin , Li, Danyang , Zhang, Xiaofei et al. Co-assembled nanocomplexes of peptide neoantigen Adpgk and Toll-like receptor 9 agonist CpG ODN for efficient colorectal cancer immunotherapy . | INTERNATIONAL JOURNAL OF PHARMACEUTICS , 2021 , 608 . |
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Digital sensor arrays based on ring oscillators are used to monitor the temperature of Field Programmable Gate Array (FPGA) chips, but traditional sensor structures using binary frequency counters consume excessive hardware logic resources. This paper proposed a compact temperature sensor array that used low-decoding-complexity linear feedback shift registers (LFSR) as a frequency counter to efficiently count the ring oscillator frequency. We implemented an experimental system on a Spartan-6 FPGA, and the logic resource consumption of the entire system was 927 Look-Up-Tables (LUTs) with an overhead of only 10%. The single sampling time was approximately 40 μs. The proposed temperature sensor array has good temperature sensing capabilities, and the measured error is ±0.9°C(3σ). © 2021 Elsevier Ltd
Keyword ：
Temperature sensors Temperature sensors Logic gates Logic gates Shift registers Shift registers Field programmable gate arrays (FPGA) Field programmable gate arrays (FPGA) Table lookup Table lookup Computer circuits Computer circuits
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| GB/T 7714 | Wang, Sheng , Feng, Shiwei , Xiao, Yuxuan et al. Build-in compact and efficient temperature sensor array on field programmable gate array [J]. | Microelectronics Journal , 2021 , 111 . |
| MLA | Wang, Sheng et al. "Build-in compact and efficient temperature sensor array on field programmable gate array" . | Microelectronics Journal 111 (2021) . |
| APA | Wang, Sheng , Feng, Shiwei , Xiao, Yuxuan , Hu, Chaoxu , Pan, Shijie . Build-in compact and efficient temperature sensor array on field programmable gate array . | Microelectronics Journal , 2021 , 111 . |
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Abstract ：
Human papillomavirus (HPV) infection was associated with some carcinomas, especially malignant tumors in upper digestive tract, upper respiratory tract, and genitourinary system. The mechanism of the viral transformation of normal cells is still not very clear. To investigate the tumorigenesis of epithelial cells, E6/E7-induced malignant transformation model cells were used for expression profiling analysis by performing RNA expression microarray detection. Bioinformatics analysis was applied to investigate the cellular process changes along with the E6/E7 expression in SHEE cells. The differentially expressed genes were further grouped and uploaded for Search Tool for the Retrieval of Interacting Genes analysis. The protein-protein interaction results were visualized. The hub genes and their first-neighbors genes were selected, followed by gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis. The obtained results demonstrated that tumor-related biological processes began to emerge during the carcinogenesis process from 48th passage to 76th passage of SHEE cells after E6/E7 expression. Ten hub genes were identified and analyzed during the E6/E7-induced tumorigenesis. This study explores the gene expression network in the progressive transformation of immortalized esophageal epithelial cells induced by E6/E7 expression. Understanding the biological processes and hub genes that first appear during the transformation will provide some clues to the mechanism of E6/E7-induced carcinogenesis of esophageal epithelial cells.
Keyword ：
E6 E6 HPV HPV E7 E7 bioinformatics bioinformatics esophageal epithelial cells esophageal epithelial cells
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| GB/T 7714 | Xia, Yang , Li, Jintao , Li, Shuying et al. Identification of pathways and genes in the process of E6/E7-induced carcinogenesis of esophageal epithelial cells [J]. | JOURNAL OF MEDICAL VIROLOGY , 2020 , 92 (12) : 3736-3742 . |
| MLA | Xia, Yang et al. "Identification of pathways and genes in the process of E6/E7-induced carcinogenesis of esophageal epithelial cells" . | JOURNAL OF MEDICAL VIROLOGY 92 . 12 (2020) : 3736-3742 . |
| APA | Xia, Yang , Li, Jintao , Li, Shuying , Khodahemmati, Sara , Ghaffar, Maliha , Chen, Su et al. Identification of pathways and genes in the process of E6/E7-induced carcinogenesis of esophageal epithelial cells . | JOURNAL OF MEDICAL VIROLOGY , 2020 , 92 (12) , 3736-3742 . |
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Epidermal growth factor receptor (EGFR) is an important target for tumor therapy in various tumors. The current understanding of EGFR conformations on the cell surface is based on X-ray structural data, molecular dynamic simulations, and fluorescence-localization imaging. Using scanning electron microscope (SEM) and transmission electron microscope (TEM) with the resolution at sub-nanometers, we successfully recognized individual molecules of EGFRs and their assembly details on the surface of triple-negative breast cancer (TNBC) upon one-to-one labeling by Au nanoparticles. Based on our results, we have proposed the possible configurations, structural models, and conformational transitions of EGFR oligomers. Our study shows that the high-resolution electron imaging is an invaluable tool to provide direct evidence of EGFR configuration on tumor cell surfaces, and may play a pivotal role in further understanding of EGFR-associated signaling and tumor therapy. (C) 2020 Elsevier Inc. All rights reserved.
Keyword ：
TEM TEM Structural model Structural model SEM SEM EGFR EGFR Configuration Configuration Oligomerization Oligomerization
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| GB/T 7714 | Wang, Li , Li, Jintao , Zhang, Na et al. Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy [J]. | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2020 , 532 (2) : 179-184 . |
| MLA | Wang, Li et al. "Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy" . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 532 . 2 (2020) : 179-184 . |
| APA | Wang, Li , Li, Jintao , Zhang, Na , Zhang, Xiaofei , Xia, Yang , Chai, Binbin et al. Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2020 , 532 (2) , 179-184 . |
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