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学者姓名：盛望
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Abstract ：
Cyanobacterial cytochrome c6 (Cyt c6) is crucial for electron transfer between the cytochrome b6f complex and photosystem I (PSI), playing a key role in photosynthesis and enhancing adaptation to extreme environments. This study investigates the high-resolution crystal structures of Cyt c6 from Synechococcus elongatus PCC 7942 and Synechocystis PCC 6803, focusing on its dimerization mechanisms and functional implications for photosynthesis. Cyt c6 was expressed in Escherichia coli using a dual-plasmid co-expression system and characterized in both oxidized and reduced states. X-ray crystallography revealed three distinct crystal forms, with asymmetric units containing 2, 4, or 12 molecules, all of which consist of repeating dimeric structures. Structural comparisons across species indicated that dimerization predominantly occurs through hydrophobic interactions within a conserved motif around the heme crevice, despite notable variations in dimer positioning. We propose that the dimerization of Cyt c6 enhances structural stability, optimizes electron transfer kinetics, and protects the protein from oxidative damage. Furthermore, we used AlphaFold3 to predict the structure of the PSI-Cyt c6 complex, revealing specific interactions that may facilitate efficient electron transfer. These findings provide new insights into the functional role of Cyt c6 dimerization and its contribution to improving cyanobacterial photosynthetic electron transport.
Keyword ：
cytochrome c6 cytochrome c6 photosystem I photosystem I dimerization dimerization X-ray crystallography X-ray crystallography cyanobacteria cyanobacteria electron transfer electron transfer
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| GB/T 7714 | Zhang, Botao , Xu, Yuancong , Liu, Shuwen et al. A High-Resolution Crystallographic Study of Cytochrome c6: Structural Basis for Electron Transfer in Cyanobacterial Photosynthesis [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (2) . |
| MLA | Zhang, Botao et al. "A High-Resolution Crystallographic Study of Cytochrome c6: Structural Basis for Electron Transfer in Cyanobacterial Photosynthesis" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 2 (2025) . |
| APA | Zhang, Botao , Xu, Yuancong , Liu, Shuwen , Chen, Sixu , Zhao, Wencong , Li, Zhaoyang et al. A High-Resolution Crystallographic Study of Cytochrome c6: Structural Basis for Electron Transfer in Cyanobacterial Photosynthesis . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (2) . |
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Ferroptosis is a form of regulated cell death (RCD) characterized by iron-dependent lipid peroxidation. Ferroptosis is currently proposed as one of the most promising means of combating tumor resistance. Nevertheless, the problem of ferroptosis resistance in certain cancer cells has been identified. This review first, investigates the mechanisms of ferroptosis induction in cancer cells. Next, the problem of cancer cell resistance to ferroptosis, as well as the underlying mechanisms is discussed. Recently discovered ferroptosis-suppressing biomarkers have been described. The various types of nanoparticles that can induce ferroptosis are also discussed. Given the ability of nanoparticles to combine multiple agents, this review proposes nanoparticle-based ferroptosis cell death as a viable method of circumventing this resistance. This review suggests combining ferroptosis with other forms of cell death, such as apoptosis, cuproptosis and autophagy. It also suggests combining ferroptosis with immunotherapy.
Keyword ：
nanoparticles nanoparticles combinatory therapy combinatory therapy cancer therapy cancer therapy ferroptosis ferroptosis ferroptosis resistance ferroptosis resistance
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| GB/T 7714 | Adzavon, Kodzo Prosper , Zhao, Weijian , He, Xuesong et al. Ferroptosis resistance in cancer cells: nanoparticles for combination therapy as a solution [J]. | FRONTIERS IN PHARMACOLOGY , 2024 , 15 . |
| MLA | Adzavon, Kodzo Prosper et al. "Ferroptosis resistance in cancer cells: nanoparticles for combination therapy as a solution" . | FRONTIERS IN PHARMACOLOGY 15 (2024) . |
| APA | Adzavon, Kodzo Prosper , Zhao, Weijian , He, Xuesong , Sheng, Wang . Ferroptosis resistance in cancer cells: nanoparticles for combination therapy as a solution . | FRONTIERS IN PHARMACOLOGY , 2024 , 15 . |
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Abstract ：
皮肤恶性黑色素瘤是恶性黑色素瘤的一种常见类型，具有极强的转移性和侵袭性，同时具有高突变负荷、肿瘤间和肿瘤内遗传异质性以及复杂的肿瘤微环境。黑色素瘤潜在机制的深度研究对于理解肿瘤进展和对治疗的反应至关重要。本文总结了单细胞转录组测序技术在黑色素瘤研究中的应用，从构建皮肤黑色素瘤的基因表达图谱、表征肿瘤间和肿瘤内的异质性和探究肿瘤微环境等方面深度剖析其在皮肤恶性黑色素瘤中的研究进展。并且介绍了目前常用的单细胞转录组数据库及其特点。最后，本文介绍了可与单细胞转录组测序技术结合应用的空间转录组技术，作为当下的研究热点，空间转录组技术与单细胞测序技术结合应用可从时间和空间两个维度重塑肿瘤微环境，为深入了解肿瘤提供了可以继续扩展的框架。
Keyword ：
Single Cell Sequencing Single Cell Sequencing 肿瘤微环境 肿瘤微环境 Drug Resistance Drug Resistance 单细胞测序 单细胞测序 黑色素瘤 黑色素瘤 Heterogeneity Heterogeneity 异质性 异质性 耐药性 耐药性 Melanoma Melanoma Tumor Microenvironment Tumor Microenvironment
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| GB/T 7714 | 王晓丽 , 韩中杰 , 韩梦洋 et al. 单细胞转录组测序技术在皮肤恶性黑色素瘤中的研究进展 [J]. | 生物医学 , 2023 , 13 (2) : 199-210 . |
| MLA | 王晓丽 et al. "单细胞转录组测序技术在皮肤恶性黑色素瘤中的研究进展" . | 生物医学 13 . 2 (2023) : 199-210 . |
| APA | 王晓丽 , 韩中杰 , 韩梦洋 , 李雅琪 , 盛望 . 单细胞转录组测序技术在皮肤恶性黑色素瘤中的研究进展 . | 生物医学 , 2023 , 13 (2) , 199-210 . |
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Abstract ：
肝细胞癌(Hepatic Cellular Carcinoma, HCC)是全球发病率较高的癌症之一且其死亡率在全球每年持续增加约2%~3%。氧化应激(Oxidative Stress, OS)是指当生物体细胞在遭遇外界刺激时，机体细胞内部产生大量超氧化物，如单线态氧(1O2)、过氧化氢(H2O2)，超氧阴离子(O2-)等，这些物质致机体细胞内氧化与抗氧化作用失衡，最终导致细胞和组织的损伤。氧化应激被认为是恶性肿瘤的起因之一。铁死亡(Ferroptosis)是近年发现的一种铁依赖性的新型细胞程序性死亡方式。本文主要阐述了肝细胞癌中氧化应激对铁死亡的影响的相关研究进展。
Keyword ：
Ferroptosis Ferroptosis Hepatic Cellular Carcinoma Hepatic Cellular Carcinoma Active Oxygen Active Oxygen 肝细胞癌 肝细胞癌 氧化应激 氧化应激 活性氧 活性氧 铁死亡 铁死亡 Oxidative Stress Oxidative Stress
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| GB/T 7714 | 李雅琪 , 徐弘庭 , 赵维坚 et al. 肝细胞癌中的铁死亡与氧化应激 [J]. | 生物医学 , 2023 , 13 (3) : 293-302 . |
| MLA | 李雅琪 et al. "肝细胞癌中的铁死亡与氧化应激" . | 生物医学 13 . 3 (2023) : 293-302 . |
| APA | 李雅琪 , 徐弘庭 , 赵维坚 , 肖向茜 , 韩梦洋 , 王晓丽 et al. 肝细胞癌中的铁死亡与氧化应激 . | 生物医学 , 2023 , 13 (3) , 293-302 . |
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Abstract ：
BackgroundCombinatorial immunotherapy strategies for enhancing the responsiveness of immune system have shown great promise for cancer therapy. Engineered nanoformulation incorporated toll-like receptor (TLR) 9 agonist CpG ODN has shown more positive results in suppressing tumor growth and can significantly enhance other immunotherapy activity with combinatorial effects due to the innate and adaptive immunostimulatory effects of CpG.ResultsIn the present work, protamine sulfate (PS) and carboxymethyl beta-glucan (CMG) were used as nanomaterials to form nanoparticles through a self-assembly approach for CpG ODN encapsulation to generate CpG ODN-loaded nano-adjuvant (CNPs), which was subsequently mixed with the mixture of mouse melanoma-derived antigens of tumor cell lysates (TCL) and neoantigens to develop vaccine for anti-tumor immunotherapy. The obtained results showed that CNPs was able to effectively deliver CpG ODN into murine bone marrow-derived dendritic cells (DC) in vitro, and remarkably stimulate the maturation of DC cells with proinflammatory cytokine secretion. In addition, in vivo analysis showed that CNPs enhanced anti-tumor activity of PD1 antibody and CNPs-adjuvanted vaccine based on the mixture antigens of melanoma TCL and melanoma-specific neoantigen could not only induce anti-melanoma cellular immune responses, but also elicit melanoma specific humoral immune responses, which significantly inhibited xenograft tumor growth. Furthermore, CD16 CAR-T cells were generated by expressing CD16-CAR in CD3(+)CD8(+) murine T cells.ConclusionOur results eventually showed that anti-melanoma antibodies induced by CNPs-adjuvanted TCL vaccines were able to collaborate with CD16-CAR-T cells to generate an enhanced targeted anti-tumor effects through ADCC (antibody dependent cell cytotoxicity) approach. CD16 CAR-T cells has thus a great potential to be an universal promising strategy targeting on solid tumor synergistic immunotherapy via co-operation with TCL-based vaccine.
Keyword ：
CpG ODN CpG ODN Neoantigen Neoantigen CD16 CD16 Vaccine Vaccine Immunotherapy Immunotherapy
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| GB/T 7714 | Zhang, Xiaofei , Hu, Qin , He, Xuesong et al. CD16 CAR-T cells enhance antitumor activity of CpG ODN-loaded nanoparticle-adjuvanted tumor antigen-derived vaccinevia ADCC approach [J]. | JOURNAL OF NANOBIOTECHNOLOGY , 2023 , 21 (1) . |
| MLA | Zhang, Xiaofei et al. "CD16 CAR-T cells enhance antitumor activity of CpG ODN-loaded nanoparticle-adjuvanted tumor antigen-derived vaccinevia ADCC approach" . | JOURNAL OF NANOBIOTECHNOLOGY 21 . 1 (2023) . |
| APA | Zhang, Xiaofei , Hu, Qin , He, Xuesong , Cui, Xinyue , Liang, Zhaoyuan , Wang, Li et al. CD16 CAR-T cells enhance antitumor activity of CpG ODN-loaded nanoparticle-adjuvanted tumor antigen-derived vaccinevia ADCC approach . | JOURNAL OF NANOBIOTECHNOLOGY , 2023 , 21 (1) . |
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| GB/T 7714 | Wang, Li , Li, Jintao , Zhang, Na et al. Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy (vol 532, pg 179, 2020) [J]. | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2021 , 558 : 239-239 . |
| MLA | Wang, Li et al. "Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy (vol 532, pg 179, 2020)" . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 558 (2021) : 239-239 . |
| APA | Wang, Li , Li, Jintao , Zhang, Na , Zhang, Xiaofei , Xia, Yang , Chai, Binbin et al. Investigations of EGFR configurations on tumor cell surface by high-resolution electron microscopy (vol 532, pg 179, 2020) . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2021 , 558 , 239-239 . |
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Abstract ：
Traditional brazing quality inspection methods find it difficult to detect brazing layer defects on heteromorphic workpieces. Thus, a non-destructive testing technology based on a thermal probe is developed in this work. Scanning thermal resistance testing and analysis are carried out for two types of workpiece samples with different structures, and an evaluation calculation method is proposed to effectively characterize the brazing effect of the workpiece. By comparison with standard workpieces, qualified brazing layer products can be selected. In addition, the feasibility of this method is verified by ANSYS thermal simulations. In comparison with the x ray, it also has shown the superiority of this method. Experimental results show that this method can effectively evaluate the brazing layer quality of workpieces with heteromorphic and complex structures, and the reliability of the workpiece is further improved. Published under an exclusive license by AIP Publishing.
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| GB/T 7714 | Hu, Chaoxu , Feng, Shiwei , Zhang, Yamin et al. A new method for detecting heteromorphic workpiece brazing layer quality based on thermal probe [J]. | REVIEW OF SCIENTIFIC INSTRUMENTS , 2021 , 92 (8) . |
| MLA | Hu, Chaoxu et al. "A new method for detecting heteromorphic workpiece brazing layer quality based on thermal probe" . | REVIEW OF SCIENTIFIC INSTRUMENTS 92 . 8 (2021) . |
| APA | Hu, Chaoxu , Feng, Shiwei , Zhang, Yamin , He, Xin , Bai, Kun , Wang, Sheng et al. A new method for detecting heteromorphic workpiece brazing layer quality based on thermal probe . | REVIEW OF SCIENTIFIC INSTRUMENTS , 2021 , 92 (8) . |
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Abstract ：
Owing to its aggressive biological behavior, the lack of specific targets, and the strong therapeutic resistance of triple negative breast cancer (TNBC), current therapeutic strategies are still limited. The combination of multiple treatments has been confirmed as a promising strategy for TNBC therapy. However, the efficacy of combination therapy can be restricted due to increasing therapeutic resistance to various treatments. Herein, we constructed a nanodiamond (ND)-based nanoplatform for augmented mild-temperature photothermal/chemo combination therapy against TNBC, weakening the therapeutic resistance via autophagy inhibition enabled by the NDs. A layer-by-layer self-assembly approach was utilized to construct the ND-based nanoplatform. First, the NDs were modified with protamine sulphate (PS). Meanwhile, the photosensitizer indocyanine green (ICG) and the HSP70 small molecule inhibitor apoptozole (APZ) could be synchronously incorporated to form positively charged PS@ND (ICG + APZ). Then negatively charged hyaluronic acid (HA) was assembled onto the outer face of PS@ND (ICG + APZ) to form the NPIAs. Finally, the positively charged small molecule anti-cancer drug doxorubicin (DOX) could be adsorbed onto the surface of the NPIAs through electrostatic interactions (NPIADs). The resulting NPIADs could be triggered by NIR laser irradiation to exhibit enhanced mild-temperature photothermal therapy (PTT) effects via suppressing the expression of HSP70, and PTT combined with chemotherapy could further enhance the anti-tumor efficacy. Subsequently, the sensitivity of MDA-MB-231 cells could be significantly improved through the weakening of the thermal/drug resistance via autophagy inhibition, leading to augmented combination therapy that is efficient both in vitro and in vivo. Furthermore, the NPIADs could be used as a theranostic nanoplatform for fluorescence (FL) and photoacoustic (PA) imaging. Taken together, this study demonstrated a multifunctional ND-based nanoplatform for FL/PA imaging-guided augmented mild-temperature photothermal/chemo combination therapy via an autophagy regulation strategy against TNBC.
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| GB/T 7714 | Cui, Xinyue , Liang, Zhaoyuan , Lu, Jianqing et al. A multifunctional nanodiamond-based nanoplatform for the enhanced mild-temperature photothermal/chemo combination therapy of triple negative breast cancer via an autophagy regulation strategy [J]. | NANOSCALE , 2021 , 13 (31) : 13375-13389 . |
| MLA | Cui, Xinyue et al. "A multifunctional nanodiamond-based nanoplatform for the enhanced mild-temperature photothermal/chemo combination therapy of triple negative breast cancer via an autophagy regulation strategy" . | NANOSCALE 13 . 31 (2021) : 13375-13389 . |
| APA | Cui, Xinyue , Liang, Zhaoyuan , Lu, Jianqing , Wang, Xuan , Jia, Fan , Hu, Qin et al. A multifunctional nanodiamond-based nanoplatform for the enhanced mild-temperature photothermal/chemo combination therapy of triple negative breast cancer via an autophagy regulation strategy . | NANOSCALE , 2021 , 13 (31) , 13375-13389 . |
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Abstract ：
Cancer vaccines targeting tumor specific neoantigens derived from nonsynonymous mutations of tumor cells have emerged as an effective approach to induce antitumor T cells responses for personalized cancer immunotherapy. Despite the enormous potential of synthetic peptides as a common modality for neoantigen vaccines, their practical efficacy was limited due to their relatively low immunogenicity. Herein, we modify neoantigen peptide (Adpgk) derived from MC-38 colon carcinoma by supplementing ten consecutive positively-charged lysines (10 K-Adpgk) to obtain cationic polypeptide. And then we made them self-assemble with toll-like receptor 9 (TLR-9) agonist CpG oligodeoxynucleotides (CpG ODN) adjuvant directly forming antigen/adjuvant integrated nanocomplexes (PCNPs) through electrostatic interaction for potent tumor immunotherapy. The optimal formed PCNPs were around 175 nm with uniform size distribution and could maintain stability in physiological saline solution. CpG ODN and 10 K-Adpgk in the formed PCNPs could be effectively uptake by dendritic cells (DCs) and stimulate the maturation of DCs as well as improving the efficiency of antigen crosspresentation efficiency in vitro. Furthermore, the PCNPs vaccine could markedly improve neoantigen and adjuvant co-delivery efficiency to lymphoid organs and activate cytotoxic T cells. In addition, vaccination with PCNPs could not only offer prophylactic to protect mice from challenged MC-38 colorectal tumors, but also achieve a better anti-tumor effect in an established colorectal tumor model, and significantly prolong the survival rate of tumor-bearing mice. Therefore, this work provided a versatile but effective method for neoantigen peptide and CpG ODN co-assembly vaccine platform for efficient colorectal cancer immunotherapy.
Keyword ：
CpG ODN CpG ODN Neoantigen Neoantigen Immunotherapy Immunotherapy Peptide Peptide Nanovaccine Nanovaccine
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| GB/T 7714 | Liang, Zhaoyuan , Cui, Xinyue , Yang, Liqun et al. Co-assembled nanocomplexes of peptide neoantigen Adpgk and Toll-like receptor 9 agonist CpG ODN for efficient colorectal cancer immunotherapy [J]. | INTERNATIONAL JOURNAL OF PHARMACEUTICS , 2021 , 608 . |
| MLA | Liang, Zhaoyuan et al. "Co-assembled nanocomplexes of peptide neoantigen Adpgk and Toll-like receptor 9 agonist CpG ODN for efficient colorectal cancer immunotherapy" . | INTERNATIONAL JOURNAL OF PHARMACEUTICS 608 (2021) . |
| APA | Liang, Zhaoyuan , Cui, Xinyue , Yang, Liqun , Hu, Qin , Li, Danyang , Zhang, Xiaofei et al. Co-assembled nanocomplexes of peptide neoantigen Adpgk and Toll-like receptor 9 agonist CpG ODN for efficient colorectal cancer immunotherapy . | INTERNATIONAL JOURNAL OF PHARMACEUTICS , 2021 , 608 . |
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Abstract ：
在微流控技术加工的网络图案化芯片上培养大鼠原代海马神经元细胞,从而探究体外神经元网络的生物学功能.目的:在培养过程中,我们设计的网络化图案来限定海马神经元的体外的粘附,生长及形成具有功能性的神经网络,在此基础上研究网络图案化的微流控芯片3D微通道中单个海马神经元的生物学功能.方法:利用膜片钳技术探测微通道中不同区域单个海马神经元的膜突触后电位,从而验证单个海马神经元可以与其他神经元细胞形成有效的突触连接,从而保证单一神经元具有基本的生物学活性;继而运用Image J软件进行神经突起结构的定量分析,并通过软件分析输出突起长度数据,并进行SPSS统计分析得到3D微通道培养单个海马神经元细胞平均突起长度.结果:3D微通道不同区域随机探测,均可探测到单个海马神经元的膜突触后电位;同时神经突起结构的定量分析反映了实际的神经元结构分布趋势,且统计分析结果显示,单个海马神经元平均突起长度为68.3μm.结论:基于微流控芯片技术的3D微通道可以使神经元细胞按照图案化的路径生长从而形成特定神经网络,而且其中培养的海马神经元均具有成熟生物学功能,可以形成有效的突触连接,也为单个海马神经元功能的探究提供了思路.
Keyword ：
海马神经元 海马神经元 微流控芯片 微流控芯片 神经元突起 神经元突起 3D微通道 3D微通道 膜突触后电位 膜突触后电位
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| GB/T 7714 | 孔宪敏 , 田姗姗 , 陈涛 et al. 基于微流控芯片的3D微通道中单个海马神经元功能的探究 [J]. | 科技通报 , 2020 , 36 (4) : 40-44,71 . |
| MLA | 孔宪敏 et al. "基于微流控芯片的3D微通道中单个海马神经元功能的探究" . | 科技通报 36 . 4 (2020) : 40-44,71 . |
| APA | 孔宪敏 , 田姗姗 , 陈涛 , 盛望 . 基于微流控芯片的3D微通道中单个海马神经元功能的探究 . | 科技通报 , 2020 , 36 (4) , 40-44,71 . |
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