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学者姓名:杨宏
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Abstract :
By embedding immobilized biofiller, 100% denitrification efficiency was achieved with nitrate or nitrite as electron acceptors utilizing volatile fatty acids (VFAs) from domestic wastewater after hydrolysis acidification. The consumption patterns of VFAs by functional bacteria and differences in nitrogen metabolic gene expression were thoroughly analyzed. Total consumption of acetic and propionic acids with >95% VFAs utilization was achieved utilizing nitrate, whereas the consumption of butyric and valeric acids was enhanced utilizing nitrite. Denitrification-related genes were all upregulated, particularly nosZ, indicating systemic N2O emission reduction potential. Electron acceptor changes dynamically shifted microbial dominance from Thauera (19.4%) to Thiobacillus (7.2%). These results provide valuable insights into the adaptability and ecological niche characterization of denitrifying bacteria, contributing to improving nitrogen removal efficiency, optimizing carbon source utilization, and reducing sludge production.
Keyword :
Microbial community dynamics Microbial community dynamics Nitrogen removal Nitrogen removal Organic carbon utilization Organic carbon utilization Immobilized technology Immobilized technology Denitrification electron acceptors Denitrification electron acceptors
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GB/T 7714 | Li, Siqi , Yang, Hong . Optimizing denitrification with volatile fatty acids from hydrolysis acidification-treated domestic wastewater: Comparative effects of nitrate and nitrite using immobilized biofiller [J]. | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2025 , 378 . |
MLA | Li, Siqi 等. "Optimizing denitrification with volatile fatty acids from hydrolysis acidification-treated domestic wastewater: Comparative effects of nitrate and nitrite using immobilized biofiller" . | JOURNAL OF ENVIRONMENTAL MANAGEMENT 378 (2025) . |
APA | Li, Siqi , Yang, Hong . Optimizing denitrification with volatile fatty acids from hydrolysis acidification-treated domestic wastewater: Comparative effects of nitrate and nitrite using immobilized biofiller . | JOURNAL OF ENVIRONMENTAL MANAGEMENT , 2025 , 378 . |
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Abstract :
The current sewage treatment systems are typically unable to efficiently remove both nitrogen and phosphorus simultaneously, often compromising biological phosphorus removal to ensure effective nitrogen removal. To resolve this issue, this study establishes an independent enhanced biological phosphorus removal (EBPR) system based on an immobilized fillers nitrogen removal system for treating actual rural sewage. The research focuses on the phosphorus removal performance, operational conditions, and characteristics of the independent EBPR system. When fluctuations occur in the effluent phosphorus concentration, phosphorus-accumulating organisms (PAOs) utilize only 29 % of the available carbon sources, with the remaining carbon being fully consumed by glycogen-accumulating organisms (GAOs). The presence of GAOs and low dissolved oxygen (DO) concentrations leads to deteriorating phosphorus removal performance in the system. To address this, reducing the carbon load and increasing aeration are necessary to maintain effluent phosphorus concentrations below 0.5 mg & sdot;L- 1. Tetrasphaera and Acinetobacter are identified as key microbial genera responsible for phosphate removal in this system. Finally, fluorescence excitation emission matrix parallel factor analysis (EEM-PARAFAC) showed that fermentation microorganisms, represented by Tetrasphaera, played a key role in the removal of tryptophan and tyrosine produced by raw wastewater and microbial metabolism.
Keyword :
Immobilized fillers Immobilized fillers Microbial community structure Microbial community structure Parallel factor analysis Parallel factor analysis Independent enhanced biological phosphorus removal Independent enhanced biological phosphorus removal
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GB/T 7714 | Chen, Wenhao , Yang, Hong , Wang, Xiaotong et al. Experimental study on the establishment of independent enhanced biological phosphorus removal system based on immobilized fillers for wastewater nitrogen removal [J]. | JOURNAL OF WATER PROCESS ENGINEERING , 2025 , 70 . |
MLA | Chen, Wenhao et al. "Experimental study on the establishment of independent enhanced biological phosphorus removal system based on immobilized fillers for wastewater nitrogen removal" . | JOURNAL OF WATER PROCESS ENGINEERING 70 (2025) . |
APA | Chen, Wenhao , Yang, Hong , Wang, Xiaotong , Liu, Weichao . Experimental study on the establishment of independent enhanced biological phosphorus removal system based on immobilized fillers for wastewater nitrogen removal . | JOURNAL OF WATER PROCESS ENGINEERING , 2025 , 70 . |
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Abstract :
A gel filler with diatomaceous earth, activated carbon, and CaCO3 compounded with polyvinyl alcohol was prepared and optimized. Rapid start-up and efficient and stable operation of partial nitrification (PN) was achieved by immobilization of conventional activated sludge. The results showed that the proper material proportion synergistically improved the performance and oxygen mass transfer rate of the gel. The continuous flow reactor was operated for 10d to successfully start PN, and the maximum ammonia oxidation rate of this filler was 58.30 mg center dot(L center dot h)- 1 during the stable operation, and the nitrite accumulation rate was maintained above 90 %. However, due to the greater mass transfer resistance of unoptimized fillers, more dissolved oxygen waste and nitrogen loss were caused, resulting in delayed expression of biological activity. Based on the kinetic characterization of ammonia-oxidizing bacteria (AOB) within the filler and high-throughput sequencing, it was demonstrated that the rapid start-up and efficient and stable operation of the PN system was due to the highly oxygenophilic and community-dominant position of AOB within this filler. This study provides new options and insights at the level of immobilization technology for the realization and efficient and stable operation of PN.
Keyword :
Immobilization materials Immobilization materials Partial nitrification Partial nitrification Nitrogen removal Nitrogen removal Start-up Start-up Oxygen mass transfer Oxygen mass transfer
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GB/T 7714 | Zhang, Xinming , Yang, Hong . Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration [J]. | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2024 , 12 (5) . |
MLA | Zhang, Xinming et al. "Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration" . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING 12 . 5 (2024) . |
APA | Zhang, Xinming , Yang, Hong . Optimize the ratio of immobilized gel materials to improve gel performance and oxygen mass transfer rate: Achieve rapid start-up and efficient and stable operation of partial nitration . | JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING , 2024 , 12 (5) . |
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Abstract :
A two-stage partial nitritation/anammox process based on microbial encapsulation (PN/A-E) was established. The nitrogen removal characteristics of PN/A-E under high and low ammonia nitrogen and after reactivation following a long-term shutdown were comprehensively investigated and compared with anammox granular sludge (AnGS). The stable PN process did not depend on high ammonia nitrogen, and the nitrite accumulation rate reached 95.2 +/- 0.7 %. The overall nitrogen removal rate of encapsulated anammox bacteria was twice that of the AnGS, and it was more tolerant to external interference. Moreover, PN/A-E showed good reactivation performance, and the total nitrogen in the effluent was 10.0 +/- 1.4 mg center dot L-1 when the final hydraulic retention time was 2.18 h. The immobilized fillers support an increase in ammonia-oxidizing bacteria under restricted conditions and were more conducive to the dominance of functional bacteria and the stability of microbial community under low ammonia nitrogen. This study provides a positive method to achieve a reliable PN/A.
Keyword :
Microbial community structure Microbial community structure Anammox Anammox Nitrogen removal Nitrogen removal Partial nitritation Partial nitritation Microbial immobilization Microbial immobilization
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GB/T 7714 | Wang, XiaoTong , Yang, Hong , Wang, JiaWei . Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes [J]. | BIORESOURCE TECHNOLOGY , 2023 , 370 . |
MLA | Wang, XiaoTong et al. "Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes" . | BIORESOURCE TECHNOLOGY 370 (2023) . |
APA | Wang, XiaoTong , Yang, Hong , Wang, JiaWei . Gel-immobilized partial nitritation/anammox achieves reliable nitrogen removal at different concentrations of nitrogen and reactivation processes . | BIORESOURCE TECHNOLOGY , 2023 , 370 . |
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Abstract :
The treatment performance of anaerobic ammonia oxidation (anammox) immobilized filler on different proportions of domestic wastewater was evaluated. The results showed that, in comparison to synthetic wastewater, 50% domestic wastewater promoted the anammox reaction of immobilized filler, while 100% domestic wastewater had no significant effect on the anammox activity of immobilized filler but the total nitrogen removal efficiency (TNRE) was improved through enhanced denitrification. The TNRE of the immobilized filler was 82.5%, which was significantly higher than that of AnGS (69.7%), and its average anammox contribution rate was more than 90%. This was because the encapsulated anammox biomass could better maintain competitive advantages and coordinate the symbiotic relationship with denitrifying bacteria. Moreover, lower NH4+-N concentration resulted in greater influence of C/N ratio on anammox performance than COD concentration, while the opposite was true at high NH4+-N concentration. This study verified that anammox immobilized filler is effective for mainstream applications.
Keyword :
Microbial composition Microbial composition Domestic wastewater Domestic wastewater Anammox Anammox Immobilized filler Immobilized filler C/N ratio C/N ratio
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GB/T 7714 | Wang, Xiao Tong , Yang, Hong , Zhou, YaKun et al. Performance and mechanism analysis of gel immobilized anammox bacteria in treating different proportions of domestic wastewater: a valid alternative to granular sludge [J]. | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
MLA | Wang, Xiao Tong et al. "Performance and mechanism analysis of gel immobilized anammox bacteria in treating different proportions of domestic wastewater: a valid alternative to granular sludge" . | BIORESOURCE TECHNOLOGY 347 (2022) . |
APA | Wang, Xiao Tong , Yang, Hong , Zhou, YaKun , Liu, XuYan . Performance and mechanism analysis of gel immobilized anammox bacteria in treating different proportions of domestic wastewater: a valid alternative to granular sludge . | BIORESOURCE TECHNOLOGY , 2022 , 347 . |
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Abstract :
To overcome the issue of substandard nitrogen and phosphorus discharge, resulting from the low carbon/ni-trogen (C/N) ratio of municipal wastewater, immobilized filler was used to re-hydrolyze the alkaline fermen-tation liquid of waste activate sludge (WAS) as a nitrogen removing carbon source. The complex organic matter was further hydrolyzed and its denitrification efficiency as a carbon source for nitrogen removal was improved. Volatile fatty acids increased from 1605 & PLUSMN; 5 mg/L to 2546 +/- 37 mg/L after re-hydrolysis of mixed-alkali sludge fermentation liquid, and complex organic matter, such as fulvic acids, humic acids, class I aromatic proteins, and soluble microbial products were hydrolyzed into small-molecule organics. When the re-hydrolysis fermentation liquid (RH-SFL) was used as the carbon source for nitrogen removal, the nitrogen removal effect was close to sodium acetate and the nitrogen removal rate (NRR) was up to 98.5 +/- 0.5%. According to the NOX--N removal curve, the reaction rate of RH-SFL was significantly higher than before re-hydrolysis (nRH-SFL) (0.486 > 0.38). This technology can significantly improve the effective organic quality in the alkaline fermentation liquid of WAS, which can be more easily utilized by denitrifying bacteria. This provides a feasible and effective solution for nitrogen removal in municipal wastewater with insufficient carbon.
Keyword :
Re-hydrolysis Re-hydrolysis Carbon source Carbon source Immobilized fillers Immobilized fillers Denitrification Denitrification Waste activated sludge Waste activated sludge
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GB/T 7714 | Liu, Xuyan , Yang, Hong , Chang, Jiang et al. Re-hydrolysis characteristics of alkaline fermentation liquid from waste activated sludge: Feasibility as a carbon source for nitrogen removal [J]. | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION , 2022 , 165 : 230-240 . |
MLA | Liu, Xuyan et al. "Re-hydrolysis characteristics of alkaline fermentation liquid from waste activated sludge: Feasibility as a carbon source for nitrogen removal" . | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION 165 (2022) : 230-240 . |
APA | Liu, Xuyan , Yang, Hong , Chang, Jiang , Bai, Yongsheng , Shi, Luyuan , Su, Bojun et al. Re-hydrolysis characteristics of alkaline fermentation liquid from waste activated sludge: Feasibility as a carbon source for nitrogen removal . | PROCESS SAFETY AND ENVIRONMENTAL PROTECTION , 2022 , 165 , 230-240 . |
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Abstract :
The immobilized carrier was prepared with complete anaerobic ammonia oxidation granular sludge (AnGS) and crushed AnGS, respectively. We evaluated the effects of granular form and continuous temperature changes on nitrogen removal by immobilized anaerobic ammonium oxidation (anammox) filler. The results showed that the rate of nitrogen removal of crushed and encapsulated AnGS was 20% higher than that of direct encapsulated AnGS. However, the latter had higher thresholds of tolerance to Fe2+ and Cu2+. In addition, the immobilization reduced the activation energy of anammox. Above 12.5 degrees C, the immobilized filler was efficient at removing nitrogen removal through the dual adjustment of temperature-hydraulic retention time. From 12.5 degrees C to 23 degrees C, the temperature had a greater influence on the nitrogen removal effect than the HRT. In contrast, HRT had a dominant influence from 23 degrees C to 32 degrees C. Anammox activity was severely inhibited below 12.5 degrees C. High-throughput sequencing analysis showed that the community structure migrated with the changes in temperature. The anammox functional bacteria Candidatus Kuenenia (18.31-39.73%) were the dominant genus at medium and high temperatures, and it was replaced by Chryseobacterium (24.19%) at 8.5 degrees C. In addition, an RDA analysis showed that Candidatus Brocadia was more adaptable to low temperatures than Candidatus Kuenenia. In addition, Bellilinea was more sensitive to temperature than Candidatus Kuenenia. Thus, the temperature could be appropriately lowered to avoid overbreeding. The results of this study optimized the operation of an anammox immobilized system and promote its further application. (C) 2021 Elsevier B.V. All rights reserved.
Keyword :
Anammox Anammox Temperature Temperature AnGS form AnGS form Metal ions Metal ions Microflora structure Microflora structure Immobilized filler Immobilized filler
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GB/T 7714 | Wang, XiaoTong , Yang, Hong , Su, Yang et al. Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
MLA | Wang, XiaoTong et al. "Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis" . | SCIENCE OF THE TOTAL ENVIRONMENT 803 (2022) . |
APA | Wang, XiaoTong , Yang, Hong , Su, Yang , Liu, XuYan . Effect of the form of granular sludge and temperature on anammox immobilized fillers: From performance to microbial community analysis . | SCIENCE OF THE TOTAL ENVIRONMENT , 2022 , 803 . |
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Abstract :
一种高积累率短程硝化活性污泥的培养方法,属于水处理领域。采用污水处理厂的活性污泥为种源,保持了原有短程硝化(AOB)菌群的结构特征,采用静置24h的FA抑制启动方法,帮助AOB菌群建立优势地位,并在高溶解氧(DO)的运行条件下,利用高FNA抑制了亚硝酸盐氧化菌(NOB)的生长,维持高积累率,再通过规律排泥,进而在保持系统高氨氧化速率的前提下,规律性排泥淘汰NOB,使AOB快速富集,实现高积累率短程硝化活性污泥的培养。
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GB/T 7714 | 杨宏 , 王泊含 . 一种高积累率短程硝化活性污泥的培养方法 : CN202210297622.X[P]. | 2022-03-23 . |
MLA | 杨宏 et al. "一种高积累率短程硝化活性污泥的培养方法" : CN202210297622.X. | 2022-03-23 . |
APA | 杨宏 , 王泊含 . 一种高积累率短程硝化活性污泥的培养方法 : CN202210297622.X. | 2022-03-23 . |
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Abstract :
一种高耐盐性的反硝化细菌菌群筛选培养方法,属于污、废水处理领域。以污水处理厂二沉池的回流污泥作为接种污泥,人工配制微生物所需要的碳源,氮源以及微量元素等物质,利用高浓度底物流加和间歇式运行实现对盐度值4%培养环境的建立与维持,对运行参数如pH,温度等进行严格的控制,并通过对出水各项指标的检测来调整进水负荷,以此成功筛选培养出一种高耐盐性的反硝化细菌菌群。
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GB/T 7714 | 杨宏 , 张鑫 . 一种高耐盐性的反硝化细菌菌群筛选培养方法 : CN202210297669.6[P]. | 2022-03-23 . |
MLA | 杨宏 et al. "一种高耐盐性的反硝化细菌菌群筛选培养方法" : CN202210297669.6. | 2022-03-23 . |
APA | 杨宏 , 张鑫 . 一种高耐盐性的反硝化细菌菌群筛选培养方法 : CN202210297669.6. | 2022-03-23 . |
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Abstract :
基于包埋生物填料的稀土矿山废水脱氮的装置与方法,属于污、废水处理领域。主要包括原水箱、原水泵、鼓风机、气体转子流量计、曝气盘、硝化反应装置、包埋硝化细菌填料、在线pH探头、PLC控制柜、第一加药泵、第一储药箱、第二加药泵、第二储药箱、管道混合器、反硝化反应装置、包埋反硝化细菌填料、最终出水口。以包埋硝化细菌填料为核心建立的硝化反应装置,以包埋反硝化细菌填料为核心建立的反硝化反应装置,其中硝化反应装置和反硝化反应装置中仅有包埋生物填料,没有活性污泥,生物化学作用完全依靠各反应装置的包埋生物填料。利用包埋生物填料能够形成更为高效和系统稳定运行的脱氮处理系统。
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GB/T 7714 | 杨宏 , 邹志强 . 基于包埋生物填料的稀土矿山废水脱氮的装置与方法 : CN202210297668.1[P]. | 2022-03-23 . |
MLA | 杨宏 et al. "基于包埋生物填料的稀土矿山废水脱氮的装置与方法" : CN202210297668.1. | 2022-03-23 . |
APA | 杨宏 , 邹志强 . 基于包埋生物填料的稀土矿山废水脱氮的装置与方法 : CN202210297668.1. | 2022-03-23 . |
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