To　investigate　the　effects　of　an　anaerobic　fermentation　method　on　milk-residue　wastewater　acidogenicity,　natural,　beaumontage,　and　mediosilicic　fermentation　(pH=7.0±0.2)　were　used　with　two　operation　cycles　continuously.　Different　chemical　and　biological　indicators,　such　as　soluble　chemical　oxygen　demand,　protein,　polysaccharides,　short-chain　fatty　acids　(SCFAs),　hydrolase　(protease,　α-glycosides　enzyme),　coenzyme　F420,　NH4+-N,　and　PO43-P,　were　analyzed　in　the　anaerobic　fermentation　process.　The　mediosilicic　fermentation　system　had　high　hydrolase　activity,　which　led　to　higher　hydrolysis　rates　of　protein　and　polysaccharides.　This　caused　higher　acidogenicity　performance,　and　the　maximum　SCFA　accumulation　was　12328.37　mg　COD·L-1.　However,　the　natural　fermentation　system　had　lower　hydrolase　activity,　and　large　amounts　of　protein　and　polysaccharides　were　left,　which　led　to　lower　SCFA　production;　the　minimum　value　was　4322.61　mg　COD·L-1.　Individual　SCFAs　showed　significant　differences　between　the　three　fermentation　systems,　where　the　maximum　accumulation　rate　of　acetic　acid　was　69.70%　in　the　natural　fermentation　system,　that　of　propionic　acid　was　49.27%　in　the　mediosilicic　fermentation　system,　and　that　of　butyric　acid　was　38.85%　in　the　beaumontage　fermentation　system.　©　2017,　Science　Press.　All　right　reserved.