To　avoid　the　inaccuracy　measurement　caused　by　the　different　temperature　between　calibration　solution　and　test　solution　in　actual　use　of　enzyme　injection　glucose　biosensor,　a　detection　method　was　established　for　enzyme　injection　glucose　biosensors　based　on　temperature.　Firstly,　the　concentration　detection　model　of　the　enzyme　injection　glucose　biosensor　is　established　according　to　enzymatic　reaction　kinetics.　Then,　the　relationship　between　the　temperature　and　the　unknown　parameters　in　the　dynamic　model　of　concentration　detection　is　built　using　Arrhenius　equation,　and　this　relationship　is　substituted　into　the　original　detection　dynamics　model　to　establish　the　new　model　based　on　temperature.　In　this　model,　the　current　initial　slope　of　the　enzymatic　reaction　and　the　temperature　are　the　inputs,　and　the　concentration　of　glucose　is　output.　Based　on　this　new　model,　a　detection　method　which　is　derived　by　the　temperature　of　the　mixture　and　the　slope　of　the　initial　current　is　proposed.　The　new　detection　method　can　not　only　reduce　the　impact　of　temperature　difference　to　improve　detection　accuracy,　but　also　avoid　the　removal　procedure　of　sampling　probe　causing　by　the　calibration　before　each　measurement,　and　it　is　more　conducive　to　online　use.　A　comparison　was　made　by　detecting　the　glucose　solution　with　a　concentration　of　1.　5　mg/mL,　2.　5　mg/mL　at　the　temperature　of　25,　30,　and　42　degrees　C　using　both　the　original　detection　method　and　the　new　detection　method　based　on　temperature.　The　result　shows　that　the　recovery　rate　of　the　new　method　is　more　than　95.　0%,　significantly　better　than　that　of　the　original　detection　method.