The　use　of　new　polymeric　building　block　is　one　of　the　important　routes　to　extend　the　layer-by-layer　(LbL)　assembly　technique　to　fabricate　novel　nanofiltration　(NF)　membrane.　In　this　work,　the　low　cost　sulfonated　cyclohexanone-formaldehyde　(SCF)　condensation　polymer　is　used　as　anionic　building　blocks　to　prepare　a　novel　positively　charged　nanofiltration　membrane　by　LbL　self-assembly　technique.　The　LbL　assembly　process　was　investigated　by　XRD,　FTIR,　SEM　and　AFM　measurements.　The　separation　performance　of　cationic　ions　and　dyes　was　evaluated　by　pressure-driven　nanofiltration　tests.　It　is　found　that　the　multilayer　surfaces　exhibited　periodic　variations　in　positive　charges.　And　the　prepared　membrane　showed　an　effective　rejection　of　both　cationic　ions　and　dyes.　The　multilayer　membrane　of　4.5　bilayers　exhibited　rejections　of　92.8%　and　90.6%　to　Ni2+　and　Ca2+,　along　with　flux　of　40.8　and　44.9　L/(m(2).h.MPa)　and　pure　water　flux　of　493　L/(m(2).h.MPa),　respectively.　Simultaneously,　this　membrane　also　showed　rejection　of　93.7%　and　90.8%　to　RdB　and　EbT,　along　with　flux　of　24.3　and　20.5　L/(m(2).h.MPa),　respectively.　Moreover,　the　long-term　performance　stability　of　the　membrane　was　improved　through　cross-linking　the　multilayer　membrane.　(C)　2015　Elsevier　B.V.　All　rights　reserved.