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目的通过建立新型人源化抗HIV-1 P24单克隆抗体制备技术平台,研制1~2种抗HIV-1 P24抗体。方法使用连接P24的磁珠分选特异性分泌P24抗体的B细胞,有限稀释后,提取总mRNA,采用逆转录和巢式PCR扩增免疫球蛋白重链和轻链基因,经测序鉴定后克隆到真核表达载体Cloning vector AbVec-hIgG1、Cloning vector AbVec-hIgKappa、Cloning vector AbVec-hIgLambda中;通过瞬时转染293T细胞得到重组抗体;使用proteinA亲和层析纯化抗体。结果成功筛选到5对抗HIV-1 P24的人源单克隆抗体基因。结论本研究初步...
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中华实验和临床病毒学杂志
Year: 2014
Issue: 05
Volume: 28
Page: 392-394
Cited Count:
WoS CC Cited Count: 0
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 13
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