To　study　the　inhibitory　effect　of　Hepatocyte　growth　factor　(HGF)　on　the　responsive　hyperplasia　of　damaged　astrocytes　in　vitro.　We　prepared　damaged　model　of　astrocytes　to　simulate　the　responsive　hyperplasia　of　damaged　astrocytes　in　vivo　by　culturing　astrocytes　in　vitro;　After　the　first　day　of　Ad-HGF　transfection,　astrocytes　were　scratched,　then　after　the　first,　the　third,　and　the　fifth　day　of　scratch,　we　detect　the　expression　amount　of　astrocytes　specific　glial　fibrillary　acidic　protein　(GFAP)　and　the　ratio　of　S-phase　cells　with　flow　cytometry,　both　of　which　can　reflect　the　proliferation　status　of　damaged　astrocytes;　After　HGF　was　added　in　scratched　astrocytes,　the　activity　of　SPK　and　MAPK　(P42/44)　were　detected　by　autoradiography　and　immunoblotting　test;　After　adding　different　concentrations　of　HGF　protein　in　astrocytes　cultured　in　different　serum　concentrations　and　adding　diverse　concentrations　of　HGF　protein,　SPK　and　SPK　inhibitor　DMS　in　scratched　astrocytes,　we　detect　cell　proliferation　with　3H-TDR　incorporation.　The　first　day　after　Ad-HGF　transfected　astrocytes　were　scratched,　the　amount　of　GFAP　secreted　by　astrocytes　were　decreased　significantly　(P　＜　0.05),　and　the　cells　in　S　phase　were　declined　obviously.　HGF　has　bidirectional　regulation　on　SPK　of　scratched　astrocytes:　increases　the　SPK　activity　when　HGF　in　low　dose,　while　inhibits　when　in　high　dose.　In　addition,　DMS　can　block　the　signal　passage;　HGF　had　no　effects　on　MAPK　(P42/44)　of　damaged　astrocytes　cells.　In　conclusion,　after　the　transfection　of　Ad-HGF,　it　can　inhibit　the　responsive　hyperplasia　of　damaged　astrocytes　by　the　means　of　blocking　SPK　passage.