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Abstract:
在TPA诱导永生化食管上皮细胞癌变中,NGAL(neutrophil gelatinase-associated lipocalin)过表达,提示食管癌细胞NGAL转录调控区可能存在TPA应答元件.为了对食管癌细胞NGAL的这一TPA应答元件进行分段定位鉴定,首先将NGAL5′侧翼区不同长度片段-1 431~+84-、1 137~+84、-945~+84、-657~+84、-416~+84和-152~+84等依次插入质粒pGLP,构建NGAL/pGLP系列报告基因荧光素酶表达载体pGLP-1431、pGLP-1137、pGLP-945、pGLP-657、pGLP-416和pGLP-152;然后...
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中国生物化学与分子生物学报
Year: 2006
Issue: 02
Page: 135-141
Cited Count:
WoS CC Cited Count: 0
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
WanFang Cited Count:
Chinese Cited Count:
30 Days PV: 4
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