Digital　reconstruction　of　3D　neuron　structures　is　an　important　step　toward　reverse　engineering　the　wiring　and　functions　of　a　brain.　Toward　this　end,　the　BigNeuron　project　bench　testing　was　launched　to　gather　a　worldwide　community　to　establish　a　Big　Data　resource　and　a　set　of　the　state-of-the-art　of　single　neuron　reconstruction　algorithms　for　neuroscience　community.　As　one　of　the　communities,　we　contribute　a　Mean　shift　and　Minimum　Spanning　Tree　(M-MST)　algorithm　to　trace　single　neuron　morphology.　In　our　experiment,　we　have　successfully　reconstructed　120　Drosophila　neurons　by　using　the　M-MST　algorithm　and　achieved　relatively　good　difference　scores　compared　with　other　four　algorithms　by　using　APP2　as　a　reference　object.