It　is　of　great　importance　to　develop　new　micro-actuators　with　high　performance　by　optimizing　the　structures　and　materials.　Here　we　develop　a　VO2/Al2O3/CNT　eccentric　coaxial　nanofiber,　which　can　be　potentially　applied　as　a　micro-actuator.　The　specific　eccentric　coaxial　structure　was　efficiently　fabricated　by　conventional　thin　film　deposition　methodology　with　individual　CNT　templet.　Activated　by　thermal　and　photothermal　stimuli,　the　as-developed　actuator　delivers　a　bidirectional　actuation　behavior　with　large　amplitudes　and　an　ultra-fast　response,　similar　to　2.5　mS.　A　tweezer　can　be　further　made　by　assembling　two　such　nanofibers　symmetrically　onto　a　tungsten　probe.　Clamping　and　unclamping　can　be　realized　by　laser　stimulus.　More　experimental　and　simulation　investigations　indicated　that　the　actuation　behaviors　could　be　attributed　to　the　nanostructured　eccentric　coaxial　geometry,　the　thermal　coefficient　mismatch　between　layers　and　the　fast　phase　transition　of　VO2.　The　micro-actuators　will　have　potentials　in　micro　manipulators,　nanoscaled　switches,　remote　controls　and　other　autonomous　systems.　Furthermore,　a　large　variety　of　coaxial　and　eccentric　coaxial　nanofibers　with　various　functions　can　also　be　developed,　giving　the　as-developed　methodology　more　opportunities.