Atmospheric　plasma　spraying　(APS)　is　widely　used　in　the　fabrication　of　hydroxyapatite　(HA)　coating　due　to　the　advantages　of　low-cost,　simple　operation　and　it　can　be　used　to　fabricate　coatings　with　controllable　structure.　Despite　all　these　advantages,　the　application　of　APS　deposited　HA　coating　for　long-term　use　is　limited　by　its　low　crystallinity,　low　phase　purity　and　poor　antibacterial　property　efficiency.　Herein,　HA　coatings　with　high　crystallinity,　phase　purity　and　c-axis　crystalline　orientation　were　fabricated　by　APS.　In　vitro　cell,　culture　testing　suggested　that　the　c-axis　oriented　HA　coating　was　more　effective　to　promote　the　proliferation　of　osteoblasts　cells　compared　with　other　coatings.　The　antibacterial　activity　of　HA　coating　against　the　bacteria　indicated　that　the　c-axis　oriented　HA　coating　restrained　the　bacterial　adhesion,　and　the　antibacterial　effect　of　the　E.　coli　bacteria　was　more　pronounced　than　that　of　the　S.　epidermidis　bacteria.　This　study　suggests　that　the　HA　coatings　with　the　specific　c-axis　crystalline　orientation　are　available　for　their　potential　applications　for　orthopedic　and　dental　implantation.　©　2021　Elsevier　B.V.