By　specific　oxygen　uptake　rate,　(SOUR)　measurements　of　activated　sludge　with　different　metal　ion　concentration,　the　optimal　stimulation　concentrations　of　Mn2+,　Mo6+　and　Zn2+　were　determined.　Then　within　each　metal　ion　stimulation　concentration　range,　extracellular　polymeric　substances　(EPS)　were　extracted　from　activated　sludge　and　its　components　(protein,　polysaccharide　and　nucleic　acid)　contents　changes　were　investigated.　The　results　showed　that　the　optimal　stimulation　concentration　of　each　metal　ion　is　1mg　x　L(-1).　EPS　components　of　activated　sludge　vary　obviously　with　Mn2+　and　Zn2+　concentration　change　but　not　with　Mo6+.　After　storage　at　low　temperature,　EPS　quantities　in　activated　sludge　are　reduced　with　polysaccharide　reduction　is　the　most.