Oil　spillages　lead　to　the　formation　of　hydrocarbon　and　metal　mixtures　possessing　effects　on　alkane-degrading　bacteria　that　are　responsible　for　the　bioremediation　of　oil-contaminated　soils　and　waters.　Studies　of　bacterial　responses　to　the　mixture　of　petroleum　and　metal　can　inform　appropriate　strategies　for　bioremediation.　We　employed　a　luminescent　bioreporter　Acinetobacter　baylyi　ADPWH_recA　with　alkane　degradation　capability　to　evaluate　the　combined　effects　from　heavy　metals　(Cd,　Pb　and　Cu)　and　alkanes　(dodecane,　tetradecane,　hexadecane　and　octadecane).　Bioluminescent　ratios　of　ADPWH_recA　in　single　Cd　or　Pb　treatments　ranged　from　0.25　to　1.98,　indicating　both　genotoxicity　and　cytotoxicity　of　these　two　metals,　while　ratios　1.0　postexposure　to　Cu　showed　its　cytotoxic　impacts　on　ADPWH_recA　bioreporter.　Metal　mixtures　exhibited　enhanced　antagonistic　effects　(Ti　20%,　showing　the　inhibition　of　alkanes　on　Cd　toxicity.　Exposed　to　the　metal　mixture　containing　10　mg/L　Cu,　the　weak　binding　affinity　of　Cu　with　alkanes　contributed　to　a　high　morbidity　of　85%　in　ADPWH_recA　cells.　This　study　provides　a　new　way　to　understand　the　toxicity　of　mixture　contaminants,　which　can　help　to　optimize　treatment　efficiencies　of　bacterial　remediation　for　oil　contamination.