N-6-methyladenosine　(m(6)A)　modification-the　most　prevalent　mammalian　RNA　internal　modification-plays　key　regulatory　roles　in　mRNA　metabolism.　Current　approaches　for　m(6)A　modified　RNA　analysis　limit　at　bulk-population　level,　resulting　in　a　loss　of　spatiotemporal　and　cell-to-cell　variability　information.　Here　we　proposed　a　m(6)A-specific　in　situ　hybridization　mediated　proximity　ligation　assay　(m(6)AISH-PLA)　for　cellular　imaging　of　m(6)A　RNA,　allowing　to　identify　m(6)A　modification　at　specific　location　in　RNAs　and　image　m(6)A　RNA　with　single-cell　and　single-molecule　resolution.　Using　m(6)AISH-PLA,　we　investigated　the　m(6)A　level　and　subcellular　location　of　HSP70　RNA103-m(6)A　in　response　to　heat　shock　stress,　and　found　an　increased　m(6)A　modified　ratio　and　an　increased　distribution　ratio　in　cytoplasm　under　heat　shock.　m(6)AISH-PLA　can　serve　in　the　study　of　m(6)A　RNA　in　single　cells　for　deciphering　epitranscriptomic　mechanisms　and　assisting　clinical　diagnosis.