This　study　aims　to　investigate　the　effect　of　Iran　rock　asphalt　(IRA)　on　the　rheological　performance　of　waste　bio-oil　modified　asphalt　(WBMA).　Three　kinds　of　WBMA　with　bio-oil　origins　including　soybean　oil　residue,　castor　oil　residue,　and　waste　cooking　oil　residue　were　blended　with　different　dosages　of　IRA.　The　high-temperature　performance　of　the　three　blended　asphalt　binders　was　evaluated　using　the　temperature　sweep　test　and　multiple　stress　creep　recovery　(MSCR)　test.　The　effect　of　IRA　on　the　fatigue　performance　and　low-temperature　cracking　resistance　of　the　three　WBMAs　was　evaluated　using　the　linear　amplitude　sweep　(LAS)　test　and　the　bending　beam　rheometer　(BBR)　test.　A　gel　permeation　chromatography　(GPC)　test　was　conducted　to　further　understand　the　contribution　of　IRA　to　the　overall　rheological　performance　of　different　WBMAs.　Results　indicated　that　the　high-temperature　performance　of　the　three　WBMAs　was　enhanced　significantly　with　the　increase　of　IRA　content.　The　predicted　fatigue　life　from　the　LAS　test　showed　that　the　incorporation　of　IRA　extended　the　fatigue　life　of　the　three　WBMAs　at　a　lower　strain　level,　while　this　effect　was　not　profound　at　a　higher　strain　level.　Results　from　BBR　test　showed　that　IRA　may　increase　the　risk　of　low-temperature　cracking　and　that　the　dosage　of　IRA　should　be　limited　to　fulfill　the　requirements　of　the　low-temperature　performance.　The　GPC　analysis　revealed　that　the　enhanced　high-temperature　performance　and　reduced　low-temperature　cracking　resistance　of　WBMAs　modified　with　IRA　was　mainly　a　result　of　the　increased　asphaltene　concentration.