The　process　of　starting　AAOB　granular　sludge　under　low　temperature　substrate　(NH4+-N,　NO2--N　(4010)　mg/L)　was　investigated.　In　this　study,　SBR　reactor　was　used　to　gradually　shorten　the　experimental　cycle　to　start　AAOB　granular　sludge,　and　the　nitrogen　removal　rate　and　nitrogen　load　of　the　system　were　analyzed　for　the　kinetic　characteristics　of　anaerobic　ammonium　oxidation　process　at　each　stage.　Meanwhile,　the　granular　sludge　morphology,　average　particle　size,　EPS　content,　and　three-dimensional　fluorescence　characteristics　of　EPS　were　analyzed.　Results　showed　that　the　experimental　period　was　shortened　(24　h12　h10　h8　h),　and　the　nitrogen　load　increased　from　0.074　g/(Ld)　to　0.204　g/(Ld).　The　Grau　second-orders　model　better　characterized　the　process　of　starting　AAOB　granular　sludge.　The　matrix　removal　rate　constant　k2　increased　from　0.15　to　0.22,　and　the　denitrification　performance　was　gradually　improved.　The　MLSS　and　MLVSS　of　the　sludge　were　finally　stabilized　from　2　989　and　2　348　mg/L　to　2　460　and　1　776　mg/L.　As　the　reaction　went　on,　the　anaerobic　ammonium　oxide　sludge　fully　utilized　the　matrix,　and　the　granular　sludge　was　gradually　stabilized.　The　average　particle　size　of　the　particles　reached　410　μm.　The　PN　content　and　the　PN/PS　value　first　increased　and　then　tended　to　be　stable,　and　they　became　larger　as　the　particle　size　increased.　The　three-dimensional　fluorescence　component　in　the　sludge　EPS　was　analyzed　by　the　PARAFAC　method,　which　was　composed　of　NADH,　riboflavin,　and　humic　acid.　Copyright　©2020　Journal　of　Harbin　Institute　of　Technology.All　rights　reserved.