Triclosan　(TCS)　and　copper　(Cu2+)　were　exposed　to　aerobic　granular　sludge　(AGS)　system　treating　wastewater　containing　environmental　tetracycline,　respectively,　to　explore　the　different　biochemical　responses,　more　importantly,　the　fates　of　resistance　genes　(RGs)　in　AGS　system.　The　results　showed　that　TCS　and　Cu2+　could　significantly　inhibit　the　N　and　P　removal　in　AGS　system　by　reducing　several　key　functional　genes,　including　amoA　gene　of　ammonia-oxidizing　bacteria,　Nitrospira　and　phosphorus　accumulating　organisms　16S　rRNA　genes.　TCS　caused　higher　degree　of　RGs＇　enrichment　than　Cu2+,　which　made　the　average　total　relative　abundance　of　RGs　of　1.38　+/-　0.73　and　0.78　+/-　0.24　in　TCS　.　and　Cu　system,　respectively.　Cu2+　could　induce　a　wider　range　of　horizontal　gene　transfer　than　TCS,　leading　to　the　detections　of　more　potential　hosts　harboring　RGs　in　Cu　system.　Cu　system　seemed　to　have　stronger　repair,　immunity　and　defense　ability　than　TCS　system,　which　enabled　it　to　have　sufficient　ability　to　trigger　protection　mechanism　to　realize　self-protection,　eventually　the　RGs　also　were　controlled.　Integron　(intI1　and　intI3)　and　plasmids　Orb-C　and　IncQ)　might　cooperate　with　microorganisms　and　water　quality　parameters　to　enhance　the　enrichment　of　RGs　in　TCS　system,　however　this　interaction　among　various　environmental　factors　was　not　obvious　in　Cu　system,　which　might　be　responsible　for　the　lower　abundance　of　RGs.　The　increasing　levels　of　Tcs　and　Cu2+　in　wastewater　should　be　paid　more　attentions　during　the　treatment　of　wastewater　containing　environmental　tetracycline　by　AGS　system.　Especially　for　TCS,　it　had　the　ability　to　enrich　RGs　more　easily　than　Cu2+,　which　should　be　prevented　from　entering　wastewater　treatment　plants　as　far　as　possible,　to　avoid　more　serious　proliferation　and　dissemination　of　various　RGs.