This　study　aimed　to　explore　the　impacts　of　ciprofloxacin　(CIP,　0.05-40　mg/L)　and　copper　(3　mg/L)　combined　pollution　on　nitrification,　microbial　community　and　antibiotic　resistance　genes　(ARGs)　in　activated　sludge　system　during　stress-and　post-effect　periods.　Higher　CIP　concentration　inhibited　nitrification　and　an　average　of　50%　total　nitrogen　removal　occurred　under　40　mg/L　of　CIP　pressure.　The　stress-and　post-effects　on　bacterial　diversity　and　structure　were　obviously　distinct.　Abundant　genera　were　more　sensitive　to　combined　pollution　than　rare　genera　based　on　full-scale　classification　and　conditionally　rare　or　abundant　taxa　were　keystone　taxa　in　their　interactions.　Ammonia　oxidation　genes　were　inhibited　under　high　CIP　level,　but　some　aerobic　denitrifying　bacteria　(Thauera,　Comamonas　and　Azoarcus)　and　key　genes　increased.　96　ARG　subtypes　were　detected　with　complex　positive　relationships　and　their　potential　hosts　(abundant-rare-functional　genera)　changed　in　two　periods.　This　study　highlights　the　different　stress-and　post-effects　of　combined　pollution　on　activated　sludge.