The　capture　and　manipulation　of　single　cells　are　an　important　premise　and　basis　for　intracellular　delivery,　which　provides　abundant　molecular　and　omics　information　for　bio-medical　development.　However,　for　intracellular　delivery　of　cargosinto/from　small-size　suspended　living　single　cells,　the　capture　methods　are　limited　by　the　lack　of　small-size　holding　pipets,　poor　cell　activity,　and　the　low　spatial　accuracy　of　intracellular　delivery.　To　solve　these　problems,　a　method　for　the　controllable　fabrication　of　small-size　holding　pipets　was　proposed.　A　simple,　homemademicroforge　instrument　including　an　imaging　device　was　built　to　cut　and　melt　the　glass　capillary　tip　by　controlling　the　heat　production　of　a　nichrome　wire.　The　controllable　fabrication　of　small-sizeholding　pipets　was　realized　by　observing　the　fabrication　process　in　real　time.　Combined　with　an　electroosmotic　drive　system　and　amicromanipulation　system　with　high　spatial　resolution,　the　holding　pipet　achieved　the　active　capture,　movement,　and　sampling　ofsuspended　living　single　cells.　Moreover,　solid-phase　microextraction　was　performed　on　captured　single　pheochromocytoma　cells,　and　the　extracted　dopamine　was　successfully　detected　using　an　electrochemical　method.　The　homemade　microforge　instrumentovercame　the　limitations　of　traditional　microforges,　resulting　in　holding　pipets　that　were　sufficiently　small　for　small-size　suspended　single　living　cells　(5-30　mu　m).　This　proactive　capture　method　overcame　the　shortcomings　of　existing　methods　to　achieve　the　multiangle,　high-precision　manipulation　of　single　cells,　thereby　allowing　the　intracellular　delivery　of　small-size　single　cells　in　suspension　with　high　spatiotemporal　resolution