The　treatment　performance　of　anaerobic　ammonia　oxidation　(anammox)　immobilized　filler　on　different　proportions　of　domestic　wastewater　was　evaluated.　The　results　showed　that,　in　comparison　to　synthetic　wastewater,　50%　domestic　wastewater　promoted　the　anammox　reaction　of　immobilized　filler,　while　100%　domestic　wastewater　had　no　significant　effect　on　the　anammox　activity　of　immobilized　filler　but　the　total　nitrogen　removal　efficiency　(TNRE)　was　improved　through　enhanced　denitrification.　The　TNRE　of　the　immobilized　filler　was　82.5%,　which　was　significantly　higher　than　that　of　AnGS　(69.7%),　and　its　average　anammox　contribution　rate　was　more　than　90%.　This　was　because　the　encapsulated　anammox　biomass　could　better　maintain　competitive　advantages　and　coordinate　the　symbiotic　relationship　with　denitrifying　bacteria.　Moreover,　lower　NH4+-N　concentration　resulted　in　greater　influence　of　C/N　ratio　on　anammox　performance　than　COD　concentration,　while　the　opposite　was　true　at　high　NH4+-N　concentration.　This　study　verified　that　anammox　immobilized　filler　is　effective　for　mainstream　applications.