• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
搜索

Author:

Zhang, Xiangchun (Zhang, Xiangchun.) | Zheng, Qinqin (Zheng, Qinqin.) | Wang, Ziqi (Wang, Ziqi.) | Xu, Chao (Xu, Chao.) | Han, Haolei (Han, Haolei.) | Li, Aiping (Li, Aiping.) | Ma, Guicen (Ma, Guicen.) | Li, Jiaojiao (Li, Jiaojiao.) | Lu, Chengyin (Lu, Chengyin.) | Chen, Hongping (Chen, Hongping.) | Zhang, Zhichao (Zhang, Zhichao.)

Indexed by:

Scopus SCIE

Abstract:

Tumor invasion/metastasis is still the major cause of death in cancer patients. Membrane type-1 matrix metalloproteinase (MT1-MMP) is directly related to tumor invasion/metastasis. To accurately and quickly distinguish the risk of invasion/metastasis of primary tumor cells, it is urgent to develop a simple and precise quantitative method to distinguish the expression level of MT1-MMP. In this work, we have constructed red fluorescent Au clusters with peroxidase-like properties that could specifically bind to MT1-MMP on human cervical cancer cells. After MT1-MMP was labelled with Au clusters, we could visually see red fluorescence of MT1-MMP on cervical cancer cells via fluorescence microscopy and catalytic color imaging using an ordinary optical microscope. The constructed Au clusters contained 26 Au atoms; thus, the amount of MT1-MMP on cervical cancer cells could be accurately quantified using inductively coupled plasma mass spectrometry (ICP-MS). More importantly, the invasion/metastasis capabilities of the cervical cancer Siha, Caski and Hela cells with different MT1-MMP amounts could be accurately distinguished by fluorescence/catalysis qualitative imaging and ICP-MS quantitative analysis. This method of qualitative/quantitative analysis of tumor-associated proteins on cancer cells has great potential for accurately diagnosing aggressive tumor cells and assessment of their invasion/metastasis risk.

Keyword:

Au clusters human cervical cancer invasion MT1-MMP metastasis

Author Community:

  • [ 1 ] [Zhang, Xiangchun]Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China
  • [ 2 ] [Zheng, Qinqin]Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China
  • [ 3 ] [Wang, Ziqi]Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China
  • [ 4 ] [Han, Haolei]Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China
  • [ 5 ] [Li, Aiping]Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China
  • [ 6 ] [Ma, Guicen]Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China
  • [ 7 ] [Lu, Chengyin]Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China
  • [ 8 ] [Chen, Hongping]Chinese Acad Agr Sci, Tea Res Inst, Hangzhou 310008, Peoples R China
  • [ 9 ] [Xu, Chao]Shandong Agr Univ, Coll Chem & Mat Sci, Tai An 271018, Shandong, Peoples R China
  • [ 10 ] [Li, Jiaojiao]Beijing Univ Technol, Fac Environm & Life Sci, Dept Chem & Biol, Beijing 100124, Peoples R China
  • [ 11 ] [Zhang, Zhichao]Fudan Univ, Shanghai Canc Ctr, Dept Musculoskeletal Tumor, Shanghai 200032, Peoples R China

Reprint Author's Address:

Show more details

Related Keywords:

Source :

NANOMATERIALS

Year: 2022

Issue: 1

Volume: 12

5 . 3

JCR@2022

5 . 3 0 0

JCR@2022

ESI Discipline: MATERIALS SCIENCE;

ESI HC Threshold:66

JCR Journal Grade:1

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count: 6

SCOPUS Cited Count: 10

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 4

Affiliated Colleges:

Online/Total:559/10582820
Address:BJUT Library(100 Pingleyuan,Chaoyang District,Beijing 100124, China Post Code:100124) Contact Us:010-67392185
Copyright:BJUT Library Technical Support:Beijing Aegean Software Co., Ltd.