A　continuous　plug-flow　reactor　with　anaerobic/front-aerobic/anoxic/post-aerobic　zones,　where　partial　nitrifi-cation　occurred　in　the　front-aerobic　zone,　followed　by　simultaneous　anammox　and　endogenous　denitrification　in　the　anoxic　zone　(PN-SAED),　was　built　up　to　treat　municipal　wastewater.　Alternating　anoxic/aerobic　conditions　and　longer　anoxic　duration　facilitated　stable　partial　nitrification.　The　nitrite　accumulation　ratio　(NAR)　was　maintained　at　97.4　+/-　1.2%,　with　temperatures　between　13.3　degrees　C　to　19.8　degrees　C.　Candidatus　Brocadia　were　naturally　enriched　in-situ　from　the　anoxic　zone　with　relative　abundances　of　31.93%　and　6.67%　on　the　agitator　blade　and　carriers,　respectively.　High　removal　efficiencies　of　total　inorganic　nitrogen　(TIN)　(95.1　+/-　1.9%)　and　effluent　TIN　(2.6　+/-　1.1　mg　N/L)　were　acquired　from　low　COD/TIN　(3.4　+/-　0.4)　municipal　wastewater　with　anammox　contribution　of　13.5%　+/-　5.8%　to　TIN　removal.　The　PN-SAED　process　is　a　promising　mainstream　nitrogen　removal　method.