Comprehensive　Summary　Chromatography　theory　shows　that　when　the　inner　diameter　of　the　chromatography　column　is　very　narrow　(e.g.,　1-2　mu　m),　the　maximum　efficiency　will　be　produced　in　liquid　chromatography.　In　addition,　the　increasing　demand　for　ultrasmall　volume　sample　analysis　has　produced　the　trend　of　narrowing　the　diameter　of　the　liquid　chromatography　column.　Experiments　have　verified　that　ultranarrow-bore　open　tubular　liquid　chromatography　(i.d.　＜=　2　mu　m)　has　the　advantages　of　small　sample　requirement　and　high　separation　efficiency.　However,　there　also　exist　huge　challenges　along　with　such　obvious　advantages.　This　review　summarizes　the　efforts　made　by　our　research　group　and　other　research　groups　to　develop　this　field.　We　hope　that　in　the　near　future,　ultranarrow　bore　open-tube　liquid　chromatography　can　be　successfully　and　maturely　applied　to　the　application　of　analytes　with　ultrasmall　size　and　volume　such　as　single-cell　and　even　subcellular　organelles　omics　research.　What　is　the　most　favorite　and　original　chemistry　developed　in　your　research　group?　Our　research　work　originated　from　nanocapillary　chromatographic　analysis,　and　then　expanded　to　micro/nanoscale　bio-separation　analysis,　micro/nanofluidic　analysis,　and　the　development　of　new　micro/nano　analytical　instruments.　Currently,　our　research　focuses　on　single-cell　analysis　with　temporal　and　spatial　resolution.　What　is　the　most　important　personality　for　scientific　research?　Having　an　enquiring　mind;　Be　diligent,　curious,　and　persistent;　Pursuing　perfection.　What＇s　your　hobbies?　What＇s　your　favorite　book(s)?　I　enjoy　cooking　and　playing　Ping-Pong.　My　favorite　book　is　The　Art　of　War.　Who　influences　you　mostly　in　your　life?　My　parents.　What　is　your　favorite　journal(s)?　Journal　of　the　American　Chemical　Society,　which　is　the　top　international　academic　journal　in　the　field　of　chemistry　and　values　novelty.　Could　you　please　give　us　some　advices　on　improving　Chinese　Journal　of　Chemistry?　Pursue　high　quality,　highlight　the　characteristics,　strive　for　excellence,　enable　fast　review　and　publication.