A　method　for　the　determination　of　19　mycotoxins　in　wheat　and　corn　by　high　performance　liquid　chromatography-tandem　mass　spectrometry　was　established.　After　the　sample　was　extracted　with　acetonitrile:　water:　formic　acid　(80:18:2)　solution,　centrifuged,　and　concentrated,　it　was　separated　on　a　C18　chromatographic　column,　methanol-formic　acid　aqueous　solution　was　used　as　mobile　phase　gradient　elution,　and　electrospray　positive　and　negative　ions　(ESI+,　ESI-)　were　used.　Simultaneous　ionization,　multi-reaction　monitoring　mode　detection,　and　isotope　internal　standard　method　for　quantification.　All　the　19　mycotoxins　had　a　good　linear　relationship　within　a　certain　content　range　(R2≥0.992),　the　limit　of　quantification　was　0.12~30　μg/kg,　and　the　recovery　rate　was　61%~117%,　under　the　three　levels　of　low,　medium　and　high　additive　concentration,　the　relative　standard　deviation　was　less　than　15%(n=6),　which　met　the　performance　requirements　of　daily　testing　methods.　This　method　was　easy　to　operate,　had　a　high　sensitivity　and　a　good　reproducibility,　and　was　suitable　for　the　simultaneous　determination　of　multiple　mycotoxins　in　wheat,　corn　and　other　grains.　©　2022,　Dept.　of　JCCOA.　All　right　reserved.