Surface　enhanced　Raman　scattering　(SERS)　is　an　ultrasensitive　trace　analysis　technique,　and　Raman　enhancement　is　mainly　contributed　by　surface　plasmon　resonance　of　the　metal　nanoparticles　immobilized　on　the　substrate.　Among　them,　flexible　SERS　substrates　are　more　suitable　for　a　wide　range　of　applications,　and　people　are　more　interested　in　flexible　substrates.　This　work　includes　preparation　of　transparent　flexible　SERS　substrate,　preparation　of　different　morphologies　of　silver　nanoparticles,　deposition　of　silver　nanoparticles　on　flexible　substrate　and　Raman　detection.　©　2022　SPIE.