In　recent　years,　non-motorized　vehicle　(NMV)　groups　for　the　purpose　of　avoiding　traffic　jams　and　facilitating　travel　have　developed　rapidly　in　urban　traffic.　They　share　limited　right-of-way　on　urban　roads,　which　leads　to　more　conflicts　between　each　other　and　increasing　accident　risks.　In　this　research,　the　traffic　flow　characteristics　of　three　types　of　NMV,　namely　bicycles　(B),　electric　bicycles　(EB),　and　electric　tricycles　(ET),　on　three　independent　bicycle　lanes　in　Beijing　were　extracted　by　two-dimensional　trajectory　tracking　method.　The　relationship　between　different　traffic　conflicts　and　traffic　density　and　anti-riding　rate　is　studied.　Results　indicate　two　remarkable　findings:　(1)　densities　of　B,　EB,　and　ET,　which　are　mostly　influenced　by　speed,　are　correlated　with　different　types　of　conflicts;　(2)　the　EB　conflict　shows　a　strong　linear　correlation　with　EB　density.　The　linear　prediction　model　of　opposing　conflict　is　established　using　reverse　riding　ratios　of　B,　EB,　and　ET.　This　research　recommends　that　reverse　riding　of　NMV　should　be　eliminated　to　reduce　the　bicycle　lane　conflict.　And　unidirectional　non-motorized　roads　with　physical　isolation　are　proposed　for　construction　in　urban　areas　with　large　non-motorized　traffic　flow.　Moreover,　the　travel　speed　of　NMV　on　bicycle　lane　should　be　further　regulated　to　provide　safe　and　friendly　riding　environment　for　riders.　©　2022,　The　Author(s),　under　exclusive　license　to　Springer　Nature　Singapore　Pte　Ltd.