The　effects　of　two　typical　heavy　metal　ions　[Cu()　and　Ni()]　and　humic　acid　on　ANAMMOX　nitrogen　removal　(SAA)　were　studied　through　batch　experiments,　and　the　kinetic　model　was　analyzed.　At　the　same　time,　the　effects　of　humic　acid-heavy　metal　on　ANAMMOX　nitrogen　removal　were　discussed.　The　results　showed　that　ANAMMOX　was　promoted　when　ρ[Cu()]　and　ρ[Ni()]　were　3　mg•L-1,　and　SAA　was　increased　by　8.64%　and　7.78%,　respectively;　ANAMMOX　was　inhibited　when　the　ρ[Cu()]　and　ρ[Ni()]　were　20　mg•L-1　and　5　mg•L-1,　respectively,　and　the　inhibition　effect　was　more　significant　with　the　increase　in　heavy　metal　ion　concentration.　The　index　fitting　showed　that　the　IC50　of　Cu()　and　Ni()　on　ANAMMOX　were　29.67　mg•L-1　and　28.75　mg•L-1,　respectively.　SAA　was　increased　by　7.37%　when　the　ρ(humic　acid)　was　1　mg•L-1,　and　the　inhibition　rate　reached　36.80%　when　the　humic　acid　concentration　was　40　mg•L-1.　The　linear　fitting　showed　that　the　IC50　of　humic　acid　on　ANAMMOX　was　58.36　mg•L-1.　The　modified　Michaelis-Menten　model　could　better　describe　the　inhibitory　kinetic　behavior　of　heavy　metals　and　humic　acid　on　ANAMMOX.　The　model　fitting　showed　that　the　complete　inhibition　concentrations　(I*)　of　Cu(),　Ni(),　and　humic　acid　on　ANAMMOX　were　49.59,　74.46,　and　84.27　mg•L-1,　respectively.　An　appropriate　amount　of　humic　acid　was　beneficial　to　improve　the　inhibition　of　heavy　metals　on　ANAMMOX　bacteria　activity,　and　excessive　humic　acid　would　cause　inhibition　on　ANAMMOX　bacteria　again.　©　2022,　Science　Press.　All　right　reserved.