Ultra-High　Performance　Concrete　(UHPC)　is　a　new　type　of　cement-based　composite　material　that　exhibits　exceptional　compressive　strength,　tensile　properties　and　durability　compared　to　ordinary　concrete.　Comprehensive　research　on　the　flexural　fatigue　behavior　of　prestressed　UHPC　beams　is　presented　in　this　paper.　The　failure　mode　of　the　fatigue　beam　is　the　fatigue　fracture　of　the　tensile　steel　bar　in　the　pure　bending　section.　In　the　fatigue　process,　the　fatigue　behaviors　of　prestressed　UHPC　beams　including　crack　width,　midspan　deflection,　the　strain　of　UHPC　in　compression　zone　and　strain　of　high-strength　steel　bars　exhibit　two　stages　which　are　corresponding　to　damage　initiation,　steady　damage　propagation.　The　residual　deflection　at　the　midspan　increases　with　the　increase　of　fatigue　cycles.　The　prestressed　UHPC　beam　reinforced　with　high-strength　steel　bars　shows　stable　structural　behavior　even　after　concrete　cracking.　The　S–N　expression　of　prestressed　UHPC　beam　with　consideration　of　the　influence　of　stress　amplitude　of　high-strength　steel　bars　is　proposed.　The　stiffness　degradation　formula　of　the　fatigue　beam　and　calculation　methods　of　fatigue　crack　width　and　fatigue　deflection　are　proposed.　©　2022　Institution　of　Structural　Engineers