The　application　of　partial　nitrification-anammox　(PNA)　in　continuous　flow　processes　for　treating　low　COD/N　(C/N)　sewage　remains　a　critical　challenge.　Here,　a　traditional　continuous　anoxic/oxic　(A/O)　process　was　operated　to　investigate　nitrogen　removal　from　municipal　wastewater　by　the　bio-augmentation　of　partial　nitrification　sludge　combined　with　the　inoculation　of　biocarriers　under　decreasing　temperatures.　Stable　enhanced　nitrogen　removal　via　PNA　was　achieved.　The　average　total　inorganic　nitrogen　in　influent　and　effluent　was　44.3　and　7.1　mg　N/L　under　a　low　C/N　ratio　(3.4)　and　a　short　hydraulic　retention　time　(8.2　h).　The　bio-augmentation　of　partial　nitrification　sludge　enhanced　the　PNA　process　under　low　temperatures　(16.9　+/-　0.6　?C).　The　nitrogen　removal　efficiency　remained　stable　at　83.3　+/-　5.7　%　as　the　temperature　decreased　from　29.1　to　16.3　?C,　and　the　relative　abundance　of　Ca.　Brocadia　in　carrier　biofilms　increased　from　2.22　%　to　4.31　%　and　3.27　%　in　two　aerobic　chambers　after　70　of