Separation　of　natural　products　from　their　mixture　of　structurally　similar　compounds　is　an　important　process　for　drug　development　and　application.　Borneol　is　a　natural　product　widely　used　in　the　treatment　of　the　cardiovascular　and　cerebrovascular　diseases,　and　its　production　always　involve　the　challenging　separation　from　the　diastereomer　isoborneol　(about　50　%).　Herein,　elven　types　of　metal-organic　frameworks　(MOFs)　with　diverse　pore　structure　and　functionalities　(-F,　-NH2,　-SO3H,　and　-OH)　are　utilized　to　evaluate　their　borneol/isoborneol　separation　performance,　as　the　first　attempt　to　use　MOFs　for　diastereomers　separation.　Among　these　MOFs,　UiO66-F4　exhibits　the　best　performance　with　the　borneol/isoborneol　selectivity　of　2.23　and　the　maximum　borneol　adsorption　capacity　of　106.88　mg　center　dot　g　1　at　298　K,　owing　to　the　efficient　pore　confinement　of　borneol　molecule　confirmed　by　molecular　simulation.　Subsequent　column　breakthrough　experiment　demonstrates　good　separation　performance　under　dynamic　conditions.　After　five-stage　purification　unit　process,　high-purity　borneol　(＞96　%)　can　be　obtained.　Furthermore,　good　reusability　and　scale-up　synthesis　suggest　a　high　potential　of　the　UiO-66-F4　materials　for　practical　application.