To　save　external　carbon　source　dosage　and　simplify　NH4+　to　NO2-　ratio　control　strategy,　this　study　established　a　novel　step-draining　based　partial　nitrification-denitrification　and　Anammox　(PND-AMX)　system　for　advanced　nitrogen　removal　from　mature　landfill　leachate.　Separation　of　partial　nitrification　and　denitrification　was　real-ized　based　on　step-draining,　achieving　74.8　%　nitrogen　removal.　25　%　was　the　optimal　volume　exchange　ratio　for　synergistic　removal　of　organics　and　nitrogen,　allowing　full　use　of　carbon　source.　NH4+　to　NO2-　ratio　was　easily　controlled　by　varying　the　volume　ratio　of　the　first　and　second　effluent　of　PND　reactor.　Brocadia,　Kuenenia　and　Jettenia　collectively　accounted　for　13.61　%　in　AMX　reactor,　contributing　21.0　%　of　nitrogen　removal.　Nitrogen　removal　efficiency　and　nitrogen　removal　rate　reached　98.3　+/-　1.2　%　and　3.07　+/-　0.09　kgN/(m(3).d),　respectively.　Partial　Anammox　process　based　on　step-draining　was　easier　to　realize　and　of　practical　significance　for　application　in　treatment　of　landfill　leachate.