Personal　protective　behaviors　of　healthcare　workers　(HCWs)　and　dynamic　changes　in　them　are　known　to　play　a　major　role　in　the　hospital　transmission　of　severe　acute　respiratory　syndrome　coronavirus　2　(SARS-CoV-2).　In　this　study,　1,499　HCWs　in　Chinese　hospitals　completed　an　online　survey　about　their　knowledge　on　SARS-CoV-2　transmission　and　their　personal　protective　behaviors　before　and　after　coronavirus　disease　2019　(COVID-19)　vaccination.　Of　all　the　respondents,　89%　were　vaccinated　at　the　time　of　the　survey　and　96%　believed　that　the　vaccine　was　effective　or　highly　effective.　Further,　88%　of　the　vaccinated　HCWs　expressed　that　they　would　get　revaccinated　if　the　vaccination　failed.　Compared　with　HCWs　with　a　lower　education　level,　those　with　a　higher　education　level　had　less　fear　of　being　infected　with　SARS-CoV-2　and　reported　a　lower　negative　impact　of　the　pandemic　on　how　they　treated　patients.　Physicians　and　nurses　were　willing　to　believe　that　short-range　airborne　and　long-range　fomite　are　possible　transmission　routes.　HCWs　with　a　higher　education　level　had　a　better　knowledge　of　COVID-19　but　worse　personal　protective　behaviors.　The　fact　that　HCWs　with　a　longer　work　experience　had　worse　personal　protective　behaviors　showed　that　HCWs　gradually　relax　their　personal　protective　behaviors　over　time.　Moreover,　vaccination　reduced　the　negative　effects　of　the　COVID-19　pandemic　on　how　the　HCWs　treated　patients.　Importantly,　the　survey　revealed　that　after　vaccination,　HCWs　in　China　did　not　relax　their　personal　protective　behaviors,　and　it　may　bring　a　low　potential　risk　for　following　waves　of　variant　virus　(e.g.,　delta).