Nitrogen　(N)　removal　from　high-salinity　wastewater　is　a　major　challenge.　The　aerobic-heterotrophic　nitrogen　removal　(AHNR)　process　has　been　demonstrated　to　be　feasible　for　treating　hypersaline　wastewater.　In　this　study,　Halomonas　venusta　SND-01,　a　halophilic　strain　capable　of　performing　AHNR,　was　isolated　from　saltern　sediment.　The　strain　achieved　ammonium,　nitrite,　and　nitrate　removal　efficiencies　of　98%,　81%,　and　100%,　respectively.　The　N　balance　experiment　suggests　that　this　isolate　removes　N　mainly　via　assimilation.　Various　functional　genes　related　to　N　metabolism　were　found　in　the　genome　of　the　strain,　establishing　a　complex　AHNR　pathway　that　includes　ammonium　assimilation,　heterotrophic　nitrification-aerobic　denitrification,　and　assimilatory　nitrate　reduction.　Four　key　enzymes　in　the　N　removal　process　were　successfully　expressed.　The　strain　exhibited　high-adaptability　under　C/N　ratios　of　5–15,　salinities　of　2%−10%　(m/v),　and　pH　of　6.5–9.5.　Therefore,　the　strain　shows　high　potential　for　treating　saline　wastewater　with　different　inorganic　N　compositions.　©　2023　Elsevier　Ltd