Chromatography　is　a　basic　process　in　the　current　proteomics　workflow,　and　the　retention　time　alignment　of　the　chromatogram　is　one　of　the　important　steps　to　effectively　improve　the　identification　and　quantification　accuracy.　After　years　of　development,　a　series　of　algorithms　for　retention　time　alignment　have　been　developed.　This　review　summarizes　the　advances　of　chromatographic　retention　time　alignment　algorithms　and　tools　for　proteomics　analysis　from　the　perspective　of　proteomics　users,　and　discusses　the　development　and　future　application　directions.　©　2022　Chin　J　Biotech,　All　rights　reserved