In　this　study,　four　groups　of　SBR　reactors　with　the　same　specification　was　selected　to　explore　effects　of　total　nitrogen　(TN)　and　total　phosphorus　(TP)　removal　from　aerobic　granular　sludge　under　different　anaerobic/anoxic　time　as　50/170,　90/130,　130/90,　180/40min　and　the　hydrolytic　acidification　liquid　as　the　influent　matrix　in　A/O/A　mode.　The　results　show　that　the　carbon　(C)　source　reserves　and　P　release　from　granular　sludge　increased　with　an　increment　in　the　anaerobic　time　from　50　to　90min.　The　efficiency　of　simultaneous　nitrification　and　denitrification　(SND)　increased　to　62.65%,　and　the　removal　rates　of　TN　and　TP　increased　from　81.1%　to　92.9%　and　82.2%　to　98.5%,　respectively.　When　the　anaerobic　time　increased　from　90　to　180min,　the　P　release　decreased　and　the　anaerobic　endogenous　conditions　stimulated　the　increase　in　EPS　and　the　decrease　in　PHA　synthesis,　which　led　to　a　decrease　in　the　TP　removal　rate　to　88.1%.　At　the　same　time,　when　the　anoxic　time　was　shortened　from　130　to　40min,　more　NOx-　remained　in　the　system,　resulting　in　a　reduction　in　TN　removal　rate　to　84%.　Mechanism　analysis　suggests　that　TN　was　consumed　by　denitrifying　polyphosphate　accumulating　organisms　and　denitrifying　glycogen　accumulating　organisms　in　the　aerobic　section　by　means　of　PHA　in　the　form　of　SND;　then　further　removed　by　endogenous　denitrification　of　DGAOs　in　the　anoxic　section;　and　finally,　TP　was　removed　by　PAOs　and　DPAOs.　Our　batch　experiments　demonstrated　the　highest　proportion　of　DPAOs　in　R2,　up　to　41%.　During　the　operation　of　the　four　groups　of　reactors,　the　particles　did　not　disintegrate,　implying　that　the　particles　cultured　with　hydrolytic　acidification　solution　had　complete　structure　and　strong　stability.　We　conclude　that　appropriate　extension　of　anerobic/anoxic　is　conducive　to　strengthening　the　storage　and　transformation　of　internal　C　source,　enhancing　the　effects　of　anaerobic　P　release,　SND,　and　post　endogenous　denitrification,　and　realizing　the　efficient　and　stable　operation　of　simultaneous　nitrification-endogenous　denitrification　and　P　removal.　©　2022　Chinese　Society　for　Environmental　Sciences.　All　rights　reserved.